Results 101 to 110 of about 261,676 (351)

Diffusion‐based size determination of solute particles: a method adapted for postsynaptic proteins

open access: yesFEBS Open Bio, EarlyView.
We present a diffusion‐based approach for measuring the size of macromolecules and their complexes, and demonstrate its use on postsynaptic proteins. The method requires fluorescein‐labelled protein samples, a microfluidic device that maintains laminar flow for said samples, a microscope recording the emitted fluorescent signals, and an analytic ...
András László Szabó   +7 more
wiley   +1 more source

Next Generation Sequencing for Detection and Discovery of Plant Viruses and Viroids: Comparison of Two Approaches

open access: yesFrontiers in Microbiology, 2017
Next generation sequencing (NGS) technologies are becoming routinely employed in different fields of virus research. Different sequencing platforms and sample preparation approaches, in the laboratories worldwide, contributed to a revolution in detection
Anja Pecman   +8 more
doaj   +1 more source

Bio-Communication of Bacteria and its Evolutionary Interrelations to Natural Genome Editing Competences of Viruses [PDF]

open access: yes, 2008
Communicative competences enable bacteria to develop, organise and coordinate rich social life with a great variety of behavioral patterns even in which they organise themselves like multicellular organisms.
Guenther Witzany
core   +2 more sources

Potential and limitations of plant virus epidemiology: lessons from the Potato virus Y pathosystem [PDF]

open access: yes, 2011
Plant virus epidemiology provides powerful tools to investigate key factors that contribute to virus epidemics in agricultural crops. When successful, epidemiological approaches help to guide decisions regarding plant protection strategies.
Döring, Thomas F.
core   +1 more source

Mitochondria‐associated membranes (MAMs): molecular organization, cellular functions, and their role in health and disease

open access: yesFEBS Open Bio, EarlyView.
Mitochondria‐associated membranes (MAMs) are contact sites between the endoplasmic reticulum and mitochondria that regulate calcium signaling, lipid metabolism, autophagy, and stress responses. This review outlines their molecular organization, roles in cellular homeostasis, and how dysfunction drives neurodegeneration, metabolic disease, cancer, and ...
Viet Bui   +3 more
wiley   +1 more source

RNAi efficiency, systemic properties, and novel delivery methods for pest insect control : what we know so far [PDF]

open access: yes, 2016
In recent years, the research on the potential of using RNA interference (RNAi) to suppress crop pests has made an outstanding growth. However, given the variability of RNAi efficiency that is observed in many insects, the development of novel approaches
Christiaens, Olivier   +3 more
core   +2 more sources

Interplay between RNA‐protein interactions and RNA structures in gene regulation

open access: yesFEBS Open Bio, EarlyView.
Methodological advances in mapping transcriptome‐wide RNA‐protein interactions and RNA structures have started to uncover the potential of RNP conformations in gene regulation. Competing RNA–RNA, RNA‐protein and protein–protein interactions shape the compaction and function of RNPs throughout their lifetime and may provide novel therapeutic targets in ...
Jenni Rapakko   +2 more
wiley   +1 more source

RNA Interference in Insect Vectors for Plant Viruses

open access: yesViruses, 2016
Insects and other arthropods are the most important vectors of plant pathogens. The majority of plant pathogens are disseminated by arthropod vectors such as aphids, beetles, leafhoppers, planthoppers, thrips and whiteflies.
Surapathrudu Kanakala, Murad Ghanim
doaj   +1 more source

Cloning, expression, purification, and structural modeling of the Chandipura virus matrix protein

open access: yesFEBS Open Bio, EarlyView.
This research protocol offers a guide for the cloning, expression, and purification of the Chandipura virus matrix protein using E. coli. It also includes a step‐by‐step procedure for cloning, expressing, and conducting fluorescence imaging of GFP‐fused Chandipura virus matrix protein in mammalian cell lines.
Mariana Grieben
wiley   +1 more source

Sequence analysis and genomic organization of Aphid lethal paralysis virus: a new member of the family Dicistroviridae [PDF]

open access: yes, 2002
The complete nucleotide sequence of the genomic RNA of an aphid-infecting virus, Aphid lethal paralysis virus (ALPV), has been determined. The genome is 9812 nt in length and contains two long open reading frames (ORFs), which are separated by an ...
Clerivet, A.   +5 more
core   +2 more sources

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