Results 31 to 40 of about 1,087,535 (239)

Development of an automated plaque-counting program for the quantification of the Chikungunya virus

Scientific Reports
Chikungunya virus (CHIKV) induces a massive cytopathic effect (CPE) on various cell types. Therefore, the plaque assay, a CPE-based virus titration method, remains the gold standard for quantifying the infectious units of CHIKV.
Akino Emi, Youichi Suzuki, Chinami Odake, Shoichi Sakaguchi, Hong Wu, Takashi Nakano   +5 more
doaj   +1 more source

A new method for quantitative analysis of M13 bacteriophage by atomic force microscopy

Synthetic and Systems Biotechnology, 2022
Quantitative analysis is essential for virus research, especially in determining the virus titer. The classical method plaque assay is time-consuming, complex, and difficult for the phages that cannot form apparent plaque on the solid medium. In order to
Yuting Wu, Shuai Liu, Zhiwei Liu, Bing Liu, Bin Du, Zhaoyang Tong, Jianjie Xu   +6 more
doaj   +1 more source

Microbiome−host proteostasis crosstalk—An emerging perspective on mechanisms and interventions toward healthy longevity

FEBS Letters, EarlyView.
Proteostasis and the gut microbiota play a key role in shaping host physiology. Microbiota‐derived metabolites, vitamins, and RNA modulate host proteostasis. Findings from model systems, including C. elegans, indicate microbes can either stabilize or disrupt host proteostasis.
Abhishek Anil Dubey, Maria Ermolaeva
wiley   +1 more source

Infection Counter: Automated Quantification of in Vitro Virus Replication by Fluorescence Microscopy

Viruses, 2016
The ability to accurately and reliably quantify viral infection is essential to basic and translational virology research. Here, we describe a simple and robust automated method for using fluorescence microscopy to estimate the proportion of virally ...
Siân Culley, Greg J. Towers, David L. Selwood, Ricardo Henriques, Joe Grove   +4 more
doaj   +1 more source

Ultra-short echo time cardiovascular magnetic resonance of atherosclerotic carotid plaque [PDF]

, 2010
Background Multi-contrast weighted cardiovascular magnetic resonance (CMR) allows detailed plaque characterisation and assessment of plaque vulnerability.
Sonia Nielles-Vallespin, Nielles-Vallespin, S, Firmin, DN, Chan, Cheuk F, Keenan, Niall G, Mary N Sheppard, Gatehouse, Peter, Chan Cheuk F, Firmin David N, Gatehouse, P, Keenan, NG, Sheppard, MN, Boyle, JJ, Peter Gatehouse, Chan, CF, Sheppard Mary N, Boyle Joseph J, Joseph J Boyle, Niall G Keenan, Nielles-Vallespin, Sonia, Boyle, Joseph J, Sheppard, Mary N, Pennell, Dudley J, Pennell, DJ, Pennell Dudley J, Gatehouse Peter, Dudley J Pennell, Nielles-Vallespin Sonia, David N Firmin, Cheuk F Chan, Keenan Niall G, Firmin, David N   +31 more
core   +1 more source

Peripheral lysosomes recruit PLEKHG3 to focal adhesions and restrain protrusion dynamics

FEBS Letters, EarlyView.
Proximity‐dependent labeling at the LAMTOR complex revealed the Rho GEF PLEKHG3 as a lysosome‐proximal protein directing the study toward the influence of lysosome positioning on actin dynamics and cell motility. We show that PLEKHG3 colocalizes with lysosomes at focal adhesion sites and observe that forced peripheral dispersion of lysosomes hinders ...
Rainer Ettelt, Ana‐Maria Sandru, Georg Vucak, Sebastian Didusch, Biljana Riemelmoser, Karin Ehrenreiter, Markus Hartl, Lukas A. Huber, Manuela Baccarini   +8 more
wiley   +1 more source

Engineering peptides into antibodies—opportunities and strategies for therapeutic innovation

FEBS Letters, EarlyView.
Peptides and antibodies occupy complementary therapeutic niches. Peptides recognize difficult targets in a compact format, while antibodies add specificity, long half‐life, and effector functions. This review examines strategies that merge both modalities—peptide grafting into loops, terminal and Fc fusions, and bioconjugation—highlighting how ...
Jinling Wang, Ruslana Romaniuk, Hristo L. Svilenov   +2 more
wiley   +1 more source

Real-Time qPCR as a Method for Detection of Antibody-Neutralized Phage Particles

Frontiers in Microbiology, 2017
The most common method for phage quantitation is the plaque assay, which relies on phage ability to infect bacteria. However, non-infective phage particles may preserve other biological properties; specifically, they may enter interactions with the ...
Anna Kłopot, Adriana Zakrzewska, Dorota Lecion, Joanna M. Majewska, Marek A. Harhala, Karolina Lahutta, Zuzanna Kaźmierczak, Łukasz Łaczmański, Łukasz Łaczmański, Marlena Kłak, Krystyna Dąbrowska, Krystyna Dąbrowska   +11 more
doaj   +1 more source

Treatment with KCL‐286, a first‐in‐class retinoic acid receptor‐β (RARβ) agonist, ameliorates neuronal DNA damage and inflammation in a mouse model of Alzheimer's disease

FEBS Open Bio, EarlyView.
Repair of neuronal DNA damage in Alzheimer's disease by KCL‐286. (A) Amyloid‐β oligomers and plaques impair neuronal DNA repair pathways, leading to DNA double‐strand breaks and glial activation. (B) KCL‐286 activates RARβ/RXR signalling via retinoic acid response elements (RAREs), associated with increased BRCA1 expression, enhanced DNA repair and ...
Natasha Hill, Hassan Yousef Othman AlMuallim, Elouisa Maddock, Carl Hobbs, Earl Clarke, Maria B. Goncalves, Jonathan P. T. Corcoran   +6 more
wiley   +1 more source

Genetic Variability of Chrysodeixis Includens Nucleopolyhedrovirus (ChinNPV) and the Insecticidal Characteristics of Selected Genotypic Variants

Viruses, 2019
Genetic variation in baculoviruses is recognized as a key factor, not only due to the influence of such variation on pathogen transmission and virulence traits, but also because genetic variants can form the basis for novel biological insecticides.
Eduardo Aguirre, Inés Beperet, Trevor Williams, Primitivo Caballero   +3 more
doaj   +1 more source