Results 51 to 60 of about 152,477 (295)

A new method for quantitative analysis of M13 bacteriophage by atomic force microscopy

Synthetic and Systems Biotechnology, 2022
Quantitative analysis is essential for virus research, especially in determining the virus titer. The classical method plaque assay is time-consuming, complex, and difficult for the phages that cannot form apparent plaque on the solid medium. In order to
Yuting Wu, Shuai Liu, Zhiwei Liu, Bing Liu, Bin Du, Zhaoyang Tong, Jianjie Xu   +6 more
doaj   +1 more source

Comparison of bovine rotavirus strains by plaque assay [PDF]

, 1981
peer reviewedUsing plaque assay on MA 104 cells, four strains of bovine rotavirus were compared: the attenuated American strain NCDV, and three virulent strains isolated in Canada (PQ) or in Belgium (S14 and S 77).
Pastoret, Paul-Pierre, Schwers, Anne, Leroy, Pascal, Dagenais, Lise   +3 more
core   +1 more source

Development of an automated plaque-counting program for the quantification of the Chikungunya virus

Scientific Reports
Chikungunya virus (CHIKV) induces a massive cytopathic effect (CPE) on various cell types. Therefore, the plaque assay, a CPE-based virus titration method, remains the gold standard for quantifying the infectious units of CHIKV.
Akino Emi, Youichi Suzuki, Chinami Odake, Shoichi Sakaguchi, Hong Wu, Takashi Nakano   +5 more
doaj   +1 more source

Infection Counter: Automated Quantification of in Vitro Virus Replication by Fluorescence Microscopy

Viruses, 2016
The ability to accurately and reliably quantify viral infection is essential to basic and translational virology research. Here, we describe a simple and robust automated method for using fluorescence microscopy to estimate the proportion of virally ...
Siân Culley, Greg J. Towers, David L. Selwood, Ricardo Henriques, Joe Grove   +4 more
doaj   +1 more source

Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation

FEBS Letters, EarlyView.
Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz, Mihály Mérey, Rebeka Vásárhelyi, Ramóna Pék, Victor Imburchia, László Henn, Adrián Kószó, Nicholas D. Lakin, Ivan Ahel, Sébastien Huet, Ágnes Czibula, Gyula Timinszky   +11 more
wiley   +1 more source

Salmonella lipopolysaccharide‐containing supported lipid bilayers as platforms to study bacteriophage interactions

FEBS Letters, EarlyView.
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace, Alyssa Borges, Charles Arnaud, Tove Karlsson, Nina K. Bröker, Stefanie Barbirz, Marta Bally   +6 more
wiley   +1 more source

Population dynamics of SNJ1 determined by plaque assay and qPCR.

, 2016
The cells of Natrinema sp. J7-2 were infected by halovirus SNJ1 with an MOI of 50 for 1.5 h, then samples were taken from the culture system at indicated times, and the titers of samples were detected by plaque assay and qPCR.
Ming Yang (109148), Congcong He (719033), Yunjun Mei (719032), Dala Ba (3168687), Shunxi Zhang (3168690), Ping Shen (149355), Wei Deng (17330), Jian Zhang (1682), Xiangdong Chen (149352)   +8 more
core   +1 more source

Real-Time qPCR as a Method for Detection of Antibody-Neutralized Phage Particles

Frontiers in Microbiology, 2017
The most common method for phage quantitation is the plaque assay, which relies on phage ability to infect bacteria. However, non-infective phage particles may preserve other biological properties; specifically, they may enter interactions with the ...
Anna Kłopot, Adriana Zakrzewska, Dorota Lecion, Joanna M. Majewska, Marek A. Harhala, Karolina Lahutta, Zuzanna Kaźmierczak, Łukasz Łaczmański, Łukasz Łaczmański, Marlena Kłak, Krystyna Dąbrowska, Krystyna Dąbrowska   +11 more
doaj   +1 more source

Microbiome−host proteostasis crosstalk—An emerging perspective on mechanisms and interventions toward healthy longevity

FEBS Letters, EarlyView.
Proteostasis and the gut microbiota play a key role in shaping host physiology. Microbiota‐derived metabolites, vitamins, and RNA modulate host proteostasis. Findings from model systems, including C. elegans, indicate microbes can either stabilize or disrupt host proteostasis.
Abhishek Anil Dubey, Maria Ermolaeva
wiley   +1 more source

Estudo da incidência de adenovírus humano e suíno, norovírus humano e circovírus suíno, em amostras de água de consumo e descedentação animal do município de Concórdia, Santa Catarina [PDF]

, 2011
TCC(graduação) - Universidade Federal de Santa Catarina. Centro de Ciências Biológicas. Biologia.Os dejetos provenientes da suinocultura e da população podem potencialmente contaminar mananciais de captação de água para consumo, já que esses efluentes ...
Garcia, Lucas Ariel Totaro
core