Results 81 to 90 of about 26,069 (255)

Purification, Fluorescent Labeling, and Detyrosination of Mammalian Cell Tubulin for Biochemical Assays

open access: yesCytoskeleton, EarlyView.
ABSTRACT Microtubules play essential roles in numerous cellular processes. All microtubules are built from the protein tubulin, yet individual microtubules can differ spatially and temporally due to their tubulin isotype composition and post‐translational modifications (PTMs).
Ezekiel C. Thomas   +5 more
wiley   +1 more source

Chloroplast and Cell Imaging at Submicron Resolution by Two-Photon Excitation [PDF]

open access: yes, 2012
Novel, two-photon NIR excitation fluorescence correlation microspectroscopy tests and preliminary results were presented in this article with submicron resolution for concentrated suspensions of functional cells and chloroplast membranes.
I C. Baianu
core   +1 more source

A guide to emerging technologies for large-scale and whole brain optical imaging of neuronal activity

open access: yes, 2018
The mammalian brain is a densely interconnected network that consists of millions to billions of neurons. Decoding how information is represented and processed by this neural circuitry requires the ability to capture and manipulate the dynamics of large ...
Vaziri, Alipasha   +1 more
core   +1 more source

Flashing LEDs for microalgal production [PDF]

open access: yes, 2017
Flashing lights are next-generation tools to mitigate light attenuation and increase the photosynthetic efficiency of microalgal cultivation systems illuminated by light-emitting diodes (LEDs).
Guerra, Rui Manuel Farinha das Neves   +4 more
core   +1 more source

Alpha‐Synuclein Promotes Anterograde Vesicle Transport in Melanocytes and Melanoma Cells: A Pro‐Survival Function

open access: yesJournal of the Chinese Chemical Society, EarlyView.
Model for how α‐syn modulates the positioning of endolysosomes in melanoma cells. (a) α‐syn tethers endolysosomes to the plasma membrane, a last step in anterograde transport. (b) Loss of α‐syn expression causes the loss of the tethering function, which leads to perinuclear vesicle clustering. Reproduced from the open access article.
Stephan N. Witt
wiley   +1 more source

Preoperative Skin Conditioning: Extracellular Matrix Clearance and Skin Bed Preparation, A New Paradigm. [PDF]

open access: yes, 2019
This paper introduces the concept of "skin bed preparation" prior to surgical procedures. Following the theory of chronic wound bed preparation and adapting the skin model to one of chronic wound changes related to extrinsic and intrinsic factors, a ...
Cohen, Steven R   +2 more
core  

Neuronal imaging with ultrahigh dynamic range multiphoton microscopy [PDF]

open access: yes, 2017
Multiphoton microscopes are hampered by limited dynamic range, preventing weak sample features from being detected in the presence of strong features, or preventing the capture of unpredictable bursts in sample strength.
Davison, Ian G.   +4 more
core   +1 more source

Development of Selective Plane Illumination Surface Enhanced Raman Scattering Spectral Microscopy for Improved Cellular Imaging

open access: yesJournal of Raman Spectroscopy, EarlyView.
Selective plane illumination (SPI) is combined with SERS spectral imaging to reduce background, improve image contrast, and enable improved spectroscopy from molecules on the surface of nanoparticles. This approach provides improved imaging and spectroscopy from nanoparticles in complex environments, such as biological cells.
Abigail E. Smith, Zachary D. Schultz
wiley   +1 more source

Automated Imaging of Cultured Bacteria in Picoliter Droplets by Coherent Anti‐Stokes Raman Scattering and Two‐Photon Excited Fluorescence Microscopy

open access: yesJournal of Raman Spectroscopy, EarlyView.
We present an automated system that combines an image‐based fuzzy logic controller for precise positioning of microfluidic picoliter droplets with nonlinear microscopy, using CARS and TPEF modalities for detailed analysis of droplet composition. Time series and z‐stack images of E. coli, B. subtilis, and M. arborescens were recorded.
Fabian Ott   +6 more
wiley   +1 more source

Polyphenolic Platform Ameliorated Sanshool for Skin Photoprotection

open access: yesAdvanced Science
Natural evolution has nurtured a series of active molecules that play vital roles in physiological systems, but their further applications have been severely limited by rapid deactivation, short cycle time, and potential toxicity after isolation.
Tianyou Wang   +10 more
doaj   +1 more source

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