Results 91 to 100 of about 5,655,227 (298)

Ionic liquids and deep eutectic solvents for lignocellulosic biomass fractionation [PDF]

, 2016
Lignocellulosic biomass has gained extensive research interest due to its potential as a renewable resource, which has the ability to overtake oil-based resources.
Kollau, L.J.B.M., Kollau, LJBM Laura, Alves da Rocha, MA Marisa, Asikainen, Sari, Kroon, MC, Osch, DJGP Dannie van, Bruinhorst, A Adriaan van den, van Osch, D.J.G.P., Alves da Rocha, M.A., van den Bruinhorst, A., Kroon, M.C., Asikainen, S.   +11 more
core   +2 more sources

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Harmonizing data from different soil organic matter fractionation protocols

Geoderma
Physical fractionation is a prevailing tool for studying the biogeochemical stability of soil organic carbon (SOC). Fractionation methods are straightforward to implement in the laboratory, and allow for the separation of SOC into at least two distinct ...
Tchodjowiè P.I. Kpemoua, Marija Stojanova, Claire Chenu, Denis Angers, Amicie Delahaie, Jussi Heinonsalo, Kristiina Karhu, Marine Landrieux, Sam McNally, Cédric Plessis, Christopher Poeplau, Valérie Pouteau, Pierre Roudier, Eva Rabot, Juliette Roussia, Marcus Schiedung, Iñigo Virto, Pierre Barré   +17 more
doaj   +1 more source

From energy provision to protein synthesis: Tunnelling nanotubes as mediators of intercellular metabolic cooperation in cancer

FEBS Open Bio, EarlyView.
The cytoskeleton‐mediated transport of mitochondria via tunnelling nanotubes restores respiration, increases ATP production, rescues cells from apoptosis, activates the AKT/mTOR signalling pathway, promotes cell migration and invasiveness, contributes to cancer progression and treatment resistance.
Stanislava Martínková, Jan Trnka
wiley   +1 more source

Protocol for the genomic analysis of salt-fractionated chromatin from frozen murine liver

STAR Protocols
Summary: Salt fractionation is a classical approach for separating chromatin based on its differential salt solubility and physical properties. Here, we present a protocol to apply salt fractionation for genome-scale profiling of chromatin isolated from ...
Na Yang, Sweta Sikder, Yamini Dalal, Payel Sen   +3 more
doaj   +1 more source

Delivery of DNA‐encoded vaccines and proteins in mice using cutaneous suction‐mediated transfection

FEBS Open Bio, EarlyView.
Cutaneous suction‐mediated transfection in mice for delivery of DNA‐encoded vaccines and proteins. A critical limitation of DNA vaccines and other therapeutics is transfection in vivo to produce the encoded antigens or therapeutic proteins. Cutaneous suction‐based methods have demonstrated effectiveness in many animal models and have been successfully ...
Emran O. Lallow, Isabel Brandtjen, Yaxin Mo, Melissa Gulley, Louis Osorio, Sagar Kudchodkar, Nandita C. Jhumur, Christine C. Roberts, Lisa K. Denzin, David I. Shreiber, Biju Parekkadan, Hao Lin, Joel N. Maslow   +12 more
wiley   +1 more source

Isotope fractionation during the synthesis of MDMA.HCl from helional

, 2022
An investigation into isotopic fractionation during the production of 3,4-methylenedioxymethylamphetamine (MDMA) from the novel precursor helional, and during the hydrochloric (HCl) salt precipitation of MDMA was conducted.
Cresswell, Sarah L, Carter, James F, Cormick, Justin, Currie, Timothy, Matheson, Carney   +4 more
core   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Research on Physical and Chemical Properties of Blended Bio-Oil Based on Fractionation

BioResources, 2014
To improve the properties of bio-oil, a new method was proposed that does not involve introduction of any energy medium. An ether-soluble fraction (ES), a dichloromethane soluble fraction (DCMS), and a dichloromethane insoluble fraction (DCMIS) of bio ...
Jubing Zhang, Hongmin Yang, Jianchun Jiang, Xiaoxiang Jiang   +3 more
doaj   +1 more source