Results 61 to 70 of about 741,058 (313)
Partition of P1 plasmids in Escherichia coli mukB chromosomal partition mutants [PDF]
The partition system of the low-copy-number plasmid/prophage of bacteriophage P1 encodes two proteins, ParA and ParB, and contains a DNA site called parS. ParB and the Escherichia coli protein IHF bind to parS to form the partition complex, in which parS is wrapped around ParB and IHF in a precise three-dimensional conformation.
B E, Funnell, L, Gagnier
openaire +2 more sources
Plant cDNA Similar to a Bacterial Plasmid Partition Locus [PDF]
A, Schaller, J, Schmid, N, Amrhein
openaire +3 more sources
Remarkable stability of an instability-prone lentiviral vector plasmid in Escherichia coli Stbl3 [PDF]
Large-scale production of plasmid DNA to prepare therapeutic gene vectors or DNA-based vaccines requires a suitable bacterial host, which can stably maintain the plasmid DNA during industrial cultivation.
Al-Allaf, FA +4 more
core +1 more source
Partition site of the P1 plasmid. [PDF]
We have defined a minimal partition site, parS, from the plasmid P1. It contains sufficient cis-acting information to direct accurate segregation of low-copy-number plasmids that contain it as long as the two essential P1 Par proteins are supplied in trans. The site is, at most, 34 base pairs and contains a perfect 13-base-pair inverted repeat.
K A, Martin, S A, Friedman, S J, Austin
openaire +2 more sources
The ParB-parS partition complexes that bacterial replicons use to ensure their faithful inheritance also find employment in visualization of DNA loci, as less intrusive alternatives to fluorescent repressor-operator systems. The ability of ParB molecules
Sylvain Audibert +6 more
doaj +1 more source
A bacteriophage tubulin harnesses dynamic instability to center DNA in infected cells. [PDF]
Dynamic instability, polarity, and spatiotemporal organization are hallmarks of the microtubule cytoskeleton that allow formation of complex structures such as the eukaryotic spindle. No similar structure has been identified in prokaryotes.
Agard, David A +6 more
core +2 more sources
Application of nitroarene dioxygenases in the design of novel strains that degrade chloronitrobenzenes. [PDF]
Widespread application of chloronitrobenzenes as feedstocks for the production of industrial chemicals and pharmaceuticals has resulted in extensive environmental contamination with these toxic compounds, where they pose significant risks to the health ...
Ju, Kou-San, Parales, Rebecca E
core +1 more source
Modulation of the P1 Plasmid Partition Protein ParA by ATP, ADP, and P1 ParB*
ParA is an essential P1 plasmid partition protein. It represses transcription of the par genes (parA and parB) and is also required for a second, as yet undefined step in partition.
M. Davey, B. Funnell
semanticscholar +1 more source
We reconstituted Synechocystis glycogen synthesis in vitro from purified enzymes and showed that two GlgA isoenzymes produce glycogen with different architectures: GlgA1 yields denser, highly branched glycogen, whereas GlgA2 synthesizes longer, less‐branched chains.
Kenric Lee +3 more
wiley +1 more source
Active Stable Maintenance Functions in Low Copy-Number Plasmids of Gram-Positive Bacteria I. Partition Systems [PDF]
Low copy number plasmids cannot rely on the random segregation during bacterial cell division. To be stably maintained in the population they evolved two types of mechanisms (i) partition systems (PAR) that actively separate replicated plasmid molecules ...
Dmowski, Michal, Jagura-Burdzy, Grazyna
core

