Results 51 to 60 of about 1,104,645 (398)

Precise targeted integration by a chimaeric transposase zinc-finger fusion protein [PDF]

, 2010
Transposons of the Tc1/mariner family have been used to integrate foreign DNA stably into the genome of a large variety of different cell types and organisms.
Abdel-Meguid, Amann, Amy L. Bednarz, Beerli, Boyd, Bryan, Bushman, Bushman, Ciuffi, Cockrell, Collins, Daniel, Emmons, Feng, Goulaouic, Hacein-Bey-Abina, Isalan, Ivics, Ivics, Ivics, Ivics, Ivics, Kaneko, Kaneko, Katz, Lampe, Lampe, Lohe, Maeder, Maragathavally, Ni, Pabo, Pavletich, Plasterk, Richardson, Rimphanitchayakit, Sean D. Colloms, Simons, Sorrell, Suckow, Szabo, Tan, Tan, Thomas, Urasaki, Voigt, Wilson, Xiaofeng Feng, Yant, Zhu   +49 more
core   +3 more sources

Thermostable neutral metalloprotease from Geobacillus sp. EA1 does not share thermolysin's preference for substrates with leucine at the P1′ position

FEBS Letters, EarlyView.
Knowing how proteases recognise preferred substrates facilitates matching proteases to applications. The S1′ pocket of protease EA1 directs cleavage to the N‐terminal side of hydrophobic residues, particularly leucine. The S1′ pocket of thermolysin differs from EA's at only one position (leucine in place of phenylalanine), which decreases cleavage ...
Grant R. Broomfield, Torsten Kleffmann, David J. Saul, Sigurd M. Wilbanks   +3 more
wiley   +1 more source

Phenotypic and genetic analysis of Klebsiella pneumoniae strains isolated from community-acquired pneumonia patients in Rostov-on-Don in 2021–2023

Инфекция и иммунитет
Introduction. Here, we present a study of 33 Klebsiella pneumoniae strains isolated in Rostov-on-Don in 2021–2023 from patients with community-acquired pneumonia.
Violetta A. Rykova, O. N. Podladchikova, A. S. Anisimova, N. V. Aronova, A. S. Vodopyanov, S. Yu. Temyakova, E. N. Gudueva   +6 more
doaj   +1 more source

Toxin-Antitoxin Systems in Clinical Pathogens

Toxins, 2016
Toxin-antitoxin (TA) systems are prevalent in bacteria and archaea. Although not essential for normal cell growth, TA systems are implicated in multiple cellular functions associated with survival under stress conditions. Clinical strains of bacteria are
Laura Fernández-García, Lucia Blasco, Maria Lopez, German Bou, Rodolfo García-Contreras, Thomas Wood, María Tomas   +6 more
doaj   +1 more source

Molecular characterization of NDM-1-producing carbapenem-resistant E. cloacae complex from a tertiary hospital in Chongqing, China

Frontiers in Cellular and Infection Microbiology, 2022
BackgroundThe aim of this study was to clarify the molecular characterization of NDM-1-producing carbapenem-resistant Enterobacter cloacae complex (CREL) at a teaching hospital in Chongqing, China.MethodsAntimicrobial susceptibility and resistance genes ...
Kewang Hu, Kewang Hu, Jisheng Zhang, Jingbo Zou, Lingyi Zeng, Lingyi Zeng, Jie Li, Jianmin Wang, Wenzhang Long, Xiaoli Zhang   +9 more
doaj   +1 more source

C‐mannosylation promotes ADAMTS1 activation and secretion in human testicular germ cell tumor NEC8 cells

FEBS Letters, EarlyView.
C‐mannosylation is a unique form of protein glycosylation. In this study, we demonstrated that ADAMTS1 is C‐mannosylated at Trp562 and Trp565 in human testicular germ cell tumor NEC8 cells. We found that C‐mannosylation of ADAMTS1 is essential for its secretion, processing, enzymatic activity, and ability to promote vasculogenic mimicry. These findings
Takato Kobayashi, Takehiro Suzuki, Ryota Kawahara, Natsumi Harai, Naoshi Dohmae, Siro Simizu   +5 more
wiley   +1 more source

The antibiotic sensitivity patterns and plasmid DNA content of gram-negative anaerobic bacteria isolated in Palmerston North, New Zealand : a thesis presented in partial fulfilment of the requirements for the degree of Masters in Science at Massey University [PDF]

, 1987
One hundred and seven Gram-negative bacteria, including 65 Bacteroides species, 28 fusobacteria and 14 veillonellae were isolated from 17 oral infections treated in two dental surgeries in Palmerston North.
Mooney, Christopher Allan
core  

Sustained gene expression in the retina by improved episomal vectors [PDF]

, 2014
Gene and cellular therapies are nowadays part of therapeutic strategies for the treatment of diverse pathologies. The drawbacks associated with gene therapy-low levels of transgene expression, vector loss during mitosis, and gene silencing-need to be ...
Ana V. Oliveira, Conley S.M., Davis A.A., Gabriela A. Silva, Reinisalo M., Rudolf Haase, Sofia M. Calado, Susana Machado   +7 more
core   +1 more source

The multidrug and toxin extrusion (MATE) transporter DTX51 antagonizes non‐cell‐autonomous HLS1–AMP1 signaling in a region‐specific manner

FEBS Letters, EarlyView.
The Arabidopsis mutants hls1 hlh1 and amp1 lamp1 exhibit pleiotropic developmental phenotypes. Although the functions of the causative genes remain unclear, they act in the same genetic pathway and are thought to generate non‐cell‐autonomous signals.
Takashi Nobusawa, Makoto Kusaba
wiley   +1 more source

Global phylogenetic analysis of Escherichia coli and plasmids carrying the mcr-1 gene indicates bacterial diversity but plasmid restriction

Scientific Reports, 2017
To understand the dynamics behind the worldwide spread of the mcr-1 gene, we determined the population structure of Escherichia coli and of mobile genetic elements (MGEs) carrying the mcr-1 gene. After a systematic review of the literature we included 65
S. Matamoros, Jarne M. van Hattem, M. Arcilla, Niels Willemse, D. Melles, J. Penders, T. N. Vinh, Ngo Thi Hoa, M. Bootsma, P. V. van Genderen, A. Goorhuis, M. Grobusch, Nicky Molhoek, A. O. Oude Lashof, E. Stobberingh, H. Verbrugh, M. D. de Jong, C. Schultsz   +17 more
semanticscholar   +1 more source