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Isolation of Plasma Membrane and Plasma Membrane Microdomains
2016The plasma membrane surrounds the cytoplasm of a cell and functions as a barrier to separate the intracellular compartment from the extracellular environment. Protein and lipid components distribute nonuniformly and the components form clusters with various functions in the plasma membrane.
Anzu, Minami +3 more
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Current Opinion in Cell Biology, 2002
Several lines of evidence indicate that the lipids in the plasma membrane of animal cells are inhomogeneously distributed, and that various types of specialized lipid domains play an important role in many biological processes. The characteristics of these domains, such as size, composition and dynamics, are currently under active investigation.
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Several lines of evidence indicate that the lipids in the plasma membrane of animal cells are inhomogeneously distributed, and that various types of specialized lipid domains play an important role in many biological processes. The characteristics of these domains, such as size, composition and dynamics, are currently under active investigation.
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2002
Publisher Summary This chapter discusses the biogenesis of plasma membranes. A consistent theme at the plasma membrane is protein recruitment and the assembly of protein complexes in response to external stimuli. The growth of the plasma membrane is localized to the bud site during normal cell growth and to the projection in response to mating ...
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Publisher Summary This chapter discusses the biogenesis of plasma membranes. A consistent theme at the plasma membrane is protein recruitment and the assembly of protein complexes in response to external stimuli. The growth of the plasma membrane is localized to the bud site during normal cell growth and to the projection in response to mating ...
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Plasma membrane: Negative attraction
Nature Plants, 2016The electrostatic charge at the inner surface of the plasma membrane is strongly negative in higher organisms. A new study shows that phosphatidylinositol-4-phosphate plays a critical role in establishing plasma membrane surface charge in Arabidopsis, which regulates the correct localization of signalling components.
Gergely, Molnár +2 more
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Mammalian plasma membrane proteomics
PROTEOMICS, 2007Abstract Plasma membrane proteins serve essential functions for cells, interacting with both cellular and extracellular components, structures and signaling molecules. Additionally, plasma membrane proteins comprise more than two‐thirds of the known protein targets for existing drugs.
Josic, D, Clifton, JG
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Cytoskeleton—Plasma Membrane Interactions
Science, 1992Proteins at the boundary between the cytoskeleton and the plasma membrane control cell shape, delimit specialized membrane domains, and stabilize attachments to other cells and to the substrate. These proteins also regulate cell locomotion and cytoplasmic responses to growth factors and other external stimuli.
Luna, Elizabeth J., Hitt, Anne L.
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Biochemical Society Transactions, 2006
IP3Rs (inositol 1,4,5-trisphosphate receptors) are expressed in the membranes of non-mitochondrial organelles in most animal cells, but their presence and role within the plasma membrane are unclear. Whole-cell patch–clamp recording from DT40 cells expressing native or mutated IP3Rs has established that each cell expresses just two or three functional ...
C W, Taylor, O, Dellis
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IP3Rs (inositol 1,4,5-trisphosphate receptors) are expressed in the membranes of non-mitochondrial organelles in most animal cells, but their presence and role within the plasma membrane are unclear. Whole-cell patch–clamp recording from DT40 cells expressing native or mutated IP3Rs has established that each cell expresses just two or three functional ...
C W, Taylor, O, Dellis
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Sperm plasma membrane receptors for the porcine oocyte plasma membrane
Zygote, 1998In vitro fertilisation (IVF) was used to assess the ability of solubilised sperm plasma membrane (PM) proteins to inhibit the interaction of intact gametes. This is a first step before evaluating the ability of individual isolated proteins to competitively inhibit sperm-oocyte interaction as part of the process of studying the molecular events of ...
M, Betancourt +5 more
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Cellular plasma membrane domains
Molecular Membrane Biology, 1995The plasma membranes of migrating cells differentiate into at least three distinct domains as defined by the laser tweezers and the motile behaviour of particles bound to specific membrane glycoproteins. These domains are important for steps in the cell migration process.
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