Results 91 to 100 of about 59,690 (299)

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Fractionation and Characterization of Asphaltenic and Resinous Fractions of Natural Bitumen [PDF]

, 2020
In order to identify the finger prints of some fractions in butimen and to study the changes that take place when heavy oil is upgraded, precipitation, fractionation, and characterization of asphaltenes and resins from natural bitumen were carried out ...
M. T. Bisiriyu
core   +1 more source

Harmonizing data from different soil organic matter fractionation protocols

Geoderma
Physical fractionation is a prevailing tool for studying the biogeochemical stability of soil organic carbon (SOC). Fractionation methods are straightforward to implement in the laboratory, and allow for the separation of SOC into at least two distinct ...
Tchodjowiè P.I. Kpemoua, Marija Stojanova, Claire Chenu, Denis Angers, Amicie Delahaie, Jussi Heinonsalo, Kristiina Karhu, Marine Landrieux, Sam McNally, Cédric Plessis, Christopher Poeplau, Valérie Pouteau, Pierre Roudier, Eva Rabot, Juliette Roussia, Marcus Schiedung, Iñigo Virto, Pierre Barré   +17 more
doaj   +1 more source

Long‐term hippocampal alterations and cognitive impairment in a murine model of surgical sepsis

FEBS Open Bio, EarlyView.
Using a mouse model of surgical sepsis, we tested long‐term memory and analyzed the transcriptome of single cells isolated from the hippocampus. Survivor mice showed worse memory, loss of certain brain cell subpopulations, and abnormal immune cell activity—suggesting that post‐sepsis brain alterations may be linked to cognitive deficits.
Dong Seong Cho, Rebecca Schmitt, Aneesha Dasgupta, Alexandra Ducharme, Jason Doles   +4 more
wiley   +1 more source

Protocol for the genomic analysis of salt-fractionated chromatin from frozen murine liver

STAR Protocols
Summary: Salt fractionation is a classical approach for separating chromatin based on its differential salt solubility and physical properties. Here, we present a protocol to apply salt fractionation for genome-scale profiling of chromatin isolated from ...
Na Yang, Sweta Sikder, Yamini Dalal, Payel Sen   +3 more
doaj   +1 more source

Molecular dynamics simulations of positively selected codons in FcγRI reveal novel biochemical binding properties

FEBS Open Bio, EarlyView.
Evolutionary analysis across 32 placental mammals identified positive selection at residues H148 and W149 in the immune receptor FcγR1. Ancestral reconstruction combined with molecular dynamics simulations reveals how these mutations may influence receptor structure and dynamics, providing insight into the evolution of antibody recognition and immune ...
David A. Young, Omar Arias‐Gaguancela, Fiona Gibson, Morgan Grainger, Jodie Score, Amit Nathubhai, Mauricio Cafiero, Lee Richard Machado   +7 more
wiley   +1 more source

Research on Physical and Chemical Properties of Blended Bio-Oil Based on Fractionation

BioResources, 2014
To improve the properties of bio-oil, a new method was proposed that does not involve introduction of any energy medium. An ether-soluble fraction (ES), a dichloromethane soluble fraction (DCMS), and a dichloromethane insoluble fraction (DCMIS) of bio ...
Jubing Zhang, Hongmin Yang, Jianchun Jiang, Xiaoxiang Jiang   +3 more
doaj   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

STRUCTURE IDENTIFICATION OF A TRIMER STILBENOID COMPOUND FROM STEM BARK Hopea nigra (DIPTEROCARPACEAE)

Indonesian Journal of Chemistry, 2010
Bioactivity as antihephatotoxic directed fractionation of aceton extract from the stem bark of Hopea nigra (Dipterocarpaceae) afforded a stilbenoid trimer, namely vaticanol G (1).
Sri Atun, Nurfina Aznama, Retno Arianingrum, Masatake Niwab   +3 more
doaj   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source