Results 91 to 100 of about 26,517 (143)

Mcm10 regulates DNA replication elongation by stimulating the CMG replicative helicase

Genes & Development, 2017
Activation of the Mcm2–7 replicative DNA helicase is the committed step in eukaryotic DNA replication initiation. Although Mcm2–7 activation requires binding of the helicase-activating proteins Cdc45 and GINS (forming the CMG complex), an additional protein, Mcm10, drives initial origin DNA unwinding by an unknown mechanism.
Looke, Marko, Maloney, Michael Finnan, Bell, Stephen P   +2 more
openaire   +4 more sources

BRCA1 Promotes Unloading of the CMG Helicase from a Stalled DNA Replication Fork [PDF]

Molecular Cell, 2014
The tumor suppressor protein BRCA1 promotes homologous recombination (HR), a high-fidelity mechanism to repair DNA double-strand breaks (DSBs) that arise during normal replication and in response to DNA-damaging agents. Recent genetic experiments indicate that BRCA1 also performs an HR-independent function during the repair of DNA interstrand ...
Long, David T., Joukov, Vladimir, Budzowska, Magda, Walter, Johannes C.   +3 more
openaire   +2 more sources

Assembly, Activation, and Helicase Actions of MCM2-7: Transition from Inactive MCM2-7 Double Hexamers to Active Replication Forks

Biology
In this review, we summarize the processes of the assembly of multi-protein replisomes at the origins of replication. Replication licensing, the loading of inactive minichromosome maintenance double hexamers (dhMCM2-7) during the G1 phase, is followed by
Zhiying You, Hisao Masai
doaj   +1 more source

Remodeling of Interstrand Crosslink Proximal Replisomes Is Dependent on ATR, FANCM, and FANCD2

Cell Reports, 2019
Summary: Eukaryotic replisomes are driven by the mini chromosome maintenance (MCM [M]) helicase complex, an offset ring locked around the template for leading strand synthesis by CDC45 (C) and GINS (G) proteins.
Jing Huang, Jing Zhang, Marina A. Bellani, Durga Pokharel, Julia Gichimu, Ryan C. James, Himabindu Gali, Chen Ling, Zhijiang Yan, Dongyi Xu, Junjie Chen, Amom Ruhikanta Meetei, Lei Li, Weidong Wang, Michael M. Seidman   +14 more
doaj   +1 more source

Functional studies in yeast confirm the pathogenicity of a new GINS3 Meier–Gorlin syndrome variant

Clinical Genetics, Volume 106, Issue 3, Page 342-346, September 2024.
We describe the phenotypes of an individual expressing a new variant of the DNA replication protein GINS3 (Ile25Phe) and displaying features suggestive of Meier–Gorlin Syndrome. Modification of the corresponding residue in yeast Psf3 revealed partial loss of function of this variant in vivo, allowing us to upgrade its classification.
Yosra Mehrjoo, Philippe M. Campeau, Lama Al Abdi, Abdullah Aldowaish, Omar Abouyousef, Fowzan S. Alkuraya, Marta Codina‐Solà, Anna M. Cueto‐González, Hugo Wurtele   +8 more
wiley   +1 more source

Transcription as source of genetic heterogeneity in budding yeast

Yeast, Volume 41, Issue 4, Page 171-185, April 2024.
Transcription can increase genetic heterogeneity or repair damage, and interactions with DNA replication are particularly challenging. Nonetheless, DNA replication forks effectively traverse transcribed regions, and we argue that replication–transcription conflicts are only mutagenic when DNA replication fork repair events interact with transcription ...
Baptiste Piguet, Jonathan Houseley
wiley   +1 more source

CryoEM insights into RNA primer synthesis by the human primosome

The FEBS Journal, Volume 291, Issue 8, Page 1813-1829, April 2024.
Eukaryotic DNA replication depends on the primosome, a protein complex that initiates DNA synthesis by polymerisation of an RNA–DNA primer. Our research has captured the conformational changes of the human primosome under ideal biochemical conditions.
Zhan Yin, Mairi L. Kilkenny, De‐Sheng Ker, Luca Pellegrini   +3 more
wiley   +1 more source

Structure of DNA-CMG-Pol epsilon elucidates the roles of the non-catalytic polymerase modules in the eukaryotic replisome

, 2020
Eukaryotic origin firing depends on assembly of the Cdc45-MCM-GINS (CMG) helicase. A key step is the recruitment of GINS that requires the leading-strand polymerase Pol epsilon, composed of Pol2, Dpb2, Dpb3, Dpb4.
Julia Locke (8307120), Andrew Purkiss (9158504), Alan MC Cheung (9158510), Agnieszka Janska (9158507), Ferdos Abid Ali (8307117), Anne Early (8124068), Andrea Nans (5176226), Alessandro Costa (2349019), Patrik Eickhoff (8307108), John FX Diffley (8124071), Max E Douglas (9108548), Panchali Goswami (9158501)   +11 more
core  

Recognition of two distinct elements in the RNA substrate by the RNA-binding domain of the T. thermophilus DEAD box helicase Hera [PDF]

, 2013
DEAD box helicases catalyze the ATP-dependent destabilization of RNA duplexes. Whereas duplex separation is mediated by the helicase core shared by all members of the family, flanking domains often contribute to binding of the RNA substrate.
Wöhnert, Jens, Linden, Martin H., Rudolph, Markus G., Steimer, Lenz, Wurm, Jan Philip, Klostermeier, Dagmar   +5 more
core   +1 more source

Tethering of SCF(Dia2) to the Replisome Promotes Efficient Ubiquitylation and Disassembly of the CMG Helicase. [PDF]

Curr Biol, 2015
Disassembly of the Cdc45-MCM-GINS (CMG) DNA helicase, which unwinds the parental DNA duplex at eukaryotic replication forks, is the key regulated step during replication termination but is poorly understood. In budding yeast, the F-box protein Dia2 drives ubiquitylation of the CMG helicase at the end of replication, leading to a disassembly pathway ...
Maculins T, Nkosi PJ, Nishikawa H, Labib K.   +3 more
europepmc   +4 more sources