Results 41 to 50 of about 1,390,499 (412)

Inference of plasmid copy number mean and noise from single cell gene expression data [PDF]

open access: yesPhys. Rev. E 82, 051916 (2010), 2010
Plasmids are extra-chromosomal DNA molecules which code for their own replication. We previously reported a setup using genes coding for fluorescent proteins of two colors that allowed us, using a simple model, to extract the plasmid copy number noise in a monoclonal population of bacteria [J. Wong Ng et al., Phys. Rev. E, 81, 011909 (2010)].
arxiv   +1 more source

Construction of a novel vector for the nuclear transformation of the unicellular green alga Chlamydomonas reinhardtii and its stable expression

open access: yesJournal of Taibah University for Science, 2019
Plasmid pBI221aadAGUS which carried both of GUS (β-glucuronidase) and aadA (aminoglyoside transferase) genes besides of the 35S cauliflower mosaic virus promoter was constructed and used for stable nuclear transformation of Chlamydomonas reinhardtii. The
Mostafa M. EL-Sheekh   +2 more
doaj   +1 more source

Directed Expression of Tracheal Antimicrobial Peptide as a Treatment for Bovine-Associated Staphylococcus Aureus-Induced Mastitis in Mice

open access: yesFrontiers in Veterinary Science, 2021
Bovine mastitis is perplexing the dairy industry since the initiation of intensive dairy farming, which has caused a reduction in the productivity of cows and an escalation in costs.
Zhipeng Zhang   +13 more
doaj   +1 more source

Noise enhanced persistence in a biochemical regulatory network with feedback control [PDF]

open access: yesPhys. Rev. Lett. 100, 058105 (2008), 2007
We find that discrete noise of inhibiting (signal) molecules can greatly delay the extinction of plasmids in a plasmid replication system: a prototypical biochemical regulatory network. We calculate the probability distribution of the metastable state of the plasmids and show on this example that the reaction rate equations may fail in predicting the ...
arxiv   +1 more source

Plasmids in Rhizobia: The Role of Nonsymbiotic Plasmids

open access: yesMolecular Plant-Microbe Interactions, 1996
Peer ...
Mercado-Blanco, Jesús, Toro, Nicolás
openaire   +3 more sources

Antiparallel plasmid-plasmid pairing may control P1 plasmid replication. [PDF]

open access: yesProceedings of the National Academy of Sciences, 1991
The copy number of the P1 plasmid replicon is stringently controlled, giving only one or two copies per newborn cell. Control is achieved by the action of the copy-control locus incA, which contains nine repeats of the 19-basepair binding site for the plasmid-encoded initiator protein RepA.
Ann L. Abeles, Stuart Austin
openaire   +3 more sources

A Comparison of Plasmid DNA and mRNA as Vaccine Technologies

open access: yesVaccines, 2019
This review provides a comparison of the theoretical issues and experimental findings for plasmid DNA and mRNA vaccine technologies. While both have been under development since the 1990s, in recent years, significant excitement has turned to mRNA ...
Margaret A. Liu
semanticscholar   +1 more source

Predictive Modeling of Non-Viral Gene Transfer [PDF]

open access: yesBiotechnology and Bioengineering, Volume 105, Issue 4, 805-813, (2010), 2010
In non-viral gene delivery, the variance of transgenic expression stems from the low number of plasmids successfully transferred. Here, we experimentally determine Lipofectamine- and PEI-mediated exogenous gene expression distributions from single cell time-lapse analysis.
arxiv   +1 more source

Novel plasmid-mediated colistin resistance gene mcr-7.1 in Klebsiella pneumoniae

open access: yesJournal of Antimicrobial Chemotherapy, 2018
Objectives To identify a novel plasmid-mediated colistin resistance gene in Klebsiella pneumoniae isolated from chickens in China. Methods WGS was used to identify a novel colistin resistance gene.
Yongqiang Yang   +4 more
semanticscholar   +1 more source

Evidence that the 36kb plasmid of Brachyspira hyodysenteriae contributes to virulence [PDF]

open access: yes, 2011
Swine dysentery (SD) results from infection of the porcine large intestine with the anaerobic intestinal spirochaete Brachyspira hyodysenteriae. Recently the genome of virulent Australian B. hyodysenteriae strain WA1 was sequenced, and a 36. kilobase (kb)
Bellgard, M.I.   +5 more
core   +2 more sources

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