Results 251 to 260 of about 230,221 (297)
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The Journal of Immunology, 1974
Abstract A method, the plaque cytogram assay, for preparing fixed, stained dispersions of plaque-forming cells (PFC) and rosette-forming cells (RFC) is described. Both light and scanning electron microscopy (SEM) studies demonstrate that these cells are pleomorphic.
Jerry T Thornthwaite, Robert C Leif
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Abstract A method, the plaque cytogram assay, for preparing fixed, stained dispersions of plaque-forming cells (PFC) and rosette-forming cells (RFC) is described. Both light and scanning electron microscopy (SEM) studies demonstrate that these cells are pleomorphic.
Jerry T Thornthwaite, Robert C Leif
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2000
Psoriasis is a disease that is unique to man. Therefore no suitable animal models are yet available, despite the advent of transgenic animals that have raised hopes for such an eventuality. Further, as there is no good in vitro model, e.g., a cell culture system, that can replace the in vivo situation in studying medications for psoriasis, screening ...
Antti I. Lauerma, Howard I. Maibach
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Psoriasis is a disease that is unique to man. Therefore no suitable animal models are yet available, despite the advent of transgenic animals that have raised hopes for such an eventuality. Further, as there is no good in vitro model, e.g., a cell culture system, that can replace the in vivo situation in studying medications for psoriasis, screening ...
Antti I. Lauerma, Howard I. Maibach
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Plaque assay of equine influenza virus
Veterinary Microbiology, 1984ESK cells, a stable cell line derived from a swine embryo kidney, were found to be a good medium for plaque formation of the Prague and Miami strains of equine influenza virus. Factors influencing the plaque formation were investigated and a plaque assay for these viruses was worked out. The method is not only simple enough for routine use, but also is
H, Yamagishi +7 more
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Herpes T virus plaque assay studies
Archiv f�r die gesamte Virusforschung, 1968A plaque assay system for the study of Herpes T virus in various host cells was examined. Squirrel monkey kidney cells were found to be the most sensitive for plaque assay. The difficulty of obtaining kidney cultures without indigenous viral agents hampered the utilization of this tissue for routine purposes and favored the use of rabbit kidney cells ...
M D, Daniel, L V, Meléndez
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1987
Publisher Summary This chapter describes protein A plaque method. The plaque assay has allowed determinations of absolute frequencies of B cells producing Ig of a given type, class, subclass, and specificity among all Ig-secreting cells and, therefore, determinations of B cell repertoires.
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Publisher Summary This chapter describes protein A plaque method. The plaque assay has allowed determinations of absolute frequencies of B cells producing Ig of a given type, class, subclass, and specificity among all Ig-secreting cells and, therefore, determinations of B cell repertoires.
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A plaque hybridization assay for rotaviruses
Journal of Virological Methods, 1989A plaque hybridization assay was adapted to rotavirus. In this test cDNA or oligonucleotide probes were used to discriminate between plaques originating from virus carrying genes of bovine and simian origin. Only mRNAs present in infected cells were detected as demonstrated by using oligonucleotides corresponding to both strands. This assay can be used
Poncet, Didier, Cohen, J.
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The electrophoretic hemolytic plaque assay - theory
Biophysical Chemistry, 1976We use the mathematical theory of plaque qrowth to determine if there is merit in performing a hemolytic plaque assay in the presence of an external electric field. In particular, we study the effects of an electric field on the transport of antibodies secreted by a single lymphocyte and on the size and shape of the plaques they produce.
B, Goldstein, A S, Perelson
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Production of monolayer plaque assay slides
Journal of Immunological Methods, 1981Abstract A jig for the simple and rapid production of monolayer plaque assay slides is described.
V L, Di Ninno +2 more
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Plaque Assay for Rickettsia rickettsii
Journal of Bacteriology, 1969A plaque technique for the assay of Rickettsia rickettsii is described. The method employs primary chick or green monkey kidney monolayer cell cultures with either an agarose or special Noble agar overlay. Plaques were counted in 6 days and resultant titers correlated well with ld 50
E H, Weinberg +2 more
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Direct and indirect plaque assays
1987Publisher Summary This chapter describes direct and indirect plaque assays. All procedures described in the chapter measure the number of cells secreting specific antibody of IgM class. IgM antibody has a greater efficiency in fixing complement, thus hemolytic plaques can form directly.
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