Standardization of the Filovirus Plaque Assay for Use in Preclinical Studies [PDF]
Viruses, 2012 The filovirus plaque assay serves as the assay of choice to measure infectious virus in a cell culture, blood, or homogenized tissue sample. It has been in use for more than 30 years and is the generally accepted assay used to titrate virus in samples ...
Gene G. Olinger +10 more
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Evaluating Probenecid or Oseltamivir Inhibition of Influenza A Virus Replication Through Plaque Assay or Fluorescent Focus Assay Using Non-Structural Protein 1–H1N1 Venus Reporter Virus [PDF]
VirusesIt is essential to understand the molecular mechanisms of influenza antiviral therapeutics to evaluate their efficacy. Virus plaque assays are commonly used to assess the antiviral effects of drugs on virus replication; however, this method is labor ...
Jackelyn Murray +5 more
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Machine-learning-based automated quantification machine for virus plaque assay counting [PDF]
PeerJ Computer Science, 2022 The plaque assay is a standard quantification system in virology for verifying infectious particles. One of the complex steps of plaque assay is the counting of the number of viral plaques in multiwell plates to study and evaluate viruses.
Gridsada Phanomchoeng +6 more
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Validation of the Filovirus Plaque Assay for Use in Preclinical Studies [PDF]
Viruses, 2016 A plaque assay for quantitating filoviruses in virus stocks, prepared viral challenge inocula and samples from research animals has recently been fully characterized and standardized for use across multiple institutions performing Biosafety Level 4 (BSL ...
Amy C. Shurtleff +7 more
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A Fluorescent Real-Time Plaque Assay Enables Single-Cell Analysis of Virus-Induced Cytopathic Effect by Live-Cell Imaging [PDF]
Viruses, 2021 Conventional plaque assays rely on the use of overlays to restrict viral infection allowing the formation of distinct foci that grow in time as the replication cycle continues leading to countable plaques that are visualized with standard techniques such
Jorge L. Arias-Arias +2 more
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Plaque assay for murine norovirus. [PDF]
J Vis Exp, 2012 Murine norovirus (MNV) is the only member of the Norovirus genus that efficiently grows in tissue culture. Cell lysis and cytopathic effect (CPE) are observed during MNV-1 infection of murine dendritic cells or macrophages. This property of MNV-1 can be used to quantify the number of infectious particles in a given sample by performing a plaque assay ...
Gonzalez-Hernandez MB +2 more
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Detection of Zika Virus in Desiccated Mosquitoes by Real-Time Reverse Transcription PCR and Plaque Assay [PDF]
Emerging Infectious Diseases, 2017 We assayed Zika virus–infected mosquitoes stored at room temperature for
Kristen L. Burkhalter, Harry M. Savage
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Quantification of Infectious Sendai Virus Using Plaque Assay [PDF]
Bio-Protocol, 2018 Sendai virus (SeV) is an enveloped, single-stranded RNA virus of the family Paramyxoviridae. SeV is a useful tool to study its infectious pathomechanism in immunology and the pathomechanism of a murine model of IgA nephropathy.
Narihito Tatsumoto +3 more
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Improved Zika virus plaque assay using Vero/TMPRSS2 cell line [PDF]
Microbiology SpectrumPlaque assay is the gold standard for the quantification of viable cytopathic viruses like Zika virus (ZIKV). Some strains of ZIKV produce plaques that are very difficult to accurately visualize and count on the commonly used Vero cell line.
Olukunle O. Oluwasemowo +3 more
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Antiherpevirus activity of
Annals of Clinical Microbiology and Antimicrobials, 2007 Background New prophylactic and therapeutic tools are needed for the treatment of herpes simplex virus infections. Several essential oils have shown to possess antiviral activity in vitro against a wide spectrum of viruses.
Casu Laura +6 more
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