Results 101 to 110 of about 26,517 (143)

Joint Efforts of Replicative Helicase and SSB Ensure Inherent Replicative Tolerance of G‐Quadruplex

Advanced Science, Volume 11, Issue 9, March 6, 2024.
G‐quadruplex (G4), a four‐stranded noncanonical DNA structure, has been recognized as a potent block to DNA replication. This study uncovers two G4‐overcoming replication pathways with the involvement of the core replisome only. Concisely, the replicative helicase coordinates with the SSB protein to unfold intramolecular G4 structures, paving the way ...
Lijuan Guo, Yanling Bao, Yilin Zhao, Zhiyun Ren, Lulu Bi, Xia Zhang, Cong Liu, Xi‐Miao Hou, Michelle D. Wang, Bo Sun   +9 more
wiley   +1 more source

Defects in the GINS complex increase the instability of repetitive sequences via a recombination-dependent mechanism.

PLoS Genetics, 2019
Faithful replication and repair of DNA lesions ensure genome maintenance. During replication in eukaryotic cells, DNA is unwound by the CMG helicase complex, which is composed of three major components: the Cdc45 protein, Mcm2-7, and the GINS complex ...
Malgorzata Jedrychowska, Milena Denkiewicz-Kruk, Malgorzata Alabrudzinska, Adrianna Skoneczna, Piotr Jonczyk, Michal Dmowski, Iwona J Fijalkowska   +6 more
doaj   +1 more source

Molecular basis for ATP-hydrolysis-driven DNA translocation by the CMG helicase of the eukaryotic replisome.

, 2020
In the eukaryotic replisome, DNA unwinding by the Cdc45-MCM-Go-Ichi-Ni-San (GINS) (CMG) helicase requires a hexameric ring-shaped ATPase named minichromosome maintenance (MCM), which spools single-stranded DNA through its central channel.
Fabrizio Martino (8307111), Julia Locke (8307120), Hasan Yardimci (8128839), Ferdos Abid Ali (8307117), Hazal B Kose (8128830), Nele Tamberg (8307123), Tatjana Petojevic (8307114), Andrea Nans (5176226), Michael R Botchan (8307129), Alessandro Costa (2349019), Patrik Eickhoff (8307108), James M Berger (8307126)   +11 more
core  

Domain orientation in the RNA helicase YxiN and the role of conformational changes for RNA unwinding [PDF]

, 2010
The RNA helicase YxiN from Bacillus subtilis is a member of the family of DEAD box proteins. YxiN is able to unwind RNA double strands in an ATP-dependent manner. The ability to catalyse RNA rearrangement is in vivo presumably necessary for the bacterial
Karow, Anne R.
core   +1 more source

The human Suv3 helicase interacts with replication protein A and flap endonuclease 1 in the nucleus [PDF]

, 2011
The human Suv3 helicase has been shown to be a major player in mitochondrial RNA surveillance and decay. In the present study we identify two new interaction partners of hSUV3: the RPA (replication protein A0 and FEN1 (flap endonuclease).
Stępień, Piotr P., Bohr, Vilhelm A, Kulikowicz, Tomasz   +2 more
core   +1 more source

USP37 protects mammalian cells during DNA replication stress by counteracting CUL2LRR1 and TRAIP

Cell Reports
Summary: The USP37 deubiquitylase is essential for mammalian cells to survive DNA replication stress, but the underlying mechanisms are unknown. Here, we demonstrate that USP37 binds the CDC45-MCM-GINS (CMG) helicase, which forms the stable core of the ...
Fabrizio Villa, Johanna Ainsworth, Karim P.M. Labib   +2 more
doaj   +1 more source

Structure of the Helicase Domain of DNA Polymerase Theta Reveals a Possible Role in the Microhomology-Mediated End-Joining Pathway [PDF]

, 2015
DNA polymerase theta (Polθ) has been identified as a crucial alternative non-homologous end-joining factor in mammalian cells. Polθ is upregulated in a range of cancer cell types defective in homologous recombination, and knockdown has been shown to ...
Aitkenhead, H, Gileadi, Opher, Cooper, Christopher D O, Newman, JA, Cooper, CD, Gileadi, O, Cooper, Christopher D.O., Newman, Joseph A., Aitkenhead, Hazel   +8 more
core   +1 more source

Sld3CBD–Cdc45 structural insights into Cdc45 recruitment for CMG complex formation during DNA replication

eLife
DNA replication requires recruitment of Cdc45 and GINS into the MCM double hexamer by initiation factors to form an active helicase, the Cdc45–MCM–GINS (CMG) complex, at the replication origins.
Hao Li, Izumi Ishizaki, Koji Kato, Xiaomei Sun, Sachiko Muramatsu, Hiroshi Itou, Toyoyuki Ose, Hiroyuki Araki, Min Yao   +8 more
doaj   +1 more source

Initiation-specific alleles of the Cdc45 helicase-activating protein.

PLoS ONE, 2019
The committed step in DNA replication initiation is the activation of the Mcm2-7 replicative DNA helicase. Two activators, Cdc45 and GINS, associate with Mcm2-7 at origins of replication to form the CMG complex, which is the active eukaryotic replicative
Ramon Y Rios-Morales, Sze Ham Chan, Stephen P Bell   +2 more
doaj   +1 more source

Monomeric PcrA helicase processively unwinds plasmid lengths of DNA in the presence of the initiator protein RepD [PDF]

, 2013
The helicase PcrA unwinds DNA during asymmetric replication of plasmids, acting with an initiator protein, in our case RepD. Detailed kinetics of PcrA activity were measured using bulk solution and a single-molecule imaging technique to investigate the ...
Fili, Natalia, Webb, Martin R, Mashanov, Gregory I, Chisty, Liisa T, Toseland, Christopher P., Dillingham, Mark S, Toseland, Christopher P, Molloy, Justin E   +7 more
core   +1 more source