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Role of Src family kinases Fyn and Lyn in arenavirus infection. [PDF]
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Enumeration of Bacteriophages by Plaque Assay
2023Enumeration of bacteriophages by plaque assay requires the mixing of host-specific bacteria with a lytic bacteriophage of interest in a soft agar overlay (top agar) to prevent the spread of viral infection in the medium; the mixture is then spread on a solid bottom agar.
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Exercise 7: Hemolytic Plaque Assay (The Jerne Plaque Assay)
2021The hemolytic plaque assay (also known as localized hemolysis in gel or Jerne plaque assay) can be used to demonstrate that individual plasma cells are monospecific. The assay permits the visualization of the small amount of antibody released in the vicinity of a single plasma cell.
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A plaque hybridization assay for rotaviruses
Journal of Virological Methods, 1989A plaque hybridization assay was adapted to rotavirus. In this test cDNA or oligonucleotide probes were used to discriminate between plaques originating from virus carrying genes of bovine and simian origin. Only mRNAs present in infected cells were detected as demonstrated by using oligonucleotides corresponding to both strands. This assay can be used
Poncet, Didier, Cohen, J.
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The Journal of Immunology, 1974
Abstract A method, the plaque cytogram assay, for preparing fixed, stained dispersions of plaque-forming cells (PFC) and rosette-forming cells (RFC) is described. Both light and scanning electron microscopy (SEM) studies demonstrate that these cells are pleomorphic.
Jerry T Thornthwaite, Robert C Leif
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Abstract A method, the plaque cytogram assay, for preparing fixed, stained dispersions of plaque-forming cells (PFC) and rosette-forming cells (RFC) is described. Both light and scanning electron microscopy (SEM) studies demonstrate that these cells are pleomorphic.
Jerry T Thornthwaite, Robert C Leif
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1987
Publisher Summary This chapter describes protein A plaque method. The plaque assay has allowed determinations of absolute frequencies of B cells producing Ig of a given type, class, subclass, and specificity among all Ig-secreting cells and, therefore, determinations of B cell repertoires.
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Publisher Summary This chapter describes protein A plaque method. The plaque assay has allowed determinations of absolute frequencies of B cells producing Ig of a given type, class, subclass, and specificity among all Ig-secreting cells and, therefore, determinations of B cell repertoires.
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A simplified plaque assay for varicella vaccine
Journal of Virological Methods, 1987A simple and accurate plaque assay is described for potency testing of attenuated varicella vaccine. Assays were performed on the African green monkey kidney continuous cell line CV-1, in multidish-plates, under a semi-solid carboxymethylcellulose overlay.
J, Husson-van Vliet +3 more
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