Results 51 to 60 of about 833,514 (313)
FEBS Letters, EarlyView.Dimeric pyruvate formate‐lyase cleaves pyruvate using a radical‐based mechanism. G734 serves as a radical storage location, and the radical is transferred to the catalytic C419 residue. Mutation of the C418‐C419 pair causes loss of enzyme activity, but does not impede radical introduction onto G734. Therefore, cis‐ but not trans‐radical transfer occurs
Michelle Kammel +2 more
wiley +1 more source
Disruption of SETD3‐mediated histidine‐73 methylation by the BWCFF‐associated β‐actin G74S mutation
FEBS Letters, EarlyView.The β‐actin G74S mutation causes altered interaction of actin with SETD3, reducing histidine‐73 methylation efficiency and forming two distinct actin variants. The variable ratio of these variants across cell types and developmental stages contributes to tissue‐specific phenotypical changes. This imbalance may impair actin dynamics and mechanosensitive
Anja Marquardt +8 more
wiley +1 more source
Toxin-Antitoxin Systems in Clinical Pathogens
Toxins, 2016 Toxin-antitoxin (TA) systems are prevalent in bacteria and archaea. Although not essential for normal cell growth, TA systems are implicated in multiple cellular functions associated with survival under stress conditions. Clinical strains of bacteria are
Laura Fernández-García +6 more
doaj +1 more source
A DNA transfection system for generation of influenza A virus from eight plasmids.
Proceedings of the National Academy of Sciences of the United States of America, 2000 We have developed an eight-plasmid DNA transfection system for the rescue of infectious influenza A virus from cloned cDNA. In this plasmid-based expression system, viral cDNA is inserted between the RNA polymerase I (pol I) promoter and terminator ...
E. Hoffmann +4 more
semanticscholar +1 more source
FEBS Letters, EarlyView.This study reveals how prime editing guide RNA (pegRNA) secondary structure and reverse transcriptase template length affect prime editing efficiency in correcting the phospholamban R14del cardiomyopathy‐associated mutation. Insights support the design of structurally optimized enhanced pegRNAs for precise gene therapy.
Bing Yao +7 more
wiley +1 more source
On the (Im)possibility to Reconstruct Plasmids from Whole Genome Short-Read Sequencing Data
bioRxiv, 2017 Plasmids are autonomous extra-chromosomal elements in bacterial cells that can carry genes that are important for bacterial survival. To benchmark algorithms for automated plasmid sequence reconstruction from short read sequencing data, we selected 42 ...
S. Arredondo-Alonso +3 more
semanticscholar +1 more source
TRAF2 binds to TIFA via a novel motif and contributes to its autophagic degradation
FEBS Letters, EarlyView.TRAF family members couple receptor signalling complexes to downstream outputs, but how they interact with these complexes is not always clear. Here, we show that during ADP‐heptose signalling, TRAF2 binding to TIFA requires two short sequence motifs in the C‐terminal tail of TIFA, which are distinct from the TRAF6 binding motif.
Tom Snelling +4 more
wiley +1 more source
Pathogens, 2020 We aimed to investigate the occurrence of acquired AmpC β-lactamases (qAmpC), and characterize qAmpC-producing Enterobacteriaceae from different non-clinical environments in Portugal.
Teresa Gonçalves Ribeiro +3 more
doaj +1 more source
Plants, 2022 Xylella fastidiosa (Xf) is a phytopathogenic bacterium with a repertoire of self-replicating genetic elements, including plasmids, pathogenicity islands, and prophages. These elements provide potential avenues for horizontal gene transfer both within and
María Pilar Velasco-Amo +5 more
doaj +1 more source
Fitness Advantage of mcr-1–Bearing IncI2 and IncX4 Plasmids in Vitro
Frontiers in Microbiology, 2018 The objective of this study was to assess the impact of diverse plasmids bearing colistin resistance gene mcr-1 on host fitness. Forty-seven commensal E.
Renjie Wu +8 more
semanticscholar +1 more source