Results 51 to 60 of about 26,517 (143)

Rad53 limits CMG helicase uncoupling from DNA synthesis at replication forks. [PDF]

Nat Struct Mol Biol, 2020
The coordination of DNA unwinding and synthesis at replication forks promotes efficient and faithful replication of chromosomal DNA. Disruption of the balance between helicase and polymerase activities during replication stress leads to fork progression defects and activation of the Rad53 checkpoint kinase, which is essential for the functional ...
Devbhandari S, Remus D.
europepmc   +4 more sources

Action of CMG with strand-specific DNA blocks supports an internal unwinding mode for the eukaryotic replicative helicase

eLife, 2017
Replicative helicases are ring-shaped hexamers that encircle DNA for duplex unwinding. The currently accepted view of hexameric helicase function is by steric exclusion, where the helicase encircles one DNA strand and excludes the other, acting as a ...
Lance Langston, Mike O’Donnell
doaj   +1 more source

Nucleotide binding halts diffusion of the eukaryotic replicative helicase during activation

Nature Communications, 2023
The eukaryotic replicative helicase CMG centrally orchestrates the replisome and leads the way at the front of replication forks. Understanding the motion of CMG on the DNA is therefore key to our understanding of DNA replication.
Daniel Ramírez Montero, Humberto Sánchez, Edo van Veen, Theo van Laar, Belén Solano, John F. X. Diffley, Nynke H. Dekker   +6 more
doaj   +1 more source

Mutation spectrum data for Saccharomyces cerevisiae psf1-1 pol2-M644G mutants

Data in Brief, 2022
DNA replication in Saccharomyces cerevisiae and other eukaryotes is performed mainly by polymerase epsilon (Pol ε) on the leading strand and polymerase delta (Pol δ) on the lagging strand.
Michal Dmowski, Karolina Makiela-Dzbenska, Malgorzata Jedrychowska, Milena Denkiewicz-Kruk, Iwona J. Fijalkowska   +4 more
doaj   +1 more source

Site-specific MCM sumoylation prevents genome rearrangements by controlling origin-bound MCM.

PLoS Genetics, 2022
Timely completion of eukaryotic genome duplication requires coordinated DNA replication initiation at multiple origins. Replication begins with the loading of the Mini-Chromosome Maintenance (MCM) complex, proceeds by the activation of the Cdc45-MCM-GINS
Yun Quan, Qian-Yi Zhang, Ann L Zhou, Yuhao Wang, Jiaxi Cai, Yong-Qi Gao, Huilin Zhou   +6 more
doaj   +1 more source

Mcm10 promotes rapid isomerization of CMG-DNA for replisome bypass of lagging strand DNA blocks

eLife, 2017
Replicative helicases in all cell types are hexameric rings that unwind DNA by steric exclusion in which the helicase encircles the tracking strand only and excludes the other strand from the ring.
Lance D Langston, Ryan Mayle, Grant D Schauer, Olga Yurieva, Daniel Zhang, Nina Y Yao, Roxana E Georgescu, Mike E O'Donnell   +7 more
doaj   +1 more source

Identification of Selective ATP-Competitive CMG Helicase Inhibitors for Cancer Intervention that Disrupt CMG-Replisome Function

, 2023
Abstract The human CMG helicase (Cdc45-MCM-GINS) is a novel target for anti-cancer therapy due to tumor-specific weaknesses in CMG function induced by oncogenic changes and the need for CMG function during recovery from replicative stresses such as chemotherapy.
Mark Alexandrow, Shengyan Xiang, Xingju Luo, Darcy Welch, Damon Reed   +4 more
openaire   +2 more sources

Myc-driven chromatin accessibility regulates Cdc45 assembly into CMG helicases [PDF]

Communications Biology, 2019
Abstract Myc-driven tumorigenesis involves a non-transcriptional role for Myc in over-activating replication origins. We show here that the mechanism underlying this process involves a direct role for Myc in activation of Cdc45-MCM-GINS (CMG) helicases at Myc-targeted sites.
Nepon-Sixt, Brook S., Bryant, Victoria L., Alexandrow, Mark G.   +2 more
openaire   +2 more sources

ATPase cycle and DNA unwinding kinetics of RecG helicase [PDF]

, 2012
The superfamily 2 bacterial helicase, RecG, is a monomeric enzyme with a role in DNA repair by reversing stalled replication forks. The helicase must act specifically and rapidly to prevent replication fork collapse. We have shown that RecG binds tightly
Webb, Martin R, Powell, Ben, Ben Powell (159692), Toseland, Christopher P., Powell Ben, Webb Martin R., Toseland Christopher P., Martin R Webb, Christopher P Toseland, Ben Powell, Christopher P. Toseland (13866), Martin R. Webb (159695)   +11 more
core   +1 more source

Structure of DNA-CMG-Pol epsilon elucidates the roles of the non-catalytic polymerase modules in the eukaryotic replisome

Nature Communications, 2018
Eukaryotic origin firing depends on assembly of the Cdc45-MCM-GINS (CMG) helicase, which requires the leading-strand polymerase Pol ɛ. Here the authors present a structural analysis of a CMG Pol ɛ on a DNA fork, providing insight on the steps leading ...
Panchali Goswami, Ferdos Abid Ali, Max E. Douglas, Julia Locke, Andrew Purkiss, Agnieszka Janska, Patrik Eickhoff, Anne Early, Andrea Nans, Alan M. C. Cheung, John F. X. Diffley, Alessandro Costa   +11 more
doaj   +1 more source