Results 151 to 160 of about 1,390,499 (412)

Plasmid copy number underlies adaptive mutability in bacteria. [PDF]

, 2014
The origin of mutations under selection has been intensively studied using the Cairns-Foster system, in which cells of an Escherichia coli lac mutant are plated on lactose and give rise to 100 Lac+ revertants over several days. These revertants have been
Aboubechara, John Paul, Maisnier-Patin, Sophie, Quiñones-Soto, Semarhy, Roth, John R, Sano, Emiko   +4 more
core   +1 more source

Within-host bacterial growth dynamics with both mutation and horizontal gene transfer [PDF]

arXiv, 2020
The evolution and emergence of antibiotic resistance is a major public health concern. The understanding of the within-host microbial dynamics combining mutational processes, horizontal gene transfer and resource consumption, is one of the keys to solve this problem.
arxiv  

FAM136A depletion induces mitochondrial stress and reduces mitochondrial membrane potential and ATP production

FEBS Open Bio, EarlyView.
FAM136A depletion upregulated ROS production, reduced mitochondrial membrane potential (ΔΨ) and ATP production, and upregulated expression of PCK1, PCK2, HMGCS1, and HMGCS2. The expression of both TOMM22 and TOMM20 was also upregulated. FAM136A depletion reduced HCCS that produce holocytochrome c by combining heme to apocytochrome c, and reduced the ...
Yushi Otsuka, Masato Yano
wiley   +1 more source

Electromediated formation of DNA complexes with cell membranes and its consequences for gene delivery [PDF]

arXiv, 2011
Electroporation is a physical method to induce the uptake of therapeutic drugs and DNA, by eukaryotic cells and tissues. The phenomena behind electro-mediated membrane permeabilization to plasmid DNA have been shown to be significantly more complex than those for small molecules.
arxiv  

Differential plasmid rescue from transgenic mouse DNAs into Escherichia coli methylation-restriction mutants.

Proceedings of the National Academy of Sciences of the United States of America, 1990
Plasmids comprising transgene insertions in four lines of transgenic mice have been retrieved by plasmid rescue into a set of Escherichia coli strains with mutations in different members of the methylation-dependent restriction system (MDRS). Statistical
S. Grant, J. Jessee, F. Bloom, D. Hanahan   +3 more
semanticscholar   +1 more source

Mapping Hsp104 interactions using cross‐linking mass spectrometry

FEBS Open Bio, EarlyView.
This study examines how cross‐linking mass spectrometry can be utilized to analyze ATP‐induced conformational changes in Hsp104 and its interactions with substrates. We developed an analytical pipeline to distinguish between intra‐ and inter‐subunit contacts within the hexameric homo‐oligomer and discovered contacts between Hsp104 and a selected ...
Kinga Westphal, Karolina Michalska, Andrzej Joachimiak, Lukasz A. Joachimiak   +3 more
wiley   +1 more source

Organization of aerobactin, hemolysin, and antibacterial resistance genes in lactose negative Escherichia coli strains of serotype O4 isolated from children with dhiarrea [PDF]

, 1992
Epidemiologically related, non-lactose-fermenting (NLF) Escherichia coli strains of serotype O4 have been isolated at a high frequency from children with diarrhea in Somalia (M. Nicoletti, F. Superti, C. Conti, A. Calconi, and C. Zagaglia, J.
Calconi, Attilio, Casalino, M., Colonna, Bianca, Fradiani, PIERA ASSUNTA, Nicoletti, M., Ranucci, L.   +5 more
core   +1 more source

Bacteriophages limit the existence conditions for conjugative plasmids [PDF]

, 2015
Bacteriophages are a major cause of bacterial mortality and impose strong selection on natural bacterial populations, yet their effects on the dynamics of conjugative plasmids have rarely been tested.
Brockhurst, Michael A., Dytham, Calvin, Harrison, Ellie, Paterson, Steve, Pitchford, Jonathan W., Spiers, Andrew J., Truman, Julie, Wood, Jamie A.   +7 more
core   +5 more sources

Plasmid-Mediated Quinolone Resistance: a Multifaceted Threat

Clinical Microbiology Reviews, 2009
SUMMARY Although plasmid-mediated quinolone resistance (PMQR) was thought not to exist before its discovery in 1998, the past decade has seen an explosion of research characterizing this phenomenon.
J. Strahilevitz, G. Jacoby, D. Hooper, A. Robicsek   +3 more
semanticscholar   +1 more source

Alkane biosynthesis gene expression and its increased production in recombinant cyanobacteria

FEBS Open Bio, EarlyView.
The transcription and mRNA structures of the alkane biosynthesis genes ado/aar were analysed in the representative cyanobacteria PCC 6803 and SK1‐2‐1, which produce C17H36 and C15H32, respectively. PCC 6803 transconjugants carrying these genes showed a 1.8‐ to 2.3‐fold increase in C17H36 production.
Misato Nagao, Takato Ozaki, Hirofumi Fukuda, Yu Kanesaki, Munehiko Asayama   +4 more
wiley   +1 more source