Results 111 to 120 of about 833,514 (313)

Biophysical characterization and ion transport with cell‐based and proteoliposome reconstitution assays of invertebrate K+‐Cl− cotransporters

FEBS Open Bio, EarlyView.
The K+‐Cl− cotransporters (KCCs) facilitate the symport of ions across the plasma membrane. They participate in physiological processes including neuronal regulation. Here, we characterized KCCs from Drosophila and Hydra vulgaris. Comparative analyses of transporters provide insights into the mechanism of KCC ion transport, regulation, and evolution ...
Satoshi Fudo, Marina Verkhovskaya, Coralie Di Scala, Claudio Rivera, Tommi Kajander   +4 more
wiley   +1 more source

Detection of R Plasmids in Naturally Occurring Fish‐Pathogenic Bacteria, Edwardsiella tarda [PDF]

, 1977
Takashi Aoki, Toshihiko Arai, Syuzo EGUSA   +2 more
openalex   +1 more source

An efficient strategy for producing RNA‐free Nucleocapsid protein of SARS‐CoV‐2 for biochemical and structural investigations

FEBS Open Bio, EarlyView.
Cleavable N‐terminal Thioredoxin fusion enabled soluble expression and purification of otherwise insoluble SARS‐CoV‐2 Nucleocapsid (N) protein. A four‐step purification strategy yielded highly homogeneous, RNA‐free N protein. Binding assays showed high RNA affinity (Kd ~ 28 nm). The study will facilitate high‐resolution structural studies of N protein,
Shweta Singh, Gagan D. Gupta
wiley   +1 more source

THE POPULATION BIOLOGY OF BACTERIAL PLASMIDS: A PRIORI CONDITIONS FOR THE EXISTENCE OF CONJUGATIONALLY TRANSMITTED FACTORS [PDF]

, 1977
Frank M. Stewart, Bruce R. Levin
openalex   +1 more source

Studies on transformation of Escherichia coli with plasmids.

Journal of Molecular Biology, 1983
D. Hanahan, D. Hanahan
semanticscholar   +1 more source

Analysis of the regulation of undecaprenyl diphosphate dephosphorylation in Escherichia coli

FEBS Open Bio, EarlyView.
BacA, PgpB, and YbjG phosphatases are involved in undecaprenyl phosphate (C55P) synthesis in Escherichia coli. We analyzed the lipid contents and the gene expression in the gene‐disruption strains. Undecaprenyl diphosphate (C55PP) level increased in the bacA, ybjG double‐disruption strain, but C55P levels were similar in all strains.
Tomotaka Jitsukawa, Yasushi Shigeri, Shingo Fujisaki   +2 more
wiley   +1 more source

Editorial: Enterobacteriaceae Antimicrobial Agents and Resistance: Relationship With the Therapeutic Approach

Frontiers in Cellular and Infection Microbiology, 2021
Maria Teresa Mascellino, Silpak Biswas, Alessandra Oliva   +2 more
doaj   +1 more source

Method for the isolation of the replication region of a bacterial replicon: construction of a mini-F'kn plasmid [PDF]

, 1976
Michael Lovett, Donald R. Helinski
openalex   +1 more source

In Silico Detection and Typing of Plasmids using PlasmidFinder and Plasmid Multilocus Sequence Typing

Antimicrobial Agents and Chemotherapy, 2014
A. Carattoli, E. Zankari, A. García-Fernández, Mette Voldby Larsen, O. Lund, L. Villa, Frank Møller Aarestrup, H. Hasman   +7 more
semanticscholar   +1 more source

Co‐overexpression of the caloric restriction‐induced mitochondrial factors PGC‐1α and MIPEP upregulates Phospho1 expression in adipocytes

FEBS Open Bio, EarlyView.
Caloric restriction that extends lifespan induces the expression of PGC‐1α and MIPEP in white adipose tissue. In this study, co‐overexpression of Pgc‐1α and Mipep upregulated the gene expression of PHOSPHO1. These findings provide new insights into mitochondria‐related mechanisms underlying the effects of caloric restriction in adipocytes.
Mamiko Ishimatsu, Kanari Taki, Asuka Hayami, Komachi Kato, Yuka Nozaki, Yuhei Mizunoe, Takumi Narita, Ryoichi Mori, Yoshikazu Higami, Masaki Kobayashi   +9 more
wiley   +1 more source