Results 31 to 40 of about 84,952 (272)

In Vitro Viral Recovery Yields under Different Re-Suspension Buffers in Iron Flocculation to Concentrate Viral Hemorrhagic Septicemia Virus Genotype IVa in Seawater

Animals, 2023
Iron flocculation is widely used to concentrate viruses in water, followed by Fe-virus flocculate formation, collection, and elution. In the elution stage, an oxalic or ascorbic acid re-suspension buffer dissolved iron hydroxide.
Na-Gyeong Ryu, Eun-Jin Baek, Min-Jae Kim, Kwang-Il Kim   +3 more
doaj   +1 more source

Infectious Focus Assays and Multiplicity of Infection (MOI) Calculations for Alpha-herpesviruses

Bio-Protocol, 2014
Titration of viral stocks is a critical process before any experimental use of the virus. Here we describe an infectious focus assay for several alphaherpesviruses, a titration method for fluorescently labeled viruses, based on the original plaque assay.
Anna Sloutskin, Ronald Goldstein
doaj   +1 more source

Suramin Inhibits Chikungunya Virus Entry and Transmission. [PDF]

PLoS ONE, 2015
The mosquito-borne Chikungunya virus (CHIKV) is a profound global threat due to its high rate of contagion and the lack of vaccine or effective treatment.
Yi-Jung Ho, Yu-Ming Wang, Jeng-wei Lu, Tzong-Yuan Wu, Liang-In Lin, Szu-Cheng Kuo, Chang-Chi Lin   +6 more
doaj   +1 more source

The Viral Replication Complex Is Associated with the Virulence of Newcastle Disease Virus

, 2010
Virulent strains of Newcastle disease virus ([NDV] also known as avian paramyxovirus type 1) can be discriminated from low-virulence strains by the presence of multiple basic amino acid residues at the proteolytic cleavage site of the fusion (F) protein.
Peeters, B.P.H., G. Koch, B. P. H. Peeters, Rottier, P.J.M., Dortmans, J.C.F.M., J. C. F. M. Dortmans, P. J. M. Rottier, Koch, G.   +7 more
core   +1 more source

Reliability of medical students' vaccination histories for immunisable diseases [PDF]

, 2008
Background Medical students come into contact with infectious diseases early on their career. Immunity against vaccine-preventable diseases is therefore vital for both medical students and the patients with whom they come into contact.
René Gottschalk, Allwinn Regina, Allwinn, Regina, Gottschalk, René, Sabine Wicker, Regina Allwinn, Rabenau, Holger, Holger F Rabenau, Gottschalk René, Rabenau Holger F, Wicker, Sabine, Wicker Sabine   +11 more
core   +1 more source

Population dynamics of SNJ1 determined by plaque assay and qPCR.

, 2016
The cells of Natrinema sp. J7-2 were infected by halovirus SNJ1 with an MOI of 50 for 1.5 h, then samples were taken from the culture system at indicated times, and the titers of samples were detected by plaque assay and qPCR.
Ming Yang (109148), Congcong He (719033), Yunjun Mei (719032), Dala Ba (3168687), Shunxi Zhang (3168690), Ping Shen (149355), Wei Deng (17330), Jian Zhang (1682), Xiangdong Chen (149352)   +8 more
core   +1 more source

Rapid, High-Level Transient Expression of Papillomavirus-Like Particles in Insect Cells

BioTechniques, 1996
Empirical scanning of natural or engineered peptide sequences for functional residues is inherently dependent upon efficient expression of large numbers of individual sequence variants to assay their relative functional potency.
Diana Benincasa, Melvin Silberklang, George E. Mark, Steven W. Ludmerer   +3 more
doaj   +1 more source

Zika virus isolation, propagation, and quantification using multiple methods.

PLoS ONE, 2021
Zika virus (ZIKV) was isolated from the archival urine, serum, and autopsy specimens by intrathoracic inoculation of Toxorhynchitis splendens and followed by three blind sub-passaging in C6/36 mosquito cells.
Worawat Dangsagul, Kriengsak Ruchusatsawat, Apiwat Tawatsin, Don Changsom, Pirom Noisumdaeng, Sukontip Putchakarn, Chayawat Phatihattakorn, Prasert Auewarakul, Pilaipan Puthavathana   +8 more
doaj   +1 more source

Passaging of a Newcastle disease virus pigeon variant in chickens results in selection of viruses with mutations in the polymerase complex enhancing virus replication and virulence

, 2010
Some Newcastle disease virus (NDV) variants isolated from pigeons (pigeon paramyxovirus type 1; PPMV-1) do not show their full virulence potential for domestic chickens but may become virulent upon spread in these animals.
Peeters, B.P.H., Strategic Infection Biology, Dep Infectieziekten Immunologie, G. Koch, B. P. H. Peeters, Rottier, P.J.M., Dortmans, J.C.F.M., J. C. F. M. Dortmans, P. J. M. Rottier, Koch, G.   +9 more
core   +1 more source

Rapid and stain-free quantification of viral plaque via lens-free holography and deep learning

, 2023
We present a rapid and stain-free quantitative viral plaque assay using lensfree holographic imaging and deep learning. This cost-effective, compact, and automated device significantly reduces the incubation time needed for traditional plaque assays ...
Zhang, Yijie, Ozcan, Aydogan, Li, Jingxi, Bijie Bai, Aydogan Ozcan, Liu, Tairan, Yijie Zhang, Hongda Wang, Jingxi Li, Li, Yuzhu, Ma, Guangdong, Merve Eryilmaz, Wang, Hongda, Guangdong Ma, Yuzhu Li, Koydemir, Hatice, Eryilmaz, Merve, Hatice Ceylan Koydemir, Koydemir, Hatice Ceylan, Yang, Ethan, Tairan Liu, Bai, Bijie, Ethan Yang   +22 more
core   +1 more source