Results 41 to 50 of about 230,221 (297)
Antiherpes simplex virus type 2 activity of the antimicrobial peptide subtilosin [PDF]
, 2014 In the present study we evaluated the antiviral activity of subtilosin, a cyclical peptide isolated from Bacillus amyloliquefaciens, against herpes simplex virus type 2 (HSV-2) in cell cultures and we investigated subtilosin mode of action. We determined,
Castilla, Viviana +5 more
core +1 more source
Investigating bacteriophages targeting the opportunistic pathogen Acinetobacter baumannii [PDF]
, 2020 The multi-drug resistance of the opportunistic pathogen Acinetobacter baumannii is of growing concern, with many clinical isolates proving to be resistant to last resort as well as front line antibiotic treatments.
Christie, Gabrielle S. +10 more
core +1 more source
, 2020 Bacteriophages (phage) are viruses that infect bacteria and can be found anywhere that bacteria is found. Performing plaque assays is a technique to purify a population of viruses and can also be used to determine the viral concentration of a given sample. This protocol will outline the steps to carry out plaque assays.
openaire +1 more source
New low-viscosity overlay medium for viral plaque assays
Virology Journal, 2006 Background Plaque assays in cell culture monolayers under solid or semisolid overlay media are commonly used for quantification of viruses and antiviral substances.
Garten Wolfgang +3 more
doaj +1 more source
Enzyme-linked immunosorbent assay-format tissue culture infectious dose-50 test for titrating dengue virus. [PDF]
PLoS ONE, 2011 A dengue nonstructural protein 1 (NS1) antigen capture enzyme-linked immunosorbent assay (ELISA)-based tissue culture infectious dose-50 (TCID(50)) test (TCID(50)-ELISA) was developed as an alternative to the standard plaque assay for titrating dengue ...
Jie Li +6 more
doaj +1 more source
Infection Counter: Automated Quantification of in Vitro Virus Replication by Fluorescence Microscopy
Viruses, 2016 The ability to accurately and reliably quantify viral infection is essential to basic and translational virology research. Here, we describe a simple and robust automated method for using fluorescence microscopy to estimate the proportion of virally ...
Siân Culley +4 more
doaj +1 more source
Enhancement of vaccinia virus based oncolysis with histone deacetylase inhibitors [PDF]
, 2010 Histone deacetylase inhibitors (HDI) dampen cellular innate immune response by decreasing interferon production and have been shown to increase the growth of vesicular stomatitis virus and HSV.
Atkins, H +15 more
core +1 more source
Plaque Assay of Bovine Rotavirus
Acta Veterinaria Scandinavica, 1983 Incorporation of trypsin and diethylaminoethyl-dextran in the overlay was found to be necessary for infectivity assay of the UK strain of bovine rotavirus by plaque assays. Small plaques of about 1 mm in radius were formed in BGM cells. Large plaques of about 3–4 mm in radius were consistently produced in monolayers of secondary calf kidney cultures.
G, Butchaiah, E, Lund
openaire +3 more sources
A new method for quantitative analysis of M13 bacteriophage by atomic force microscopy
Synthetic and Systems Biotechnology, 2022 Quantitative analysis is essential for virus research, especially in determining the virus titer. The classical method plaque assay is time-consuming, complex, and difficult for the phages that cannot form apparent plaque on the solid medium. In order to
Yuting Wu +6 more
doaj +1 more source
Plaque assay for virulent Legionella pneumophila [PDF]
Journal of Clinical Microbiology, 1989 Methods of assessing virulence of Legionella pneumophila, the etiologic agent of Legionnaires disease, include the infection of guinea pigs, fertile chicken eggs, and mammalian and protozoan cell cultures. Guinea pig assays, in particular, are expensive, laborious, or unsuitable for routine screening of Legionella isolates.
R C, Fernandez +4 more
openaire +2 more sources