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Dynamic Model Visualizing the Process of Viral Plaque Formation
Journal of Microbiology & Biology Education, 2012 In Microbiology and Virology courses, viral plaques are often presented to students as the way one can visualize viruses/bacteriophages. While students generally grasp the idea that counting plaques is essentially the same as counting viruses in their ...
Boriana Marintcheva
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ViralPlaque: a Fiji macro for automated assessment of viral plaque statistics [PDF]
PeerJ, 2019 Plaque assay has been used for a long time to determine infectious titers and characterize prokaryotic and eukaryotic viruses forming plaques. Indeed, plaque morphology and dimensions can provide information regarding the replication kinetics and the ...
Marco Cacciabue +2 more
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Validation of the Filovirus Plaque Assay for Use in Preclinical Studies
Viruses, 2016 A plaque assay for quantitating filoviruses in virus stocks, prepared viral challenge inocula and samples from research animals has recently been fully characterized and standardized for use across multiple institutions performing Biosafety Level 4 (BSL ...
Amy C. Shurtleff +7 more
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Antiviral activity of recombinant porcine interferon-α against porcine transmissible gastroenteritis virus in pk-15 cells [PDF]
Kafkas Universitesi Veteriner Fakültesi Dergisi, 2017 A recombinant porcine interferon alpha (rPoIFN-α) has been developed and patented previously (Chinese patent number ZL200810020180.4). In the current study, we investigated the inhibitory effects of the rPoIFN-α on the propagation of porcine ...
ZHAO J +8 more
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Animals, 2023 Iron flocculation is widely used to concentrate viruses in water, followed by Fe-virus flocculate formation, collection, and elution. In the elution stage, an oxalic or ascorbic acid re-suspension buffer dissolved iron hydroxide.
Na-Gyeong Ryu +3 more
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Frontiers in Public HealthIntroductionBacteriophage MS2 is widely applied as a surrogate for pathogenic non-enveloped RNA viruses that are difficult to culture. However, standardized approaches for evaluating disinfectant efficacy using MS2 remain limited.
Sung Jae Kim +8 more
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Infectious Focus Assays and Multiplicity of Infection (MOI) Calculations for Alpha-herpesviruses
Bio-Protocol, 2014 Titration of viral stocks is a critical process before any experimental use of the virus. Here we describe an infectious focus assay for several alphaherpesviruses, a titration method for fluorescently labeled viruses, based on the original plaque assay.
Anna Sloutskin, Ronald Goldstein
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Suramin Inhibits Chikungunya Virus Entry and Transmission. [PDF]
PLoS ONE, 2015 The mosquito-borne Chikungunya virus (CHIKV) is a profound global threat due to its high rate of contagion and the lack of vaccine or effective treatment.
Yi-Jung Ho +6 more
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Plaque Assay For Screening Viral Concentrates for Bacteriophage v1 [PDF]
, 2017 Please contact Dr. Steven Wilhelm (wilhelm@utk.edu) for additional information regarding this protocol.
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