Results 91 to 100 of about 580,444 (263)

Clinical and Functional Outcomes in Faller and Non-Faller Older Adults Clustered by Self-Organizing Maps: A Machine-Learning Approach

Applied Sciences
A wide range of outcomes makes identifying clinical and functional features distinguishing older persons who fall from non-fallers challenging, especially for professionals with less clinical experience.
Milena L. S. Almeida, Aline O. Cavalcanti, Rebeca Sarai, Mateus A. Silva, Paulo R. V. Melo, Amanda A. M. Silva, Rafael R. Caldas, Fernando Buarque, Francis Trombini-Souza   +8 more
doaj   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Resistance training as a modulator of autophagy in skeletal muscle: A systematic review of molecular mechanisms and adaptive responses

Advanced Exercise and Health Science
Autophagy is a tightly regulated intracellular degradation process essential for maintaining skeletal muscle homeostasis, particularly under metabolic stress, such as resistance exercise.
Danielle N. Ribeiro, Ana P. de Assis, Ana P. Pinto, Eduardo R. Ropelle, José R. Pauli, Ellen C. de Freitas, Adelino S.R. da Silva   +6 more
doaj   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

The impact of modern technological applications of physical fitness on improving the functional physical performance of football players. (A field study at the level of the seniors of the Spanish Barcelona Club)

مجلة العلوم و التكنولوجية للنشاطات البدنية و الرياضية
The study aims to identify the importance of modern technological applications for physical fitness in improving the functional physical performance of Barcelona senior football players.
NOUR EL ISLEM DEHAMNIA
doaj   +1 more source

Hyperactive ice‐binding proteins stabilize cell membranes and improve resistance to dehydration stress in Caenorhabditis elegans

FEBS Open Bio, EarlyView.
TisIBP8, a fungal‐derived hyperactive ice‐binding protein, helps Caenorhabditis elegans survive dehydration. It localizes near cell membranes, reduces cell damage, and helps maintain membrane structure during drying. These results suggest that ice‐binding proteins can protect cells from dehydration stress as well as freezing stress.
Daiki Shimose, Kotaro Ozaki, Ryohei Kuriyama, Yuka Ikemoto, Kazuhiro Mio, Yuji C. Sasaki, Tatsuya Arai, Sakae Tsuda, Yoichi Shinkai, Masahiro Kuramochi   +9 more
wiley   +1 more source

Review on Cathode‐Electrolyte Interphase for Stabilizing Interfaces in Solid‐State Lithium Batteries

Advanced Science
Solid‐state lithium batteries (SSLBs) hold great promise for improving battery safety and achieving higher energy density. Nevertheless, the matching diversity between solid‐state electrolytes (SEs) and the paired high‐voltage cathodes complicate to ...
Xinchao Hu, Hongfei Zheng, Chengkun Zhang, Shiyu Zhang, Yuming Jin, Dong‐Liang Peng, Qingshui Xie   +6 more
doaj   +1 more source