Results 141 to 150 of about 8,200,222 (305)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

PHYSICAL-CHEMICAL BASES OF NICKEL HYDROXIDE ELECTRODE ACTIVATION

, 1992
The investigation objects are the nickel and cobalt hydroxides; hydroxocomplexes of cobalt; aqueous solutions of alkalis, nickel hydroxide electrodes. The aim is to study the physical-chemical processes proceeding in the nickel hydroxides and physical ...
Ezhov, Boris Borisovich
core  

Physical activity-associated extracellular vesicles inhibit inflammagen-induced microglial activation

Brain, Behavior, & Immunity - Health
Excessive microglial activation and neuroinflammation are pivotal contributors to the progression of neurodegenerative diseases. Physical activity has emerged as a complementary therapy recognized for its anti-inflammatory effects.
Chien-Yu Su, Heng-Juei Hsu, Tzu-Feng Wang, Hong-Lin Chou, Sheng-Feng Tsai, Chu-Wan Lee, Ching-Li Cheng, Yang-Kao Wang, Yu-Min Kuo   +8 more
doaj   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Pilot evaluation on an adapted tele-behavioral activation to increase physical activity in persons with depression: a single-arm pilot study

BMC Psychology
Background Physical activity has the potential to improve physical and mental health outcomes of persons with depression. However, feasible and acceptable strategies to integrate physical activity interventions into real-world settings are needed ...
Chad D. Rethorst, Joseph M. Trombello, Patricia M. Chen, Thomas J. Carmody, Lynnel C. Goodman, Alejandra Lazalde, Madhukar H. Trivedi   +6 more
doaj   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

From energy provision to protein synthesis: Tunnelling nanotubes as mediators of intercellular metabolic cooperation in cancer

FEBS Open Bio, EarlyView.
The cytoskeleton‐mediated transport of mitochondria via tunnelling nanotubes restores respiration, increases ATP production, rescues cells from apoptosis, activates the AKT/mTOR signalling pathway, promotes cell migration and invasiveness, contributes to cancer progression and treatment resistance.
Stanislava Martínková, Jan Trnka
wiley   +1 more source

Delivery of DNA‐encoded vaccines and proteins in mice using cutaneous suction‐mediated transfection

FEBS Open Bio, EarlyView.
Cutaneous suction‐mediated transfection in mice for delivery of DNA‐encoded vaccines and proteins. A critical limitation of DNA vaccines and other therapeutics is transfection in vivo to produce the encoded antigens or therapeutic proteins. Cutaneous suction‐based methods have demonstrated effectiveness in many animal models and have been successfully ...
Emran O. Lallow, Isabel Brandtjen, Yaxin Mo, Melissa Gulley, Louis Osorio, Sagar Kudchodkar, Nandita C. Jhumur, Christine C. Roberts, Lisa K. Denzin, David I. Shreiber, Biju Parekkadan, Hao Lin, Joel N. Maslow   +12 more
wiley   +1 more source

Enhancing Diatomaceous Earth Characteristics for Adsorption of Heavy Metals from Acid Mine Drainage: The Impact of Dual Activation Process

Jurnal Rekayasa Kimia & Lingkungan
This study investigates the effects of dual activation methods, including physical and chemical processes, on the characteristics of diatomaceous earth (DE) for the purpose of controlling heavy metal concentration in acid mine drainage.
Chalid Muksin, Farid Mulana, Nasrullah Nasrullah, Azwar Azwar, Aulia Chintia Ambarita, Muhammad Prayogie Aulia   +5 more
doaj   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source