Results 151 to 160 of about 7,079 (185)

Accumulation of phytoene, a colorless carotenoid by inhibition of phytoene desaturase (PDS) gene in Dunaliella salina V-101

Bioresource Technology, 2017
The aim of this work was to study the accumulation of phytoene in Dunaliella salina V-101 by down-regulating its phytoene desaturase (PDS) gene expression using RNA interference and Antisense technology. RNAi and antisense constructs were introduced into the Dunaliella cells by Agrobacterium-mediated transformation.
Ramachandran Srinivasan, Subramanian Babu, Gothandam Kodiveri Muthukaliannan   +2 more
exaly   +3 more sources

In vitro carotenogenesis and characterization of the phytoene desaturase reaction inAnacystis

Biochemical and Biophysical Research Communications, 1989
An in vitro system for carotenogenesis has been developed from the cyanobacterium Anacystis. Precursor conversion is highly effective and almost no colored intermediates before beta-carotene accumulate. These cell-free reactions have been employed to characterize the phytoene desaturation reaction.
G, Sandmann, S, Kowalczyk
openaire   +2 more sources

Immunogold localization of phytoene desaturase in higher plant chloroplasts

Physiologia Plantarum, 1993
In situ location of phytoene desaturase, a key enzyme in the carotenoid biosynthesis pathway, has been investigated in chloroplasts from higher plants. For this purpose, an antiserum has been raised against the phytoene desaturase from the cyanobacterium Synechococcus PCC 7942 overexpressed in E. coli.
Hartmut Linden, M Mercedes Lucas, Gerhard Sandmann   +2 more
exaly   +2 more sources

Phytoene Desaturase, the Essential Target for Bleaching Herbicides

Weed Science, 1991
Many bleaching herbicides with different core structures inhibit phytoene desaturase (PD), a membrane-bound enzyme in the carotenogenic pathway catalyzing the hydrogen abstraction step at the first C40precursor of β-carotene. Prospects are good that new PD-active herbicides will be discovered by screening for bleaching activity.
Gerhard Sandmann, Arno Schmidt, Hartmut Linden, Peter Böger   +3 more
openaire   +1 more source

Cloning and expression analysis of phytoene desaturase and ζ-carotene desaturase genes in Carica papaya

Molecular Biology Reports, 2010
The fruit flesh color of papaya is an important nutritional quality trait and is due to the accumulation of carotenoid. To elucidate the carotenoid biosynthesis pathway in Carica papaya, the phytoene desaturase (PDS) and the ζ-carotene desaturase (ZDS) genes were isolated from papaya (named CpPDS and CpZDS) using the rapid amplification of cDNA ends ...
P, Yan, X Z, Gao, W T, Shen, P, Zhou
openaire   +2 more sources

Bleaching Herbicide Norflurazon Inhibits Phytoene Desaturase by Competition with the Cofactors

Journal of Agricultural and Food Chemistry, 2001
Cofactor requirement was determined for the heterologous expressed phytoene desaturases from the cyanobacterium Synechococcus and the higher plant Gentiana lutea. The cyanobacterial enzyme is dependent on either NAD(P) or plastoquinone, whereas only quinones such as plastoquinone can function as a cofactor for the phytoene desaturase from G.
J, Breitenbach, C, Zhu, G, Sandmann
openaire   +2 more sources

In vitro Inhibition Studies of Phytoene Desaturase by Bleaching Ketomorpholine Derivatives

Journal of Agricultural and Food Chemistry, 2000
The herbicidal activities of homochiral steroisomeric 5-methy1-2-(3-trifluoromethybenzyl)-3-keto- morpholine derivatives were investigated in vitro as inhibitors of phytoene desaturase, a key enzyme in carotenoid biosynthesis. It was demonstrated that ketomorpholines are classical bleaching compounds which directly inhibit phytoene desaturase ...
G, Sandmann, G, Mitchell
openaire   +2 more sources

Inhibition of Phytoene Desaturase – the Mode of Action of Certain Bleaching Herbicides

Zeitschrift Fur Naturforschung - Section C Journal of Biosciences, 1984
Assay systems have been developed in order to differentiate between the modes of action of certain bleaching herbicides. These include inhibition of chlorophyll or carotenoid biosynthesis, and initiation of pigment degradation. Herbicidal compounds with phytoene desaturase as their primary target site were investigated in a cell-free carotenogenic ...
Gerhard Sandmann, Peter M Bramley
exaly   +2 more sources

Phytoene Desaturase, a Target for Herbicidal Inhibitors

1989
Phytoene desaturase catalyzes the insertion of two double bonds into the first carotene in a series leading to α- and s-carotene. In several fungi this key enzyme is responsible for light/dark regulation of carotenogenesis (1). Furthermore, it is the most important target for many bleaching herbicides (2).
G. Sandmann, A. Schmidt, H. Linden, J. Hirschberger, P. Böger   +4 more
openaire   +1 more source

Phytoene desaturase is present in a large protein complex in the plastid membrane

Physiologia Plantarum, 2008
Phytoene desaturase (PDS; EC 1.14.99.‐) represents one of the key enzymes in the carotenoid biosynthetic pathway and is present in nearly all types of plastids in plants. To further characterize PDS, we isolated the PDS cDNA from cauliflower (BoPDS) and confirmed its function by heterologous expression in a strain of Escherichia coli containing a ...
Alex B, Lopez, Yong, Yang, Theodore W, Thannhauser, Li, Li   +3 more
openaire   +2 more sources