Results 21 to 30 of about 34,603 (231)

Expression of recombinant PreS2-S protein from the hepatitis B virus surface antigen in Pichia pastoris

VacciMonitor, 2021
The aim of this work is the expression of the PreS2-S region of surface antigen of hepatitis B virus (HBV) in yeast Pichia pastoris. A cDNA fragment encoding the Pres2-S protein of HBV was cloned to yeast transfer vectors.
Sobirdjan A. Sasmakov, Oybek N. Ashirov, Jaloliddin M. Abdurakhmanov, Shukhrat Sh. Khasanov, Farkhod B. Eshboev, Elvira G. Yusupova, Galina A. Piyakina, Shakhnoz S. Azimova   +7 more
doaj  

Efficient display of active lipase LipB52 with a Pichia pastoris cell surface display system and comparison with the LipB52 displayed on Saccharomyces cerevisiae cell surface

BMC Biotechnology, 2008
Background For industrial bioconversion processes, the utilization of surface-displayed lipase in the form of whole-cell biocatalysts is more advantageous, because the enzymes are displayed on the cell surface spontaneously, regarded as immobilized ...
Ma Yushu, Tao Xingyi, Ren Ren, Gao Bei, Jiang Zhengbing, Wei Dongzhi   +5 more
doaj   +1 more source

Codon pair optimization (CPO): a software tool for synthetic gene design based on codon pair bias to improve the expression of recombinant proteins in Pichia pastoris

Microbial Cell Factories, 2021
Background Codon optimization is a common method to improve protein expression levels in Pichia pastoris and the current strategy is to replace rare codons with preferred codons to match the codon usage bias.
Yide Huang, Ting Lin, Lingfang Lu, Fan Cai, Jie Lin, Yu′e Jiang, Yao Lin   +6 more
doaj   +1 more source

Maltose-binding protein enhances secretion of recombinant human granzyme B accompanied by in vivo processing of a precursor MBP fusion protein [PDF]

, 2010
Background: The apoptosis-inducing serine protease granzyme B (GrB) is an important factor contributing to lysis of target cells by cytotoxic lymphocytes.
Wels, Winfried, Pranav Oberoi, Jabulowsky, Robert Alexander, Benhar, Itai, Benjamin Dälken, Oberoi, Pranav, Robert A Jabulowsky, Itai Benhar, Winfried S Wels, Dälken, Benjamin   +9 more
core   +1 more source

Co-expressing GroEL–GroES, Ssa1–Sis1 and Bip–PDI chaperones for enhanced intracellular production and partial-wall breaking improved stability of porcine growth hormone

Microbial Cell Factories, 2020
Porcine growth hormone (pGH) is a class of peptide hormones secreted from the pituitary gland, which can significantly improve growth and feed utilization of pigs. However, it is unstable and volatile in vitro.
Jinbo Deng, Jiaoqing Li, Miaopeng Ma, Peijing Zhao, Feiping Ming, Zhipeng Lu, Juqing Shi, Qin Fan, Qianyi Liang, Junhao Jia, Jiayi Li, Shuxia Zhang, Linghua Zhang   +12 more
doaj   +1 more source

CLONING A FOREIGN GENE IN THE YEAST PICHIA PASTORIS [PDF]

, 2022
The S gene encoding the hepatitis B surface antigen was cloned into the yeast Pichia pastoris using the pPIC9 transfer vector. The recombinant plasmid pPIC9-S was treated with the restriction enzyme SacI and inserted into the yeast Pichia pastoris via ...
O.Ashirov T.Sa'dullayev A.Yarilkaganova A.Normatov S.Sasmakov Sh.Azimova
core   +1 more source

Production, Characterization and Purification of Laccase by Yeasts Isolated from Ligninolytic Soil

Journal of Pure and Applied Microbiology, 2017
Laccases are multi copper oxidases having wide substrate specificity for phenolic compounds, amines, inorganic compounds like iodine, using oxygen and producing water as by product.
S.M. Wakil, B.C. Adebayo-Tayo, O.A. Odeniyi, K.O. Salawu, S.A. Eyiolawi, A.A. Onilude   +5 more
doaj   +1 more source

Physiological manipulation and formulation of the biocontrol yeast Pichia anomala for control of Penicillium verrucosum and ochratoxin A contamination of moist grain. [PDF]

, 2008
The major hurdle in the production of commercial biocontrol agents (BCAs) has been the lack of production of appropriate formulations. Of particular importance is the conservation of viability and ecological competence after application.
Mokiou, Stella, Magan, Naresh
core   +1 more source

Raw Material Regulates Flavor Formation via Driving Microbiota in Chinese Liquor Fermentation

Frontiers in Microbiology, 2019
Raw material is important for flavors in fermented foods. Here, the effect of hulless barley on the microbiota in Chinese liquor was studied using two main cultivars (heilaoya and dulihuang).
Chongchong Liu, Chongchong Liu, Shengbao Feng, Qun Wu, Qun Wu, Heqiang Huang, Zhanxiu Chen, Shanwen Li, Yan Xu, Yan Xu   +9 more
doaj   +1 more source

Glycosylation of thrombospondin type 1 repeats [PDF]

, 2008
In the course of mutagenesis of the forth thrombospondin type 1 repeat (TSR4) of the axonal guidance protein F-spondin, one mutant designated ΔDS2,3-TSR4 missing two out of three cystein bridges was found to lack the disaccharide Glcβ1,3-Fuc-O.
Krantz, Carsten
core   +1 more source