Results 251 to 260 of about 152,477 (295)

A simplified plaque assay for varicella vaccine

Journal of Virological Methods, 1987
A simple and accurate plaque assay is described for potency testing of attenuated varicella vaccine. Assays were performed on the African green monkey kidney continuous cell line CV-1, in multidish-plates, under a semi-solid carboxymethylcellulose overlay.
J, Husson-van Vliet, G, Colinet, F, Yane, P, Lemoine   +3 more
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Plaque assay of equine influenza virus

Veterinary Microbiology, 1984
ESK cells, a stable cell line derived from a swine embryo kidney, were found to be a good medium for plaque formation of the Prague and Miami strains of equine influenza virus. Factors influencing the plaque formation were investigated and a plaque assay for these viruses was worked out. The method is not only simple enough for routine use, but also is
H, Yamagishi, S, Ide, T, Eiki, Y, Eiguchi, T, Nagamine, Y, Igarashi, I, Yoshioka, M, Matumoto   +7 more
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Plaque Assay for Rickettsia rickettsii

Journal of Bacteriology, 1969
A plaque technique for the assay of Rickettsia rickettsii is described. The method employs primary chick or green monkey kidney monolayer cell cultures with either an agarose or special Noble agar overlay. Plaques were counted in 6 days and resultant titers correlated well with ld 50
E H, Weinberg, J R, Stakebake, P J, Gerone   +2 more
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Some variables in the plaque assay of poliovirus

Journal of Biological Standardization, 1974
Abstract Some of the variables in a plaque system for the assay of poliovirus in rhesus monkey kidney cells have been investigated and it is suggested that these should be kept in mind during the development of any similar test using a standardized cell substrate.
P B, Stones, P F, Ramsbottom
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The Plaque Assay of Animal Viruses

1962
Publisher Summary This chapter discusses many factors that contribute to the accuracy and convenience of the plaque assay. Plaque assays are introduced for animal viruses as a direct analogy with the plaque assay of bacteriophage, with the object of improving the methods of handling a wide range of animal viruses, so as to obtain more rigorous ...
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The electrophoretic hemolytic plaque assay - theory

Biophysical Chemistry, 1976
We use the mathematical theory of plaque qrowth to determine if there is merit in performing a hemolytic plaque assay in the presence of an external electric field. In particular, we study the effects of an electric field on the transport of antibodies secreted by a single lymphocyte and on the size and shape of the plaques they produce.
B, Goldstein, A S, Perelson
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Small Plaque Assay

2000
Psoriasis is a disease that is unique to man. Therefore no suitable animal models are yet available, despite the advent of transgenic animals that have raised hopes for such an eventuality. Further, as there is no good in vitro model, e.g., a cell culture system, that can replace the in vivo situation in studying medications for psoriasis, screening ...
Antti I. Lauerma, Howard I. Maibach
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An estimation of the overlap bias in plaque assay

Virology, 1966
A systematic underestimation of the density of PFU's of a virus suspension is caused by overlapping plaques. The degree of this bias depends on n, the number of plaques distributed over the petri dish and on d, the (mean) diameter of the plaques. The mean number of overlapping events, y, is proportional to n2 and to d2, y = cn2d2.
R J, Lorenz, B, Zoeth
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Direct and indirect plaque assays

1987
Publisher Summary This chapter describes direct and indirect plaque assays. All procedures described in the chapter measure the number of cells secreting specific antibody of IgM class. IgM antibody has a greater efficiency in fixing complement, thus hemolytic plaques can form directly.
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Plaque Assay for Bacteriophage

2008
The plaque assay, an indispensable tool for the study of bacteriophage, was described in the earliest publications on the discovery of these viruses. The plaque assay was validated as showing that a single phage infecting a bacterium was sufficient to initiate formation of a plaque, because there was linear proportionality between the plaques observed ...
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