Results 51 to 60 of about 2,048 (142)
Structure of the eukaryotic replicative CMG helicase suggests a pumpjack motion for translocation. [PDF]
Nat Struct Mol Biol, 2016 The CMG helicase is composed of Cdc45, Mcm2-7 and GINS. Here we report the structure of the S. cerevisiae CMG determined by cryo-EM at a resolution of 3.7–4.8 Å. The structure reveals that GINS and Cdc45 scaffold the N-tier of the helicase while enabling motion of the AAA+ C-tier.
Yuan Z +6 more
europepmc +5 more sources
Replisome Preservation by a Single-Stranded DNA Gate in the CMG Helicase [PDF]
Biophysical Journal, 2018 ABSTRACT The eukaryotic replicative helicase CMG is assembled at replication origins and is thought to remain topologically closed until termination. Upon encountering a lesion, CMG must vacate a stalled fork to allow DNA repair. However, the fate of CMG under these stress conditions remains unclear.
Wasserman, Michael R. +3 more
openaire +2 more sources
Recombination and Pol ζ Rescue Defective DNA Replication upon Impaired CMG Helicase-Pol ε Interaction. [PDF]
Int J Mol Sci, 2020 The CMG complex (Cdc45, Mcm2–7, GINS (Psf1, 2, 3, and Sld5)) is crucial for both DNA replication initiation and fork progression. The CMG helicase interaction with the leading strand DNA polymerase epsilon (Pol ε) is essential for the preferential ...
Denkiewicz-Kruk M +6 more
europepmc +2 more sources
DDK regulates replication initiation by controlling the multiplicity of Cdc45-GINS binding to Mcm2-7
eLife, 2021 The committed step of eukaryotic DNA replication occurs when the pairs of Mcm2-7 replicative helicases that license each replication origin are activated. Helicase activation requires the recruitment of Cdc45 and GINS to Mcm2-7, forming Cdc45-Mcm2-7-GINS
Lorraine De Jesús-Kim +5 more
doaj +1 more source
eLife, 2017 Replicative helicases are ring-shaped hexamers that encircle DNA for duplex unwinding. The currently accepted view of hexameric helicase function is by steric exclusion, where the helicase encircles one DNA strand and excludes the other, acting as a ...
Lance Langston, Mike O’Donnell
doaj +1 more source
Nucleotide binding halts diffusion of the eukaryotic replicative helicase during activation
Nature Communications, 2023 The eukaryotic replicative helicase CMG centrally orchestrates the replisome and leads the way at the front of replication forks. Understanding the motion of CMG on the DNA is therefore key to our understanding of DNA replication.
Daniel Ramírez Montero +6 more
doaj +1 more source
Mechanism of DNA-protein crosslink bypass by CMG helicase
ABSTRACT DNA-protein crosslinks (DPCs) pose significant barriers to DNA replication and genome integrity. Previous studies have established that bypass of DPCs by the eukaryotic replicative helicase, CMG complex, is critical for DPC repair. We investigated the mechanism of leading-strand DPC bypass by CMG using purified components.
Kyaw MT +3 more
europepmc +2 more sources
Site-specific MCM sumoylation prevents genome rearrangements by controlling origin-bound MCM.
PLoS Genetics, 2022 Timely completion of eukaryotic genome duplication requires coordinated DNA replication initiation at multiple origins. Replication begins with the loading of the Mini-Chromosome Maintenance (MCM) complex, proceeds by the activation of the Cdc45-MCM-GINS
Yun Quan +6 more
doaj +1 more source
Mutation spectrum data for Saccharomyces cerevisiae psf1-1 pol2-M644G mutants
Data in Brief, 2022 DNA replication in Saccharomyces cerevisiae and other eukaryotes is performed mainly by polymerase epsilon (Pol ε) on the leading strand and polymerase delta (Pol δ) on the lagging strand.
Michal Dmowski +4 more
doaj +1 more source
, 2023 Abstract The human CMG helicase (Cdc45-MCM-GINS) is a novel target for anti-cancer therapy due to tumor-specific weaknesses in CMG function induced by oncogenic changes and the need for CMG function during recovery from replicative stresses such as chemotherapy.
Mark Alexandrow +4 more
openaire +2 more sources