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Enumeration of Bacteriophages by Plaque Assay
2023Enumeration of bacteriophages by plaque assay requires the mixing of host-specific bacteria with a lytic bacteriophage of interest in a soft agar overlay (top agar) to prevent the spread of viral infection in the medium; the mixture is then spread on a solid bottom agar.
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Plaque Assay for Bacteriophage
2008The plaque assay, an indispensable tool for the study of bacteriophage, was described in the earliest publications on the discovery of these viruses. The plaque assay was validated as showing that a single phage infecting a bacterium was sufficient to initiate formation of a plaque, because there was linear proportionality between the plaques observed ...
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Improved Plaque Assay for Rickettsia tsutsugamushi
The American Journal of Tropical Medicine and Hygiene, 1987The assay of Rickettsia tsutsugamushi infectivity by plaquing has been improved substantially by a number of changes which were based on our understanding of factors which enhance scrub typhus rickettsial infection of, and replication in, cultured cells.
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The Plaque Assay of Animal Viruses
1962Publisher Summary This chapter discusses many factors that contribute to the accuracy and convenience of the plaque assay. Plaque assays are introduced for animal viruses as a direct analogy with the plaque assay of bacteriophage, with the object of improving the methods of handling a wide range of animal viruses, so as to obtain more rigorous ...
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Reverse Hemolytic Plaque Assay
Pathology - Research and Practice, 1991S. Yamada +5 more
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