Results 31 to 40 of about 953,432 (120)

PROTAC-mediated conditional degradation of the WRN helicase as a potential strategy for selective killing of cancer cells with microsatellite instability

Scientific Reports
Multiple studies have demonstrated that cancer cells with microsatellite instability (MSI) are intolerant to loss of the Werner syndrome helicase (WRN), whereas microsatellite-stable (MSS) cancer cells are not.
Vikram Tejwani, Thomas Carroll, Thomas Macartney, Susanne Bandau, Constance Alabert, Giulia Saredi, Rachel Toth, John Rouse   +7 more
doaj   +1 more source

Localization-Dependent and -Independent Roles of SLX4 in Regulating Telomeres

Cell Reports, 2013
SLX4, a scaffold for structure-specific DNA repair nucleases, is important for several types of DNA repair. Many repair proteins bind to sites of DNA damage, resulting in subnuclear “foci,” but SLX4 forms foci in human cells even without DNA damage ...
Jamie S.J. Wilson, Agueda M. Tejera, Dennis Castor, Rachel Toth, Maria A. Blasco, John Rouse   +5 more
doaj   +1 more source

The EF-hand domain of MINDY3 is a ubiquitin and RAD23 UBL-binding domain

EMBO Reports
The MINDY family of deubiquitinases (DUBs) are exemplified by their preference for cleaving K48-linked polyubiquitin. MINDY3 is architecturally distinct from other MINDY DUBs as its catalytic domain spans the entire length of the protein except for an ...
Lee A Armstrong, Matthew R McFarland, Rachel O’Dea, Roscislaw Krutyholowa, Magdalena Gorka, Thomas Carroll, Sebastian Glatt, Yogesh Kulathu   +7 more
doaj   +1 more source

HMMR/RHAMM recruits SACK1D/FAM83D-CK1α complex at the mitotic spindle to control spindle alignment

iScience
Summary: The SACK1D/FAM83D-CK1α complex assembles at the mitotic spindle to orchestrate proper spindle positioning and error-free progression through mitosis.
Tyrell N. Cartwright, Naveen K. Nakarakanti, Karen Dunbar, Luke J. Fulcher, Selina Bader, Nicola T. Wood, Thomas J. Macartney, Gopal P. Sapkota   +7 more
doaj   +1 more source

Synthesis of Isomeric Phosphoubiquitin Chains Reveals that Phosphorylation Controls Deubiquitinase Activity and Specificity

Cell Reports, 2016
Ubiquitin is post-translationally modified by phosphorylation at several sites, but the consequences of these modifications are largely unknown. Here, we synthesize multi-milligram quantities of ubiquitin phosphorylated at serine 20, serine 57, and ...
Nicolas Huguenin-Dezot, Virginia De Cesare, Julien Peltier, Axel Knebel, Yosua Adi Kristaryianto, Daniel T. Rogerson, Yogesh Kulathu, Matthias Trost, Jason W. Chin   +8 more
doaj   +1 more source

Determination of Cellular Phosphatidylinositol-3-phosphate (PI3P) Levels Using a Fluorescently Labelled Selective PI3P Binding Domain (PX)

Bio-Protocol, 2016
The lipid Phosphatidylinositol-3-phosphate [PtdIns3P or PI(3)P] plays many membrane trafficking roles and is primarily produced by the Class III PI3K, VPS34. Determining the level of cellular PI(3)P however can be complex.
Michael Munson, Ian Ganley
doaj   +1 more source

Mechanism of TNFα-induced downregulation of salt-inducible kinase 2 in adipocytes

Scientific Reports, 2023
Salt-inducible kinase 2 (SIK2) is highly expressed in white adipocytes, but downregulated in individuals with obesity and insulin resistance. These conditions are often associated with a low-grade inflammation in adipose tissue.
Magdaléna Vaváková, Kaisa Hofwimmer, Jurga Laurencikiene, Olga Göransson   +3 more
doaj   +1 more source

Mapping the substrate landscape of protein phosphatase 2A catalytic subunit PPP2CA

iScience
Summary: Protein phosphatase 2A (PP2A) is an essential Ser/Thr phosphatase. The PP2A holoenzyme complex comprises a scaffolding (A), regulatory (B), and catalytic (C) subunit, with PPP2CA being the principal catalytic subunit.
Abigail Brewer, Gajanan Sathe, Billie E. Pflug, Rosemary G. Clarke, Thomas J. Macartney, Gopal P. Sapkota   +5 more
doaj   +1 more source

RNF12 X-Linked Intellectual Disability Mutations Disrupt E3 Ligase Activity and Neural Differentiation

Cell Reports, 2018
Summary: X-linked intellectual disability (XLID) is a heterogeneous syndrome affecting mainly males. Human genetics has identified >100 XLID genes, although the molecular and developmental mechanisms underpinning this disorder remain unclear.
Francisco Bustos, Anna Segarra-Fas, Viduth K. Chaugule, Lennart Brandenburg, Emma Branigan, Rachel Toth, Thomas Macartney, Axel Knebel, Ronald T. Hay, Helen Walden, Greg M. Findlay   +10 more
doaj   +1 more source

Phosphoproteomics identifies a bimodal EPHA2 receptor switch that promotes embryonic stem cell differentiation

Nature Communications, 2020
Fgf4 is a critical signal driving embryonic stem cell (ESC) exit from pluripotency and differentiation. Here the authors identify EPHA2 as a target of FGF4 signalling in ESCs, and show that EPHA2-EFNA1 signalling promotes pluripotent gene expression and ...
Rosalia Fernandez-Alonso, Francisco Bustos, Manon Budzyk, Pankaj Kumar, Andreas O. Helbig, Jens Hukelmann, Angus I. Lamond, Fredrik Lanner, Houjiang Zhou, Evangelia Petsalaki, Greg M. Findlay   +10 more
doaj   +1 more source