Transposase-assisted tagmentation of RNA/DNA hybrid duplexes
Tn5-mediated transposition of double-strand DNA has been widely utilized in various high-throughput sequencing applications. Here, we report that the Tn5 transposase is also capable of direct tagmentation of RNA/DNA hybrids in vitro.
Bo Lu +6 more
doaj +1 more source
Functional characterization and discovery of modulators of SbMATE, the agronomically important aluminium tolerance transporter from Sorghum bicolor. [PDF]
About 50% of the world's arable land is strongly acidic (pH ≤ 5). The low pH solubilizes root-toxic ionic aluminium (Al3+) species from clay minerals, driving the evolution of counteractive adaptations in cultivated crops.
Chang, Geoffrey +6 more
core +3 more sources
Fetal Brain Tumor Harboring a Unique ROCK1::BRAF Fusion
Pediatric Blood &Cancer, EarlyView.
Marllon Cindra Sant'Ana +8 more
wiley +1 more source
The newfound relationship between extrachromosomal DNAs and excised signal circles
Extrachromosomal DNAs (ecDNAs) contribute to the progression of many human cancers. In addition, circular DNA by‐products of V(D)J recombination, excised signal circles (ESCs), have roles in cancer progression but have largely been overlooked. In this Review, we explore the roles of ecDNAs and ESCs in cancer development, and highlight why these ...
Dylan Casey, Zeqian Gao, Joan Boyes
wiley +1 more source
Effect of different sowing densities in mixed cultivation of blue lupin (Lupinus angustifolius) with spring crops on yield and quality [PDF]
The aim of this investigation was to optimise the yield percentage of blue lupins in mixed cropping systems. Field experiments were conducted at two locations in Northern Germany (Institute of Organic Farming near Hamburg and Institute of Plant and Soil ...
Aulrich, Karen +3 more
core
Genetic diversity, taxonomy and legumins implications of seed storage protein profiling in Fabaceae [PDF]
Proteomic evidences can be pivotal to the discovery of new plant proteins and plant relationships, due to the diversity of form it can reveal. Seed storage protein profiles of 20 Fabaceae species: 4 grain - legumes and 16 non-pulses; of 16 genera and 10 ...
Ogunbodede, Oluwaseun O. +1 more
core +1 more source
This study reveals how the mitochondrial protein Slm35 is regulated in Saccharomyces cerevisiae. The authors identify stress‐responsive DNA elements and two upstream open reading frames (uORFs) in the 5′ untranslated region of SLM35. One uORF restricts translation, and its mutation increases Slm35 protein levels and mitophagy.
Hernán Romo‐Casanueva +5 more
wiley +1 more source
A Highly Sensitive Plant Hybrid Protein Assay System Based on the \u3cem\u3eSpm\u3c/em\u3e Promoter and TnpA Protein for Detection and Analysis of Transcription Activation Domains [PDF]
TnpA is a multifunctional DNA binding protein encoded by the maize Suppressor-mutator (Spm) transposable element. TnpA is required for transposition and is a repressor of the unmethylated Spm promoter.
Fedoroff, Nina V. +2 more
core +1 more source
Transducin beta-like gene FTL1 is essential for pathogenesis in Fusarium graminearum [PDF]
Fusarium head blight caused by Fusarium graminearum is an important disease of wheat and barley. In a previous study, we identified several mutants with reduced virulence by insertional mutagenesis. A transducin beta-like gene named FTL1 was disrupted in
Ding, S.L. +7 more
core +2 more sources
RNA binding proteins, through control of mRNA fate and expression, are key players of organism development. The LARP family of RBPs sharing the La motif, are largely present in eukaryotes. They classify into five subfamilies which members acquired specific additional domains, including the RRM1 moiety which teams up with the La motif to form a ...
openaire +3 more sources

