Results 41 to 50 of about 110,959 (369)

An Approach for Characterizing and Comparing Hyperspectral Microscopy Systems

open access: yesSensors, 2013
Hyperspectral imaging and analysis approaches offer accurate detection and quantification of fluorescently-labeled proteins and cells in highly autofluorescent tissues.
Silas J. Leavesley   +6 more
doaj   +1 more source

Low Power Consumption Mode Switch Based on Three-Dimensional Polymer Mach-Zehnder Interferometer

open access: yesIEEE Photonics Journal, 2020
We present a polymer thermo-optic mode switch based on the structure of two vertical Mach-Zehnder interferometers (MZIs). It can achieve a rapid switch between ${E_{00}}$, ${E_{01}}$, ${E_{10}}$ and ${E_{11}}$ with the different heaters turning on.
Jiawen Lv   +8 more
doaj   +1 more source

SUMOylation of NaV1.2 channels mediates the early response to acute hypoxia in central neurons. [PDF]

open access: yes, 2016
The mechanism for the earliest response of central neurons to hypoxia-an increase in voltage-gated sodium current (INa)-has been unknown. Here, we show that hypoxia activates the Small Ubiquitin-like Modifier (SUMO) pathway in rat cerebellar granule ...
Goldstein, Steve An   +2 more
core   +2 more sources

On the Photobleaching of Chlorophyll

open access: yesJournal of the agricultural chemical society of Japan, 1955
The photobleaching of chlorophyll which was absorbed on silica gel was examined by the irradiation of visible light (Matsuda tungsten filament lamp)under the various conditions: I. in the air with moisture. II. in N2 gas with moisture. III. in the air in dry state. IV. in N2 gas in dry state.
Keizo HAYASHIYA   +3 more
openaire   +2 more sources

Determination of diffusion coefficients in live cells using fluorescence recovery after photobleaching with wide-field fluorescence microscopy

open access: yesBiophysics and Physicobiology, 2018
Fluorescence recovery after photobleaching (FRAP) enables characterization of quantitative dynamic properties such as diffusion coefficients of fluorescent molecules in living cells by analyzing the recovery of fluorescence intensity after photobleaching
Akira Kitamura, M. Kinjo
semanticscholar   +1 more source

Influence of cell growth conditions and medium composition on EGFP photostability in live cells

open access: yesBioTechniques, 2015
Photostability is a key characteristic of fluorescent proteins. It was recently demonstrated that green fluorescent protein (GFP) photobleaching in live cells can be suppressed by changes in medium composition. Here we show that Ham's F12 medium provides
Anastasia V. Mamontova   +2 more
doaj   +1 more source

Sunlight Bleaching of Subporphyrazine Dye Films

open access: yesApplied Sciences, 2023
Stable subphthalocyanine-type dyes with a high electron affinity attract much attention as potential substitutes for traditional fullerenes in molecular electronics devices.
Vlad V. Travkin   +4 more
doaj   +1 more source

Photobleaching in STED nanoscopy and its dependence on the photon flux applied for reversible silencing of the fluorophore

open access: yesScientific Reports, 2017
In STED (stimulated emission depletion) nanoscopy, the resolution and signal are limited by the fluorophore de-excitation efficiency and photobleaching. Here, we investigated their dependence on the pulse duration and power of the applied STED light for ...
Joanna Oracz   +4 more
semanticscholar   +1 more source

Real‐time assay of ribonucleotide reductase activity with a fluorescent RNA aptamer

open access: yesFEBS Letters, EarlyView.
Ribonucleotide reductases (RNR) synthesize DNA building blocks de novo, making them crucial in DNA replication and drug targeting. FLARE introduces the first single‐tube real‐time coupled RNR assay, which enables isothermal tracking of RNR activity at nanomolar enzyme levels and allows the reconstruction of allosteric regulatory patterns and rapid ...
Jacopo De Capitani   +4 more
wiley   +1 more source

Photobleaching Effect on the Sensitivity Calibration at 638 nm of a Phosphorus-Doped Single-Mode Optical Fiber Dosimeter

open access: yesSensors
We investigated the influence of the photobleaching (PB) effect on the dosimetry performances of a phosphosilicate single-mode optical fiber (core diameter of 6.6 µm) operated at 638 nm, within the framework of the LUMINA project.
Fiammetta Fricano   +12 more
doaj   +1 more source

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