Standardization of Quantitative Plaque-Based Viral Assays for Orthoflavivirus Cacipacoré
VirusesCacipacoré virus (CPCV) is an understudied orthoflavivirus with significant gaps in research that hinders our understanding of its ecology, host range, and potential public health impact. A notable barrier to filling this gap is the absence of standardized methodologies for viral quantification, such as plaque-forming and focus-forming assays.
Marielena Vogel Saivish +6 more
openaire +2 more sources
Rapid, High-Level Transient Expression of Papillomavirus-Like Particles in Insect Cells
BioTechniques, 1996 Empirical scanning of natural or engineered peptide sequences for functional residues is inherently dependent upon efficient expression of large numbers of individual sequence variants to assay their relative functional potency.
Diana Benincasa +3 more
doaj +1 more source
Zika virus isolation, propagation, and quantification using multiple methods.
PLoS ONE, 2021 Zika virus (ZIKV) was isolated from the archival urine, serum, and autopsy specimens by intrathoracic inoculation of Toxorhynchitis splendens and followed by three blind sub-passaging in C6/36 mosquito cells.
Worawat Dangsagul +8 more
doaj +1 more source
Development of a robust cell-based potency assay for a coxsackievirus A21 oncolytic virotherapy
HeliyonOncolytic viruses (OV) are part of a burgeoning field of investigational oncolytic therapy (OT), in which lytic viruses dissolve advanced tumors productively and specifically.
Venkateswarlu Chamcha +6 more
doaj +1 more source
Fluorescence Adherence Inhibition Assay: A Novel Functional Assessment of Blocking Virus Attachment by Vaccine-Induced Antibodies. [PDF]
PLoS ONE, 2016 Neutralizing antibodies induced by vaccination or natural infection play a critically important role in protection against the viral diseases. In general, neutralization of the viral infection occurs via two major pathways: pre- and post-attachment modes,
Atul Asati +9 more
doaj +1 more source
Effects of US7 and UL56 on Cell-to-Cell Spread of Human Herpes Simplex Virus 1
Viruses, 2023 Human herpes simplex virus (HSV), a double-stranded DNA virus belonging to the Herpesviridae family and alpha herpesvirus subfamily, is one of the most epidemic pathogens in the population.
Jun Wang +10 more
doaj +1 more source
A guinea pig model of Zika virus infection
Virology Journal, 2017 Background Animal models are critical to understand disease and to develop countermeasures for the ongoing epidemic of Zika virus (ZIKV). Here we report that immunocompetent guinea pigs are susceptible to infection by a contemporary American strain of ...
Mukesh Kumar +4 more
doaj +1 more source
Real-time PCR provides improved detection and titer determination of bacteriophage
BioTechniques, 2003 The plaque assay is the traditional method for the quantification of bacteriophage, particularly for λ cloning vectors. Unfortunately, this technique is fraught with procedural difficulties, and the quality of the data obtained from this “gold standard ...
Daniel C. Edelman, Janet Barletta
doaj +1 more source
Quantification of Infectious Sendai Virus Using Plaque Assay
Bio-Protocol, 2018 Sendai virus (SeV) is an enveloped, single-stranded RNA virus of the family Paramyxoviridae. SeV is a useful tool to study its infectious pathomechanism in immunology and the pathomechanism of a murine model of IgA nephropathy.
Narihito Tatsumoto +3 more
doaj +1 more source
PLoS ONE, 2019 Preventive viral vaccine efficacy trials require large-scale sample analysis to quantitate immune responses and their correlation with infection outcomes.
Tamara P Blevins +4 more
doaj +1 more source