Results 101 to 110 of about 2,048 (142)

Joint Efforts of Replicative Helicase and SSB Ensure Inherent Replicative Tolerance of G‐Quadruplex

Advanced Science, Volume 11, Issue 9, March 6, 2024.
G‐quadruplex (G4), a four‐stranded noncanonical DNA structure, has been recognized as a potent block to DNA replication. This study uncovers two G4‐overcoming replication pathways with the involvement of the core replisome only. Concisely, the replicative helicase coordinates with the SSB protein to unfold intramolecular G4 structures, paving the way ...
Lijuan Guo, Yanling Bao, Yilin Zhao, Zhiyun Ren, Lulu Bi, Xia Zhang, Cong Liu, Xi‐Miao Hou, Michelle D. Wang, Bo Sun   +9 more
wiley   +1 more source

Cancer‐associated FBXW7 loss is synthetic lethal with pharmacological targeting of CDC7

Molecular Oncology, Volume 18, Issue 2, Page 369-385, February 2024.
Using the combination of whole genome CRISPR‐Cas9 screening, RNA interference screens, proteomic mass spectrometry profiling and small molecule inhibitors, Baxter et al. demonstrate that loss of FBXW7 in tumour cells imparts a synthetic lethal effect with CDC7 inhibition.
Joseph S. Baxter, Rachel Brough, Dragomir B. Krastev, Feifei Song, Sandhya Sridhar, Aditi Gulati, John Alexander, Theodoros I. Roumeliotis, Zuza Kozik, Jyoti S. Choudhary, Syed Haider, Stephen J. Pettitt, Andrew N. J. Tutt, Christopher J. Lord   +13 more
wiley   +1 more source

Defects in the GINS complex increase the instability of repetitive sequences via a recombination-dependent mechanism.

PLoS Genetics, 2019
Faithful replication and repair of DNA lesions ensure genome maintenance. During replication in eukaryotic cells, DNA is unwound by the CMG helicase complex, which is composed of three major components: the Cdc45 protein, Mcm2-7, and the GINS complex ...
Malgorzata Jedrychowska, Milena Denkiewicz-Kruk, Malgorzata Alabrudzinska, Adrianna Skoneczna, Piotr Jonczyk, Michal Dmowski, Iwona J Fijalkowska   +6 more
doaj   +1 more source

USP37 protects mammalian cells during DNA replication stress by counteracting CUL2LRR1 and TRAIP

Cell Reports
Summary: The USP37 deubiquitylase is essential for mammalian cells to survive DNA replication stress, but the underlying mechanisms are unknown. Here, we demonstrate that USP37 binds the CDC45-MCM-GINS (CMG) helicase, which forms the stable core of the ...
Fabrizio Villa, Johanna Ainsworth, Karim P.M. Labib   +2 more
doaj   +1 more source

Initiation-specific alleles of the Cdc45 helicase-activating protein.

PLoS ONE, 2019
The committed step in DNA replication initiation is the activation of the Mcm2-7 replicative DNA helicase. Two activators, Cdc45 and GINS, associate with Mcm2-7 at origins of replication to form the CMG complex, which is the active eukaryotic replicative
Ramon Y Rios-Morales, Sze Ham Chan, Stephen P Bell   +2 more
doaj   +1 more source

Sld3CBD–Cdc45 structural insights into Cdc45 recruitment for CMG complex formation during DNA replication

eLife
DNA replication requires recruitment of Cdc45 and GINS into the MCM double hexamer by initiation factors to form an active helicase, the Cdc45–MCM–GINS (CMG) complex, at the replication origins.
Hao Li, Izumi Ishizaki, Koji Kato, Xiaomei Sun, Sachiko Muramatsu, Hiroshi Itou, Toyoyuki Ose, Hiroyuki Araki, Min Yao   +8 more
doaj   +1 more source

Hypomorphic GINS3 variants alter DNA replication and cause Meier-Gorlin syndrome

JCI Insight, 2022
The eukaryotic CDC45/MCM2-7/GINS (CMG) helicase unwinds the DNA double helix during DNA replication. The GINS subcomplex is required for helicase activity and is, therefore, essential for DNA replication and cell viability.
Mary E. McQuaid, Kashif Ahmed, Stephanie Tran, Justine Rousseau, Ranad Shaheen, Kristin D. Kernohan, Kyoko E. Yuki, Prerna Grover, Ema S. Dreseris, Sameen Ahmed, Lucie Dupuis, Jennifer Stimec, Mary Shago, Zuhair N. Al-Hassnan, Roch Tremblay, Philipp G. Maass, Michael D. Wilson, Eyal Grunebaum, Kym M. Boycott, François-Michel Boisvert, Sateesh Maddirevula, Eissa A. Faqeih, Fahad Almanjomi, Zaheer Ullah Khan, Fowzan S. Alkuraya, Philippe M. Campeau, Peter Kannu, Eric I. Campos, Hugo Wurtele   +28 more
doaj   +1 more source

USP37 prevents unscheduled replisome unloading through MCM complex deubiquitination

Nature Communications
The CMG helicase (CDC45-MCM2-7-GINS) unwinds DNA as a component of eukaryotic replisomes. Replisome (dis)assembly is tightly coordinated with cell cycle progression to ensure genome stability.
Derek L. Bolhuis, Dalia Fleifel, Thomas Bonacci, Xianxi Wang, Brandon L. Mouery, Jeanette Gowen Cook, Nicholas G. Brown, Michael J. Emanuele   +7 more
doaj   +1 more source

Poster Sessions

HemaSphere, Volume 10, Issue S1, June 2026.
wiley   +1 more source

The CMG (CDC45/RecJ, MCM, GINS) complex is a conserved component of the DNA replication system in all archaea and eukaryotes

Biology Direct, 2012
Background In eukaryotes, the CMG (CDC45, MCM, GINS) complex containing the replicative helicase MCM is a key player in DNA replication. Archaeal homologs of the eukaryotic MCM and GINS proteins have been identified but until recently no homolog of the ...
Makarova Kira S, Koonin Eugene V, Kelman Zvi   +2 more
doaj   +1 more source