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VaccinesThe COVID-19 pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has resulted in more than seven million deaths worldwide. To reduce viral spread, the Israel Institute for Biological Research (IIBR) developed and produced a ...
Einat Toister +14 more
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An Optimized High-Throughput Immuno-Plaque Assay for SARS-CoV-2
Frontiers in Microbiology, 2021 Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has been identified as the causative agent of coronavirus disease 2019 and is capable of human-to-human transmission and rapid global spread. The rapid emergence and global spread of SARS-CoV-2
Alberto A. Amarilla +20 more
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New low-viscosity overlay medium for viral plaque assays
Virology Journal, 2006 Background Plaque assays in cell culture monolayers under solid or semisolid overlay media are commonly used for quantification of viruses and antiviral substances.
Garten Wolfgang +3 more
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Standardization of the Filovirus Plaque Assay for Use in Preclinical Studies
Viruses, 2012 The filovirus plaque assay serves as the assay of choice to measure infectious virus in a cell culture, blood, or homogenized tissue sample. It has been in use for more than 30 years and is the generally accepted assay used to titrate virus in samples ...
Gene G. Olinger +10 more
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Machine-learning-based automated quantification machine for virus plaque assay counting [PDF]
PeerJ Computer Science, 2022 The plaque assay is a standard quantification system in virology for verifying infectious particles. One of the complex steps of plaque assay is the counting of the number of viral plaques in multiwell plates to study and evaluate viruses.
Gridsada Phanomchoeng +6 more
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Identification and characterisation of SARS-CoV-2 and Human alphaherpesvirus 1 from a productive coinfection in a fatal COVID-19 case [PDF]
Memorias do Instituto Oswaldo Cruz, 2022 BACKGROUND During routine Coronavirus disease 2019 (COVID-19) diagnosis, an unusually high viral load was detected by reverse transcription real-time polymerase chain reaction (RT-qPCR) in a nasopharyngeal swab sample collected from a patient with ...
Alice Laschuk Herlinger +20 more
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, 2020 This is part 3.6 of the "Study of MAIT Cell Activation in Viral Infections In Vivo" collection of protocols. Collection Abstract: MAIT cells are abundant, highly evolutionarily conserved innate-like lymphocytes expressing a semi-invariant T cell receptor (TCR), which recognizes microbially derived small intermediate molecules from the riboflavin ...
Timothy S C Hinks +7 more
openaire +1 more source
Accelerated and improved quantification of lymphocytic choriomeningitis virus (LCMV) titers by flow cytometry. [PDF]
PLoS ONE, 2012 Lymphocytic choriomeningitis virus (LCMV), a natural murine pathogen, is a member of the Arenavirus family, may cause atypical meningitis in humans, and has been utilized extensively as a model pathogen for the study of virus-induced disease and immune ...
Darlynn Korns Johnson, Dirk Homann
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Biomedicines, 2021 Engineered vaccinia virus (VACV) strains are used extensively as vectors for the development of novel cancer vaccines and cancer therapeutics. In this study, we describe for the first time a high-throughput approach for both fluorescent rVACV generation ...
Mingyu Ye +3 more
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SARS-CoV-2 coinfection in immunocompromised host leads to the generation of recombinant strain
International Journal of Infectious Diseases, 2023 Objectives: Recombination related to coinfection is a huge driving force in determining the virus genetic variability, particularly in conditions of partial immune control, leading to prolonged infection.
Silvia Zannoli +13 more
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