Results 111 to 120 of about 2,048 (142)

Ctf4 organizes sister replisomes and Pol α into a replication factory

eLife, 2019
The current view is that eukaryotic replisomes are independent. Here we show that Ctf4 tightly dimerizes CMG helicase, with an extensive interface involving Psf2, Cdc45, and Sld5. Interestingly, Ctf4 binds only one Pol α-primase.
Zuanning Yuan, Roxana Georgescu, Ruda de Luna Almeida Santos, Daniel Zhang, Lin Bai, Nina Y Yao, Gongpu Zhao, Michael E O'Donnell, Huilin Li   +8 more
doaj   +1 more source

CryoEM structures of human CMG-ATPγS-DNA and CMG-AND-1 complexes.

, 2020
DNA unwinding in eukaryotic replication is performed by the Cdc45-MCM-GINS (CMG) helicase. Although the CMG architecture has been elucidated, its mechanism of DNA unwinding and replisome interactions remain poorly understood.

core   +1 more source

Both Chromosome Decondensation and Condensation Are Dependent on DNA Replication in C.elegans Embryos [PDF]

, 2015
During cell division, chromatin alternates between a condensed state to facilitate chromosome segregation and a decondensed form when DNA replicates.
Blow, J. Julian; id_orcid, Karim Labib, Craig, Gillian, Remi Sonneville, Labib, Karim, Sonneville, Remi, Gillian Craig, Anton Gartner, J. Julian Blow, Blow, J. Julian, Labib, Karim; id_orcid, Gartner, Anton   +11 more
core   +1 more source

USP37 prevents premature disassembly of stressed replisomes by TRAIP

Nature Communications
The eukaryotic replisome, which consists of the CDC45-MCM2-7-GINS (CMG) helicase, replicative polymerases, and several accessory factors, sometimes encounters proteinaceous obstacles that threaten genome integrity.
Olga V. Kochenova, Giuseppina D’Alessandro, Domenic Pilger, Ernst Schmid, Sean L. Richards, Marcos Rios Garcia, Satpal S. Jhujh, Andrea Voigt, Vipul Gupta, Christopher J. Carnie, R. Alex Wu, Nadia Gueorguieva, Simon Lam, Grant S. Stewart, Johannes C. Walter, Stephen P. Jackson   +15 more
doaj   +1 more source

MCM5 UFMylation regulates replication origin firing and fork progression

The EMBO Journal
Modification with UFM1 (UFMylation) is essential for cell proliferation, but its precise mechanism of action is unclear. Furthermore, the UFMylation pathway has been associated with microcephalic primordial dwarfism (MPD) disorders, and mutations ...
Zheng Li, Xingxuan Wu, Liu Liu, Shaohong Rao, Yanting Liao, Mengting Liu, Bin Peng, Qiongdan Zhang, Yisui Xia, Yuanliang Zhai, Shunichi Takeda, Xingzhi Xu   +11 more
doaj   +1 more source

EHA2024 Hybrid Congress

HemaSphere, Volume 8, Issue S1, June 2024.
wiley   +1 more source

Lnc956-TRIM28-HSP90B1 complex on replication forks promotes CMG helicase retention to ensure stem cell genomic stability and embryogenesis. [PDF]

Sci Adv, 2023
Zhang W, Tang M, Wang L, Zhou H, Gao J, Chen Z, Zhao B, Zheng P.   +7 more
europepmc   +1 more source

Family D DNA polymerase interacts with GINS to promote CMG-helicase in the archaeal replisome. [PDF]

Nucleic Acids Res, 2022
Oki K, Nagata M, Yamagami T, Numata T, Ishino S, Oyama T, Ishino Y.   +6 more
europepmc   +1 more source

Stabilization of expandable DNA repeats by the replication factor Mcm10 promotes cell viability

Nature Communications
Trinucleotide repeats, including Friedreich’s ataxia (GAA)n repeats, become pathogenic upon expansions during DNA replication and repair. Here, we show that deficiency of the essential replisome component Mcm10 dramatically elevates (GAA)n repeat ...
Chiara Masnovo, Zohar Paleiov, Daniel Dovrat, Laurel K. Baxter, Sofia Movafaghi, Amir Aharoni, Sergei M. Mirkin   +6 more
doaj   +1 more source

Targeting DNA helicase CMG complex and NFκB2-driven drug-resistant transcriptional axis to effectively treat KRASG12D-mutated pancreatic cancer

Experimental Hematology & Oncology
Pancreatic ductal adenocarcinoma (PDAC) is a devastating malignancy with a 5-year overall survival (OS) rate of approximately 12%. More than 90% of PDAC patients harbor oncogenic mutations in the Kirsten rat sarcoma viral homolog (KRAS) gene.
Jeffrey Xiao, Joshua Kim, Brandon Park, David J. Baylink, Cedric Kwon, Victoria Tran, Scott Lee, Kevin Codorniz, Laren Tan, Pamela Lobo Moreno, Amy Schill-Depew, Saied Mirshahidi, David De Semir, Diana Hanna, Kiran Naqvi, Huynh Cao, Chien-Shing Chen, Joanne Xiu, Heinz-Josef Lenz, Hamid Mirshahidi, Mark E Reeves, Yi Xu   +21 more
doaj   +1 more source