Results 11 to 20 of about 1,390,499 (412)
Native Plasmid-Encoded Mercury Resistance Genes Are Functional and Demonstrate Natural Transformation in Environmental Bacterial Isolates [PDF]
mSystems, 2019 Plasmid-mediated horizontal gene transfer (HGT) is a major driver of genetic diversity in bacteria. We experimentally validated the function of a putative mercury resistance operon present on an abundant 8-kbp native plasmid found in groundwater samples ...
Ankita Kothari +5 more
doaj +4 more sources
Modeling sRNA-regulated Plasmid Maintenance [PDF]
, 2016 We study a theoretical model for the toxin-antitoxin (hok/sok) mechanism for plasmid maintenance in bacteria. Toxin-antitoxin systems enforce the maintenance of a plasmid through post-segregational killing of cells that have lost the plasmid. Key to their function is the tight regulation of expression of a protein toxin by an sRNA antitoxin.
Gong, Chen Chris, Klumpp, Stefan
arxiv +4 more sources
Identification of novel clostridium perfringens type E strains that carry an iota toxin plasmid with a functional enterotoxin gene [PDF]
, 2011 Clostridium perfringens enterotoxin (CPE) is a major virulence factor for human gastrointestinal diseases, such as food poisoning and antibiotic associated diarrhea. The CPE-encoding gene (cpe) can be chromosomal or plasmid-borne.
A Deguchi +32 more
core +16 more sources
Plasmid-to-plasmid recombination in Haemophilus influenzae [PDF]
Journal of Bacteriology, 1986 No recombination between plasmids was observed after conjugal transfer of a plasmid into a cell carrying another plasmid. Two types of such recombination took place after transformation, one type being Rec+ dependent and suggesting a preferred site of recombination. The other much rarer type was at least partially Rec+ independent.
Jane K. Setlow, Meenakshi Balganesh
openaire +3 more sources
Microbiology Spectrum, 2023 Mobile colistin resistance (mcr) genes are present mainly in plasmids and can disseminate clonally or horizontally via either plasmids or insertion sequences in different genomic locations among the Enterobacteriaceae.
Tingting Yang +13 more
doaj +1 more source
Microbiology Spectrum, 2023 During a 2020 routine epidemiological investigation of carbapenem-resistant Enterobacterales at a local food market in Guangzhou, China, two Escherichia coli ST410 isolates coproducing NDM-5 and OXA-181 were obtained from environmental samples ...
Wan-Yun He +7 more
doaj +1 more source
DNA Minicircle Technology Improves Purity of Adeno-associated Viral Vector Preparations
Molecular Therapy: Nucleic Acids, 2016 Adeno-associated viral (AAV) vectors are considered as one of the most promising delivery systems in human gene therapy. In addition, AAV vectors are frequently applied tools in preclinical and basic research. Despite this success, manufacturing pure AAV
Maria Schnödt +9 more
doaj +1 more source
Microbiology Spectrum, 2022 Horizontal gene transfer (HGT) plays a significant role in the spread of antibiotic resistance genes (ARGs). Most reported compounds promote HGT by increasing the cell membrane permeability.
Xia Xiao +4 more
doaj +1 more source
A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
Nucleic Acids Research, 1979 A procedure for extracting plasmid DNA from bacterial cells is described. The method is simple enough to permit the analysis by gel electrophoresis of 100 or more clones per day yet yields plasmid DNA which is pure enough to be digestible by restriction ...
H. Birnboim, J. Doly
semanticscholar +1 more source
Remarkable stability of an instability-prone lentiviral vector plasmid in Escherichia coli Stbl3 [PDF]
, 2013 Large-scale production of plasmid DNA to prepare therapeutic gene vectors or DNA-based vaccines requires a suitable bacterial host, which can stably maintain the plasmid DNA during industrial cultivation.
Al-Allaf, FA +4 more
core +1 more source