Results 261 to 270 of about 982,599 (302)
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Angewandte Chemie, 2017
AbstractDNA‐Hydrogele sind aufgrund ihrer Biokompatibilität und ihrer biologischen Abbaubarkeit für verschiedene biomedizinische Anwendungen von großem Interesse. Bisher werden DNA‐Hydrogele aus synthetischen Bausteinen hergestellt, was mit hohen Kosten verbunden ist.
Tanja Nöll +3 more
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AbstractDNA‐Hydrogele sind aufgrund ihrer Biokompatibilität und ihrer biologischen Abbaubarkeit für verschiedene biomedizinische Anwendungen von großem Interesse. Bisher werden DNA‐Hydrogele aus synthetischen Bausteinen hergestellt, was mit hohen Kosten verbunden ist.
Tanja Nöll +3 more
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Current Protocols in Protein Science, 1998
AbstractThis appendix presents basic procedures for alkaline lysis minipreps, both in tubes and in microtiter plates, and also provides an for boiling lysis. A describes storage of plasmid DNA.
J, Engebrecht, R, Brent
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AbstractThis appendix presents basic procedures for alkaline lysis minipreps, both in tubes and in microtiter plates, and also provides an for boiling lysis. A describes storage of plasmid DNA.
J, Engebrecht, R, Brent
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2002
Substantial progress in medicine and biopharmaceutical research may once enable to cure diseases at the level of the specific gene defects rather than at the conventional phenotype level1,2. In addition, the preventive or curative vaccination against pathogenes like bacteria or viruses3,4,5,67; (review: Ref.
Martin Schleef +3 more
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Substantial progress in medicine and biopharmaceutical research may once enable to cure diseases at the level of the specific gene defects rather than at the conventional phenotype level1,2. In addition, the preventive or curative vaccination against pathogenes like bacteria or viruses3,4,5,67; (review: Ref.
Martin Schleef +3 more
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In vivo plasmid DNA electrotransfer
Current Opinion in Biotechnology, 2002In vivo electrotransfer is a physical technique for gene delivery in various mammalian tissues, which involves the injection of plasmid DNA into a target tissue and administration of an electric field. Its ease of performance, as well as recent understanding of its mechanism and applications to different mammalian tissues such as skeletal muscle, liver,
Pascal, Bigey +2 more
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Current Protocols in Molecular Biology, 1991
AbstractAlthough there are a large number of protocols for the isolation of small quantities of plasmid DNA from bacterial cells (minipreps), this unit presents four procedures based on their speed and success: the alkaline lysis prep, a modification of the alkaline lysis prep that is performed in 1.5‐ml tubes or 96‐well microtiter dishes, the boiling ...
J, Engebrecht, R, Brent, M A, Kaderbhai
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AbstractAlthough there are a large number of protocols for the isolation of small quantities of plasmid DNA from bacterial cells (minipreps), this unit presents four procedures based on their speed and success: the alkaline lysis prep, a modification of the alkaline lysis prep that is performed in 1.5‐ml tubes or 96‐well microtiter dishes, the boiling ...
J, Engebrecht, R, Brent, M A, Kaderbhai
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Microwave Effects on Plasmid DNA
Radiation Research, 1987The exposure of purified plasmid DNA to microwave radiation at nonthermal levels in the frequency range from 2.00 to 8.75 GHz produces single- and double-strand breaks that are detected by agarose gel electrophoresis. Microwave-induced damage to DNA depends on the presence of small amounts of copper.
J L, Sagripanti +2 more
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Insulin Delivery with Plasmid DNA
Human Gene Therapy, 1999Success in controlling hyperglycemia in type I diabetics will require a restoration of basal insulin. To this end, three plasmid DNAs (pDNA) encoding preproinsulin were compared for constitutive expression and processing to insulin in nonendocrine cells in vitro.
A M, Abai, P M, Hobart, K M, Barnhart
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Affinity Adsorption of Plasmid DNA
Biotechnology Progress, 2004The development of a protein-mediated dual functional affinity adsorption of plasmid DNA is described in this work. The affinity ligand for the plasmid DNA comprises a fusion protein with glutathione-S-transferase (GST) as the fusion partner with a zinc finger protein.
Ghose, Siddhartha +2 more
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Rapid screening for plasmid DNA
Molecular and General Genetics MGG, 1977A procedure is described for demonstrating plasmid DNA and its molecular weight, based on rate zonal centrifugation of unlabelled DNA in neutral sucrose gradients containing a low concentration of ethidium bromide. Each DNA species is then visualized as a discrete fluorescent band when the centrifuge tube is illuminated with ultra-violet light ...
C, Hughes, G G, Meynell
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Adjuvants for Plasmid DNA Vaccines
2003In the late 1980s, Jon Wolff of the University of Wisconsin and Phil Felgner here at Vical were screening cationic lipids for their ability to encapsulate and deliver purified plasmid DNA into mouse tissues. They discovered that direct injection of lipid-DNA complexes into muscle resulted in measurable protein expression.
J, Norman +3 more
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