Evaluating Probenecid or Oseltamivir Inhibition of Influenza A Virus Replication Through Plaque Assay or Fluorescent Focus Assay Using Non-Structural Protein 1–H1N1 Venus Reporter Virus [PDF]
VirusesIt is essential to understand the molecular mechanisms of influenza antiviral therapeutics to evaluate their efficacy. Virus plaque assays are commonly used to assess the antiviral effects of drugs on virus replication; however, this method is labor ...
Jackelyn Murray +5 more
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Machine-learning-based automated quantification machine for virus plaque assay counting [PDF]
PeerJ Computer Science, 2022 The plaque assay is a standard quantification system in virology for verifying infectious particles. One of the complex steps of plaque assay is the counting of the number of viral plaques in multiwell plates to study and evaluate viruses.
Gridsada Phanomchoeng +6 more
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Validation of the Filovirus Plaque Assay for Use in Preclinical Studies [PDF]
Viruses, 2016 A plaque assay for quantitating filoviruses in virus stocks, prepared viral challenge inocula and samples from research animals has recently been fully characterized and standardized for use across multiple institutions performing Biosafety Level 4 (BSL ...
Amy C. Shurtleff +7 more
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Plaque assay for murine norovirus. [PDF]
J Vis Exp, 2012 Murine norovirus (MNV) is the only member of the Norovirus genus that efficiently grows in tissue culture. Cell lysis and cytopathic effect (CPE) are observed during MNV-1 infection of murine dendritic cells or macrophages. This property of MNV-1 can be used to quantify the number of infectious particles in a given sample by performing a plaque assay ...
Gonzalez-Hernandez MB +2 more
europepmc +4 more sources
A Fluorescent Real-Time Plaque Assay Enables Single-Cell Analysis of Virus-Induced Cytopathic Effect by Live-Cell Imaging [PDF]
Viruses, 2021 Conventional plaque assays rely on the use of overlays to restrict viral infection allowing the formation of distinct foci that grow in time as the replication cycle continues leading to countable plaques that are visualized with standard techniques such
Jorge L. Arias-Arias +2 more
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Standardization of the Filovirus Plaque Assay for Use in Preclinical Studies
Viruses, 2012 The filovirus plaque assay serves as the assay of choice to measure infectious virus in a cell culture, blood, or homogenized tissue sample. It has been in use for more than 30 years and is the generally accepted assay used to titrate virus in samples ...
Gene G. Olinger +10 more
doaj +3 more sources
Comparison of in vitro antibiotic susceptibility testing of R. typhi using plaque assay and quantitative real-time PCR [PDF]
Microbiology SpectrumMurine typhus, caused by Rickettsia typhi, is a globally distributed flea-borne disease. Antimicrobial susceptibility data are largely based on reference strains, with little evaluation of variability across clinical isolates.
Weerawat Phuklia +10 more
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Detection of Zika Virus in Desiccated Mosquitoes by Real-Time Reverse Transcription PCR and Plaque Assay [PDF]
Emerging Infectious Diseases, 2017 We assayed Zika virus–infected mosquitoes stored at room temperature for
Kristen L. Burkhalter, Harry M. Savage
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Quantification of Infectious Sendai Virus Using Plaque Assay [PDF]
Bio-Protocol, 2018 Sendai virus (SeV) is an enveloped, single-stranded RNA virus of the family Paramyxoviridae. SeV is a useful tool to study its infectious pathomechanism in immunology and the pathomechanism of a murine model of IgA nephropathy.
Narihito Tatsumoto +3 more
doaj +2 more sources
Improved Zika virus plaque assay using Vero/TMPRSS2 cell line [PDF]
Microbiology SpectrumPlaque assay is the gold standard for the quantification of viable cytopathic viruses like Zika virus (ZIKV). Some strains of ZIKV produce plaques that are very difficult to accurately visualize and count on the commonly used Vero cell line.
Olukunle O. Oluwasemowo +3 more
doaj +2 more sources