Results 11 to 20 of about 144,914 (256)

A comprehensive method for quantifying human cytomegalovirus plaque assays [PDF]

open access: yesFrontiers in Cellular and Infection Microbiology
Human cytomegalovirus (hCMV) is an important human pathogen accounting for significant morbidity in immunocompromised individuals from neonates to cancer patients.
Laurel E. Kelnhofer-Millevolte   +8 more
doaj   +2 more sources

Antiviral Activity of Marine Actinomycetes, Saccharopolyspora jiangxiensis IMA1 against Influenza A/(H1N1) pdm09

open access: yesJournal of Pure and Applied Microbiology, 2023
Influenza viruses are major communicable pathogens responsible for respiratory diseases affecting millions worldwide and denoted by increased morbidity and significant mortality.
Kiruba Ramesh   +6 more
doaj   +1 more source

Label-Free Detection of Human Coronaviruses in Infected Cells Using Enhanced Darkfield Hyperspectral Microscopy (EDHM)

open access: yesJournal of Imaging, 2022
Human coronaviruses (HCoV) are causative agents of mild to severe intestinal and respiratory infections in humans. In the last 15 years, we have witnessed the emergence of three zoonotic, highly pathogenic HCoVs.
Devadatta Gosavi   +2 more
doaj   +1 more source

Water-in-oil droplet-mediated method for detecting and isolating infectious bacteriophage particles via fluorescent staining

open access: yesFrontiers in Microbiology, 2023
Bacteriophages are the most abundant entities on Earth. In contrast with the number of phages considered to be in existence, current phage isolation and screening methods lack throughput. Droplet microfluidic technology has been established as a platform
Miu Hoshino   +9 more
doaj   +1 more source

Accelerated and improved quantification of lymphocytic choriomeningitis virus (LCMV) titers by flow cytometry. [PDF]

open access: yesPLoS ONE, 2012
Lymphocytic choriomeningitis virus (LCMV), a natural murine pathogen, is a member of the Arenavirus family, may cause atypical meningitis in humans, and has been utilized extensively as a model pathogen for the study of virus-induced disease and immune ...
Darlynn Korns Johnson, Dirk Homann
doaj   +1 more source

Efficient Selection of Recombinant Fluorescent Vaccinia Virus Strains and Rapid Virus Titer Determination by Using a Multi-Well Plate Imaging System

open access: yesBiomedicines, 2021
Engineered vaccinia virus (VACV) strains are used extensively as vectors for the development of novel cancer vaccines and cancer therapeutics. In this study, we describe for the first time a high-throughput approach for both fluorescent rVACV generation ...
Mingyu Ye   +3 more
doaj   +1 more source

Automation of Infectious Focus Assay for Determination of Filovirus Titers and Direct Comparison to Plaque and TCID50 Assays

open access: yesMicroorganisms, 2021
Ongoing efforts to develop effective therapies against filoviruses rely, to different extents, on quantifying the amount of viable virus in samples by plaque, TCID50, and focus assays.
Patrick T. Keiser   +4 more
doaj   +1 more source

Plaque Assay for Ebola Virus [PDF]

open access: yesJournal of Clinical Microbiology, 1981
A plaque assay for Ebola virus is reported. The procedure has real potential for future research, although it is less sensitive than indirect fluorescent-antibody and mouse inoculation tests.
J B, Moe, R D, Lambert, H W, Lupton
openaire   +2 more sources

A flow cytometry-based assay for quantifying non-plaque forming strains of yellow fever virus. [PDF]

open access: yesPLoS ONE, 2012
Primary clinical isolates of yellow fever virus can be difficult to quantitate by standard in vitro methods because they may not form discernable plaques or induce a measurable cytopathic effect (CPE) on cell monolayers. In our hands, the Dakar strain of
Erika Hammarlund   +6 more
doaj   +1 more source

The antiviral activity of Laportea decumana Methanolic extract against NDV virus [PDF]

open access: yesPharmaciana
The avian species virus that causes Newcastle disease is an extremely contagious illness. Avian paramyxovirus 1, or Newcastle disease virus (NDV), is a virus that brings harm to poultry's central nervous system and digestive tract. The NDV outbreak was
Tee Albert   +4 more
doaj   +1 more source

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