Results 121 to 130 of about 2,060,475 (269)

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Dysfunction in Olfactory Identification and Physical Function Decline in Older Adults. [PDF]

JAMA Otolaryngol Head Neck Surg
Pan Y, Chen H, Yuan Y, Pinto JM, Couper D, Mosley TH, Shrestha S, Rowan NR, Kucharska-Newton A.   +8 more
europepmc   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Muscle fitness and physical function in children and adolescents with newly diagnosed cancer. [PDF]

Front Pediatr
Schmidt-Andersen P, Larsen HB, Pouplier A, Hansen MB, Andres-Jensen L, Lykkedegn S, Hasle H, Müller KG, Christensen J, Fridh MK.   +9 more
europepmc   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Plasma lipids connecting olfaction with cognition and physical function. [PDF]

Sci Rep
Greig EE, Resnick SM, Ferrucci L, Tian Q.   +3 more
europepmc   +1 more source

Long‐term hippocampal alterations and cognitive impairment in a murine model of surgical sepsis

FEBS Open Bio, EarlyView.
Using a mouse model of surgical sepsis, we tested long‐term memory and analyzed the transcriptome of single cells isolated from the hippocampus. Survivor mice showed worse memory, loss of certain brain cell subpopulations, and abnormal immune cell activity—suggesting that post‐sepsis brain alterations may be linked to cognitive deficits.
Dong Seong Cho, Rebecca Schmitt, Aneesha Dasgupta, Alexandra Ducharme, Jason Doles   +4 more
wiley   +1 more source

Effects of hearing intervention on physical function: A secondary analysis of the ACHIEVE study. [PDF]

PLoS One
Deal JA, Zhang W, Jiang K, Huang A, Pike JR, Arnold M, Burgard S, Chen ZS, Chisolm T, Couper D, Goman AM, Glynn NW, Gmelin T, Gravens-Mueller L, Hayden KM, Mitchell CM, Pankow JS, Reed NS, Sanchez V, Schrack JA, Sullivan K, Lin FR, Coresh J, ACHIEVE Collaborative Research Group.   +23 more
europepmc   +1 more source

Molecular dynamics simulations of positively selected codons in FcγRI reveal novel biochemical binding properties

FEBS Open Bio, EarlyView.
Evolutionary analysis across 32 placental mammals identified positive selection at residues H148 and W149 in the immune receptor FcγR1. Ancestral reconstruction combined with molecular dynamics simulations reveals how these mutations may influence receptor structure and dynamics, providing insight into the evolution of antibody recognition and immune ...
David A. Young, Omar Arias‐Gaguancela, Fiona Gibson, Morgan Grainger, Jodie Score, Amit Nathubhai, Mauricio Cafiero, Lee Richard Machado   +7 more
wiley   +1 more source

Linking FAAM and PROMIS Physical Function Scores: A Crosswalk Study. [PDF]

Foot Ankle Orthop
Nixon D, Mendenhall T, Wagers K, Kazmers N, Maughan-Egbert G.   +4 more
europepmc   +1 more source