Results 111 to 120 of about 212,073 (195)
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
FEBS Open Bio, EarlyView.Amino acids sequence of two different proteins with the same sequence (chameleon sequence—black boxes) represent in 3D structure of the proteins different secondary structures: HHHH—helical and BBB—Beta‐structural. The chains folded in water environment adopt different III‐order structures in which the chameleon fragments appear to adopt similar status
Irena Roterman +4 more
wiley +1 more source
The assessment of the market value of the residential house in different locations [PDF]
, 2013 The purpose of this diploma thesis is the assessment of market value of a typical detached family house in four different municipalities of Cerklje na Gorenjskem, Naklo, Radovljica and Šenčur. The market value is estimated on the basis of expense method.
Pavlin, Maja
core +1 more source
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
FEBS Open Bio, EarlyView.Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
Autism spectrum disorders in preschool children [PDF]
, 2018 Posljednjih nekoliko desetljeća bilježi se znatan porast prevalencije poremećaja iz autističnog spektra (PAS) u cijelom svijetu, što je rezultiralo i pojačanim interesom stručnjaka, znanstvenika i šire javnosti za ovaj poremećaj.
Mrđa, Nedeljka
core +1 more source
FEBS Open Bio, EarlyView.Matrix metalloproteinase‐9 (MMP9) drives ovarian cancer progression. Using MMP9‐null cells (M9‐KO) created from ovarian cancer cells, we found MMP9 loss did not block Epidermal Growth Factor (EGF)‐driven E‐cadherin dissolution or EMT but delayed and reduced EGF‐driven membrane protrusions. Transient MMP9 re‐expression drove membrane protrusion.
Claire Strauel +8 more
wiley +1 more source
Interpretive schemata of human resource management during economic crisis : case of producers for automotive industry [PDF]
, 2015 V pričujoči kvalitativni raziskavi smo se ukvarjali s kognitivnimi shemami pri udeležencih domačih podjetij, ki proizvajajo za mednarodno avtomobilsko panogo v času gospodarske krize v Sloveniji. Natančneje so nas zanimale njihove interpretativne sheme o
Arzenšek, Ana
core
FEBS Open Bio, EarlyView.This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel +4 more
wiley +1 more source
Беларускія і славацкія казкі: лінгвістычныя і тэкставая спецыфіка ўзаемаперакладу [PDF]
, 2016 Народная казка мае выразныя рысы ўніверсальнасці ў перадачы падзейнасці, ролевай суб’ектнасці і фальклорнага стылю. У адносінах славянскіх казак узровень універсальнасці павышаецца за кошт блізкароднасці моў, што знаходзіць адлюстраванне ў ...
Ляшук, Вікторыя Марленаўна
core
FEBS Open Bio, EarlyView.Despite frogs avoiding low temperatures, examination of four salamander species revealed that none avoided cold and all possessed cold tolerance. Functional analysis of TRPM8, a cold sensor, showed that all salamander TRPM8s had lost their cold sensitivity.
Tadahiro Sawao +3 more
wiley +1 more source