Results 91 to 100 of about 2,048 (142)

Functional studies in yeast confirm the pathogenicity of a new GINS3 Meier–Gorlin syndrome variant

Clinical Genetics, Volume 106, Issue 3, Page 342-346, September 2024.
We describe the phenotypes of an individual expressing a new variant of the DNA replication protein GINS3 (Ile25Phe) and displaying features suggestive of Meier–Gorlin Syndrome. Modification of the corresponding residue in yeast Psf3 revealed partial loss of function of this variant in vivo, allowing us to upgrade its classification.
Yosra Mehrjoo, Philippe M. Campeau, Lama Al Abdi, Abdullah Aldowaish, Omar Abouyousef, Fowzan S. Alkuraya, Marta Codina‐Solà, Anna M. Cueto‐González, Hugo Wurtele   +8 more
wiley   +1 more source

BRCA1 Promotes Unloading of the CMG Helicase from a Stalled DNA Replication Fork [PDF]

Molecular Cell, 2014
The tumor suppressor protein BRCA1 promotes homologous recombination (HR), a high-fidelity mechanism to repair DNA double-strand breaks (DSBs) that arise during normal replication and in response to DNA-damaging agents. Recent genetic experiments indicate that BRCA1 also performs an HR-independent function during the repair of DNA interstrand ...
Long, David T., Joukov, Vladimir, Budzowska, Magda, Walter, Johannes C.   +3 more
openaire   +2 more sources

Structure of DNA-CMG-Pol epsilon elucidates the roles of the non-catalytic polymerase modules in the eukaryotic replisome

, 2020
Eukaryotic origin firing depends on assembly of the Cdc45-MCM-GINS (CMG) helicase. A key step is the recruitment of GINS that requires the leading-strand polymerase Pol epsilon, composed of Pol2, Dpb2, Dpb3, Dpb4.
Julia Locke (8307120), Andrew Purkiss (9158504), Alan MC Cheung (9158510), Agnieszka Janska (9158507), Ferdos Abid Ali (8307117), Anne Early (8124068), Andrea Nans (5176226), Alessandro Costa (2349019), Patrik Eickhoff (8307108), John FX Diffley (8124071), Max E Douglas (9108548), Panchali Goswami (9158501)   +11 more
core  

Molecular mechanism of the eukaryotic replicative helicase [PDF]

, 2019
In eukaryotes, the replicative helicase CMG (Cdc45/Mcm2-7/GINS) complex separates the two strands of the double helix during DNA replication. A number of models have been proposed to explain how CMG translocates on DNA during unwinding.
Kose, Hazal Busra
core   +1 more source

Assembly, Activation, and Helicase Actions of MCM2-7: Transition from Inactive MCM2-7 Double Hexamers to Active Replication Forks

Biology
In this review, we summarize the processes of the assembly of multi-protein replisomes at the origins of replication. Replication licensing, the loading of inactive minichromosome maintenance double hexamers (dhMCM2-7) during the G1 phase, is followed by
Zhiying You, Hisao Masai
doaj   +1 more source

Remodeling of Interstrand Crosslink Proximal Replisomes Is Dependent on ATR, FANCM, and FANCD2

Cell Reports, 2019
Summary: Eukaryotic replisomes are driven by the mini chromosome maintenance (MCM [M]) helicase complex, an offset ring locked around the template for leading strand synthesis by CDC45 (C) and GINS (G) proteins.
Jing Huang, Jing Zhang, Marina A. Bellani, Durga Pokharel, Julia Gichimu, Ryan C. James, Himabindu Gali, Chen Ling, Zhijiang Yan, Dongyi Xu, Junjie Chen, Amom Ruhikanta Meetei, Lei Li, Weidong Wang, Michael M. Seidman   +14 more
doaj   +1 more source

Transcription as source of genetic heterogeneity in budding yeast

Yeast, Volume 41, Issue 4, Page 171-185, April 2024.
Transcription can increase genetic heterogeneity or repair damage, and interactions with DNA replication are particularly challenging. Nonetheless, DNA replication forks effectively traverse transcribed regions, and we argue that replication–transcription conflicts are only mutagenic when DNA replication fork repair events interact with transcription ...
Baptiste Piguet, Jonathan Houseley
wiley   +1 more source

Molecular basis for ATP-hydrolysis-driven DNA translocation by the CMG helicase of the eukaryotic replisome.

, 2020
In the eukaryotic replisome, DNA unwinding by the Cdc45-MCM-Go-Ichi-Ni-San (GINS) (CMG) helicase requires a hexameric ring-shaped ATPase named minichromosome maintenance (MCM), which spools single-stranded DNA through its central channel.
Fabrizio Martino (8307111), Julia Locke (8307120), Hasan Yardimci (8128839), Ferdos Abid Ali (8307117), Hazal B Kose (8128830), Nele Tamberg (8307123), Tatjana Petojevic (8307114), Andrea Nans (5176226), Michael R Botchan (8307129), Alessandro Costa (2349019), Patrik Eickhoff (8307108), James M Berger (8307126)   +11 more
core  

CryoEM insights into RNA primer synthesis by the human primosome

The FEBS Journal, Volume 291, Issue 8, Page 1813-1829, April 2024.
Eukaryotic DNA replication depends on the primosome, a protein complex that initiates DNA synthesis by polymerisation of an RNA–DNA primer. Our research has captured the conformational changes of the human primosome under ideal biochemical conditions.
Zhan Yin, Mairi L. Kilkenny, De‐Sheng Ker, Luca Pellegrini   +3 more
wiley   +1 more source

Differential Requirements for MCM Proteins in DNA Replication in Drosophila S2 Cells. [PDF]

, 2007
BACKGROUND: The MCM2-7 proteins are crucial components of the pre replication complex (preRC) in eukaryotes. Since they are significantly more abundant than other preRC components, we were interested in determining whether the entire cellular content was
Reina Hashimoto, Heck, Margarete M.S., Hashimoto, Reina, Sherkow, Jake, Sharron Vass, Heck, MM, Vass, Sharron, Hashimoto, R, Hashimoto Reina, Yamaguchi Masamitsu, Crevel, Gilles, Cotterill, S, Yamaguchi, M, Heck, Margarete M. S., Vass Sharron, Heck Margarete M.S., Sherkow Jake, Sherkow, Jacob S., Yamaguchi, Masamitsu, Sherkow, J, Cotterill Sue, Crevel Gilles, Margarete M S Heck, Crevel, G, Cotterill, Sue, Vass, S, Gilles Crevel, Margarete M.S. Heck, Jake Sherkow, Sue Cotterill, Masamitsu Yamaguchi   +30 more
core   +1 more source