Immunoplaque Assay (Influenza Virus)
Bio-Protocol, 2013 Despite developed long time ago, plaque assay is still the gold standard for viral titer quantification in modern virology. The standard crystal violet-based plaque assay relies on virus’ ability to induce cytopathic effect (CPE) which limits the assay ...
Victor Tse +2 more
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, 2022 Background and Objectives: SARS-CoV-2 variants of concern (VOC) and interest (VOI) pose a significant threat to public health because the rapid change in the SARS-CoV-2 genome can alter viral phenotypes such as virulence, transmissibility and the ability
Rugeles, Maria Teresa +8 more
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BMC Infectious Diseases, 2020 Background The polymerase chain reaction (PCR) is commonly used to detect viral pathogens because of its high sensitivity and specificity. However, conventional PCR methods cannot determine virus infectivity.
Yuki Nakaya +4 more
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Effects of Ionic Strength on Bacteriophage MS2 Behavior and Their Implications for the Assessment of Virus Retention by Ultrafiltration Membranes [PDF]
, 2011 Bacteriophage MS2 is widely used as a surrogate to estimate pathogenic virus elimination by membrane filtration processes used in water treatment.
Mathieu Berge +13 more
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Virucidal and Neutralizing Activity Tests for Antiviral Substances and Antibodies
Bio-Protocol, 2018 In a narrow definition, virucidal activity represents the activity by which to interact with and physically disrupt viral particles. In a broad definition, it includes the activity by which to functionally inhibit (neutralize) viral infectivity without ...
Chie Aoki-Utsubo, Ming Chen, Hak Hotta
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Peptides corresponding to the predicted heptad repeat 2 domain of the feline coronavirus spike protein are potent inhibitors of viral infection. [PDF]
PLoS ONE, 2013 BACKGROUND: Feline infectious peritonitis (FIP) is a lethal immune-mediated disease caused by feline coronavirus (FCoV). Currently, no therapy with proven efficacy is available.
I-Jung Liu +3 more
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Generation and characterization of a recombinant Rift Valley fever virus expressing a V5 epitope-tagged RNA-dependent RNA polymerase [PDF]
, 2011 The viral RNA-dependent RNA polymerase (RdRp; L protein) of Rift Valley fever virus (RVFV; family Bunyaviridae) is a 238 kDa protein that is crucial for the life cycle of the virus, as it catalyses both transcription of viral mRNAs and replication of the
Brennan, Benjamin +5 more
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ViralPlaque: a Fiji macro for automated assessment of viral plaque statistics [PDF]
PeerJ, 2019 Plaque assay has been used for a long time to determine infectious titers and characterize prokaryotic and eukaryotic viruses forming plaques. Indeed, plaque morphology and dimensions can provide information regarding the replication kinetics and the ...
Marco Cacciabue +2 more
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Antiviral activity of recombinant porcine interferon-α against porcine transmissible gastroenteritis virus in pk-15 cells [PDF]
Kafkas Universitesi Veteriner Fakültesi Dergisi, 2017 A recombinant porcine interferon alpha (rPoIFN-α) has been developed and patented previously (Chinese patent number ZL200810020180.4). In the current study, we investigated the inhibitory effects of the rPoIFN-α on the propagation of porcine ...
ZHAO J +8 more
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Determination of HSV-1 infectivity by plaque assay and a luciferase reporter cell line
, 2022 S.171-181Quantification of infectious virus is crucial to many experimental approaches in virological research. A broadly used and facile technique is the so-called ""plaque assay"" which provides precise information on the absolute quantity of ...
Bailer, S., Lieber, D.
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