Results 1 to 10 of about 81,993 (89)
VaccinesThe COVID-19 pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has resulted in more than seven million deaths worldwide. To reduce viral spread, the Israel Institute for Biological Research (IIBR) developed and produced a new rVSV-SARS-CoV-2-S vaccine candidate (BriLife®) based on a platform of a genetically engineered ...
Lilach Cherry, Edith Lupu, Eyal Dor
exaly +4 more sources
, 2020 This is part 3.6 of the "Study of MAIT Cell Activation in Viral Infections In Vivo" collection of protocols. Collection Abstract: MAIT cells are abundant, highly evolutionarily conserved innate-like lymphocytes expressing a semi-invariant T cell receptor (TCR), which recognizes microbially derived small intermediate molecules from the riboflavin ...
Timothy S C Hinks +7 more
openaire +1 more source
Variables affecting viral plaque formation in microculture plaque assays using homologous antibody in a liquid overlay [PDF]
Journal of Clinical Microbiology, 1977 A liquid antibody microculture plaque assay and the variables that govern its effectiveness are described. The assay is based on the principle that low concentrations of homologous antibody can inhibit secondary plaque formation without inhibiting formation of primary plaques. Thus, clear plaques that followed a linear dose response were produced.
A S, Randhawa +3 more
openaire +2 more sources
Viral Concentration Determination Through Plaque Assays: Using Traditional and Novel Overlay Systems [PDF]
Journal of Visualized Experiments, 2014 Plaque assays remain one of the most accurate methods for the direct quantification of infectious virons and antiviral substances through the counting of discrete plaques (infectious units and cellular dead zones) in cell culture. Here we demonstrate how to perform a basic plaque assay, and how differing overlays and techniques can affect plaque ...
Alan, Baer, Kylene, Kehn-Hall
openaire +2 more sources
A Useful Quantitative Semimicromethod for Viral Plaque Assay
Experimental Biology and Medicine, 1973 This paper presents a general method for rapid, easy, and convenient quantitation of virus titers which we have utilized in applications varying from measurement of virus distribution in infection in vivo to assay of neutralizing antibody or antiviral agents.
B, Rager-Zisman, T C, Merigan
openaire +2 more sources
New low-viscosity overlay medium for viral plaque assays.
Virology journal, 2006 Plaque assays in cell culture monolayers under solid or semisolid overlay media are commonly used for quantification of viruses and antiviral substances. To overcome the pitfalls of known overlays, we tested suspensions of microcrystalline cellulose Avicel RC/CLtrade mark as overlay media in the plaque and plaque-inhibition assay of influenza viruses ...
Garten Wolfgang +3 more
openaire +3 more sources
Standardization of Quantitative Plaque-Based Viral Assays for Orthoflavivirus Cacipacoré
VirusesCacipacoré virus (CPCV) is an understudied orthoflavivirus with significant gaps in research that hinders our understanding of its ecology, host range, and potential public health impact. A notable barrier to filling this gap is the absence of standardized methodologies for viral quantification, such as plaque-forming and focus-forming assays.
Marielena Vogel Saivish +6 more
openaire +2 more sources
Plaque Assay For Screening Viral Concentrates for Bacteriophage v1 [PDF]
, 2017 Please contact Dr. Steven Wilhelm (wilhelm@utk.edu) for additional information regarding this protocol.
openaire +1 more source
Viral Titration of SARS-COV-2 by Plaque Assay (Semi-Solid Agarose) v1
, 2020 This protocol outlines the process of plaque assay for the viral titration of SARS-CoV-2.
openaire +1 more source