Results 11 to 20 of about 82,571 (135)
Variables affecting viral plaque formation in microculture plaque assays using homologous antibody in a liquid overlay [PDF]
Journal of Clinical Microbiology, 1977 A liquid antibody microculture plaque assay and the variables that govern its effectiveness are described. The assay is based on the principle that low concentrations of homologous antibody can inhibit secondary plaque formation without inhibiting formation of primary plaques. Thus, clear plaques that followed a linear dose response were produced.
A S, Randhawa +3 more
openaire +2 more sources
Viral Concentration Determination Through Plaque Assays: Using Traditional and Novel Overlay Systems [PDF]
Journal of Visualized Experiments, 2014 Plaque assays remain one of the most accurate methods for the direct quantification of infectious virons and antiviral substances through the counting of discrete plaques (infectious units and cellular dead zones) in cell culture. Here we demonstrate how to perform a basic plaque assay, and how differing overlays and techniques can affect plaque ...
Alan, Baer, Kylene, Kehn-Hall
openaire +2 more sources
SARS-CoV-2 RBD protein enhances the oncolytic activity of the vesicular stomatitis virus
Frontiers in Immunology, 2023 Despite recent advances in the research on oncolytic viruses (OVs), a better understanding of how to enhance their replication is key to improving their therapeutic index.
Almohanad A. Alkayyal +21 more
doaj +1 more source
Immunoplaque Assay (Influenza Virus)
Bio-Protocol, 2013 Despite developed long time ago, plaque assay is still the gold standard for viral titer quantification in modern virology. The standard crystal violet-based plaque assay relies on virus’ ability to induce cytopathic effect (CPE) which limits the assay ...
Victor Tse +2 more
doaj +1 more source
A Useful Quantitative Semimicromethod for Viral Plaque Assay
Experimental Biology and Medicine, 1973 This paper presents a general method for rapid, easy, and convenient quantitation of virus titers which we have utilized in applications varying from measurement of virus distribution in infection in vivo to assay of neutralizing antibody or antiviral agents.
B, Rager-Zisman, T C, Merigan
openaire +2 more sources
Plaques Formed by Mutagenized Viral Populations Have Elevated Coinfection Frequencies
mBio, 2017 The plaque assay is a common technique used to measure virus concentrations and is based upon the principle that each plaque represents a single infectious unit.
Elizabeth R. Aguilera +4 more
doaj +1 more source
BMC Infectious Diseases, 2020 Background The polymerase chain reaction (PCR) is commonly used to detect viral pathogens because of its high sensitivity and specificity. However, conventional PCR methods cannot determine virus infectivity.
Yuki Nakaya +4 more
doaj +1 more source
Virucidal and Neutralizing Activity Tests for Antiviral Substances and Antibodies
Bio-Protocol, 2018 In a narrow definition, virucidal activity represents the activity by which to interact with and physically disrupt viral particles. In a broad definition, it includes the activity by which to functionally inhibit (neutralize) viral infectivity without ...
Chie Aoki-Utsubo, Ming Chen, Hak Hotta
doaj +1 more source
Iranian Journal of Microbiology, 2022 Background and Objectives: SARS-CoV-2 variants of concern (VOC) and interest (VOI) pose a significant threat to public health because the rapid change in the SARS-CoV-2 genome can alter viral phenotypes such as virulence, transmissibility and the ...
Maria Isabel Zapata-Cardona +8 more
doaj
Peptides corresponding to the predicted heptad repeat 2 domain of the feline coronavirus spike protein are potent inhibitors of viral infection. [PDF]
PLoS ONE, 2013 BACKGROUND: Feline infectious peritonitis (FIP) is a lethal immune-mediated disease caused by feline coronavirus (FCoV). Currently, no therapy with proven efficacy is available.
I-Jung Liu +3 more
doaj +1 more source