Results 11 to 20 of about 26,517 (143)
Structure of human Cdc45 and implications for CMG helicase function [PDF]
Nature Communications, 2016 The cell cycle division protein Cdc45 is required for genome duplication in eukaryotes. Here, the authors determine the crystal structure of human Cdc45 and combine it with functional data to improve our understanding of its role in DNA replication.
Aline C. Simon +3 more
doaj +7 more sources
The CMG Helicase Bypasses DNA-Protein Cross-Links to Facilitate Their Repair [PDF]
Cell, 2019 Covalent DNA-protein cross-links (DPCs) impede replication fork progression and threaten genome integrity. Using Xenopus egg extracts, we previously showed that replication fork collision with DPCs causes their proteolysis, followed by translesion DNA ...
Johannes C Walter +2 more
exaly +10 more sources
The Human Replicative Helicase, the CMG Complex, as a Target for Anti-cancer Therapy [PDF]
Frontiers in Molecular Biosciences, 2018 DNA helicases unwind or rearrange duplex DNA during replication, recombination and repair. Helicases of many pathogenic organisms such as viruses, bacteria, and protozoa have been studied as potential therapeutic targets to treat infectious diseases, and
Yeon-Soo Seo, Young-Hoon Kang
doaj +6 more sources
DONSON is required for CMG helicase assembly in the mammalian cell cycle [PDF]
EMBO Reports, 2023 Abstract DONSON is one of 13 genes mutated in a form of primordial microcephalic dwarfism known as Meier-Gorlin Syndrome. The other 12 encode components of the CDC45-MCM-GINS helicase, around which the eukaryotic replisome forms, or are factors required for helicase assembly during DNA replication initiation.
Karim Labib +2 more
exaly +6 more sources
Mechanism of replication origin melting nucleated by CMG helicase assembly. [PDF]
Nature, 2022 The activation of eukaryotic origins of replication occurs in temporally separated steps to ensure that chromosomes are copied only once per cell cycle. First, the MCM helicase is loaded onto duplex DNA as an inactive double hexamer.
Lewis JS +7 more
europepmc +4 more sources
Reconstitution of human CMG helicase ubiquitylation by CUL2LRR1 and multiple E2 enzymes. [PDF]
Biochem J, 2021 Cullin ubiquitin ligases drive replisome disassembly during DNA replication termination. In worm, frog and mouse cells, CUL2LRR1 is required to ubiquitylate the MCM7 subunit of the CMG helicase.
Le TT +4 more
europepmc +6 more sources
The mechanism of eukaryotic CMG helicase activation [PDF]
Nature, 2018 The initiation of eukaryotic DNA replication occurs in two discrete stages: first, the minichromosome maintenance (MCM) complex assembles as a head-to-head double hexamer that encircles duplex replication origin DNA during G1 phase; then, 'firing factors' convert each double hexamer into two active Cdc45-MCM-GINS helicases (CMG) during S phase.
John Diffley, Max Douglas
exaly +5 more sources
The replicative CMG helicase: the ideal target for cancer therapy [PDF]
The Ukrainian Biochemical Journal, 2020 This review focuses on Cdc45-Mcm2-7-GINS (CMG) helicase which is a key component of the cellular replication machinery and a new promising target for cancer therapy.
W. Henderson +3 more
doaj +2 more sources
The eukaryotic CMG helicase pumpjack and integration into the replisome [PDF]
Nucleus, 2016 The eukaryotic replisome is α multiprotein machine that contains DNA polymerases, sliding clamps, helicase, and primase along with several factors that participate in cell cycle and checkpoint control. The detailed structure of the 11-subunit CMG helicase (Cdc45/Mcm2-7/GINS) has been solved recently by cryoEM single-particle 3D reconstruction and ...
Huilin Li +2 more
exaly +5 more sources
Replication Fork Activation Is Enabled by a Single-Stranded DNA Gate in CMG Helicase [PDF]
Cell, 2019 The eukaryotic replicative helicase CMG is a closed ring around double-stranded (ds)DNA at origins yet must transition to single-stranded (ss)DNA for helicase action. CMG must also handle repair intermediates, such as reversed forks that lack ssDNA. Here, using correlative single-molecule fluorescence and force microscopy, we show that CMG harbors a ...
Michael O'Donnell +2 more
exaly +6 more sources