Real-Time qPCR as a Method for Detection of Antibody-Neutralized Phage Particles
Frontiers in Microbiology, 2017 The most common method for phage quantitation is the plaque assay, which relies on phage ability to infect bacteria. However, non-infective phage particles may preserve other biological properties; specifically, they may enter interactions with the ...
Anna Kłopot +11 more
doaj +1 more source
The SARS-coronavirus-host interactome [PDF]
, 2011 Coronaviruses (CoVs) are important human and animal pathogens that induce fatal respiratory, gastrointestinal and neurological disease. The outbreak of the severe acute respiratory syndrome (SARS) in 2002/2003 has demonstrated human vulnerability to ...
Brunn, Albrecht von +27 more
core +1 more source
Rapid Plaque Assay for Encephalomyocarditis Virus [PDF]
Applied Microbiology, 1969 A liquid overlay plaquing technique is described which offers a rapid and simple plaque assay system for small plaque variants of encephalomyocarditis virus.
D A, Baker, L A, Glasgow
openaire +2 more sources
Development of an automated plaque-counting program for the quantification of the Chikungunya virus
Scientific ReportsChikungunya virus (CHIKV) induces a massive cytopathic effect (CPE) on various cell types. Therefore, the plaque assay, a CPE-based virus titration method, remains the gold standard for quantifying the infectious units of CHIKV.
Akino Emi +5 more
doaj +1 more source
Viruses, 2019 Genetic variation in baculoviruses is recognized as a key factor, not only due to the influence of such variation on pathogen transmission and virulence traits, but also because genetic variants can form the basis for novel biological insecticides.
Eduardo Aguirre +3 more
doaj +1 more source
Analysis of interspecies adherence of oral bacteria using a membrane binding assay coupled with polymerase chain reaction-denaturing gradient gel electrophoresis profiling. [PDF]
, 2011 Information on co-adherence of different oral bacterial species is important for understanding interspecies interactions within oral microbial community.
He, Xue-song +6 more
core
Optimization of quantitative polymerase chain reactions for detection and quantification of eight periodontal bacterial pathogens [PDF]
, 2012 BACKGROUND: The aim of this study was to optimize quantitative (real-time) polymerase chain reaction (qPCR) assays for 8 major periodontal pathogens, i.e.
Cosyn, Jan +8 more
core +1 more source
Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation
FEBS Letters, EarlyView.Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz +11 more
wiley +1 more source
Plaque Assay Of Newcastle Disease Virus [PDF]
, 1989 The Newcastle disease virus (NDV) was isolated from a 3 months-old indigenous chicken (buras or kampung chicken) which showed clinical signs of Newcastle disease (ND).
Sardjono, B. (B)
core
Vector competence of Aedes aegypti, Culex tarsalis, and Culex quinquefasciatus from California for Zika virus. [PDF]
, 2018 Zika virus (ZIKV) has emerged since 2013 as a significant global human health threat following outbreaks in the Pacific Islands and rapid spread throughout South and Central America.
Barker, Christopher M +9 more
core +3 more sources