Histochemical Staining of Collagen and Identification of Its Subtypes by Picrosirius Red Dye in Mouse Reproductive Tissues [PDF]
Collagen is one of the foremost components of tissue extracellular matrix (ECM). It provides strength, elasticity and architecture to the tissue enabling it to bear the wear and tear from external factors like physical stress as well as internal stress factors like inflammation or other pathological conditions.
Smita Bhutda +6 more
openaire +3 more sources
Effect of α7 nicotinic acetylcholine receptor activation on cardiac fibroblasts: A mechanism underlying RV fibrosis associated with cigarette smoke exposure [PDF]
INTRODUCTION: Right ventricular dysfunction is associated with numerous smoking-related illnesses including chronic obstructive pulmonary disease (COPD) where it is present even in absence of pulmonary hypertension. It is unknown if exposure to cigarette
Allawzi, Ayed +12 more
core +1 more source
Abstract Background Sodium alendronate (ALN) is widely used to treat postmenopausal osteoporosis, but both estrogen deficiency and antiresorptive therapy may impair alveolar bone repair after tooth extraction. A clinically relevant but insufficiently explored scenario involves estrogen‐deficient individuals receiving hormone replacement therapy (HRT ...
Pedro H. C. Isaias +9 more
wiley +1 more source
PURPOSE: To examine histological and histomorphometric techniques for measuring collagen in skeletal muscle. METHODS: The following staining methods were used in the study: hematoxylin and eosin, Masson's trichrome, reticulin, and picrosirius red, and ...
Eliziane Nitz de Carvalho Calvi +7 more
doaj +1 more source
Establishment of a novel ER-stress induced myopia model in mice
Background Recent studies have indicated a strong correlation between endoplasmic reticulum (ER) stress and myopia and that eyedrops containing the ER stress inducer tunicamycin (Tm) can induce myopic changes in C57BL/6 J mice.
Longdan Kang +8 more
doaj +1 more source
Quantification of connective tissue in cardiac muscle from horses used for traction using histochemical and morphometric techniques [PDF]
Os cardiomiócitos são sustentados e inseridos em um esqueleto de tecido conjuntivo, este possui distribuição desigual de acordo com as propriedades das distintas regiões em que se encontra.
Benedicto, Hildebrando Gomes +5 more
core +3 more sources
Abstract Background Free palatal gingival grafts (FGG) are commonly harvested during mucogingival procedures. The present study investigates age‐related local and systemic early secondary wound healing outcomes, following FGG harvest in a rat model. Methods A 4.2 mm diameter defect was created in the palate of 24 younger (2 m) and 24 older (6 m) Wistar
Maayan Atzmon‐Shavit +3 more
wiley +1 more source
Nicola Zerbinati,1 Alberto Calligaro2 1Department of Surgical and Morphological Sciences, University of Insubria (Varese) and Polyspecialist Medical Center, Pavia, 2Department of Public Health, Experimental and Forensic Medicine, Unit of Histology and ...
Zerbinati N, Calligaro A
doaj
Fluorescence of Picrosirius Red Multiplexed With Immunohistochemistry for the Quantitative Assessment of Collagen in Tissue Sections [PDF]
The low cost and simplicity of picrosirius red (PSR) staining have driven its popularity for collagen detection in tissue sections. We extended the versatility of this method by using fluorescent imaging to detect the PSR signal and applying automated quantification tools.
Kyle A, Wegner +3 more
openaire +2 more sources
Perfusion Cell Seeding and Expansion in Dual Mechanical Stimulation Bioreactor for In Vitro Tissue Development [PDF]
BACKGROUND: Engineered tissues are an exciting potential source of small diameter vascular grafts due to limited supply and patency of available alternatives.
Coles, Sam +2 more
core +1 more source

