Results 71 to 80 of about 1,080,559 (427)
In 2014–2015, the main CTX-M-15- and OXA-48-producing clone in our region was ST15. Recently, K. pneumoniae ST15 isolates co-producing VIM-1 and CTX-M-15 were detected in several hospitals.
Patricia Pérez-Palacios+6 more
doaj +1 more source
A DNA transfection system for generation of influenza A virus from eight plasmids.
We have developed an eight-plasmid DNA transfection system for the rescue of infectious influenza A virus from cloned cDNA. In this plasmid-based expression system, viral cDNA is inserted between the RNA polymerase I (pol I) promoter and terminator ...
E. Hoffmann+4 more
semanticscholar +1 more source
Heteroduplex analysis of tra delta f' plasmids and the mechanism of their formation [PDF]
Four tra delta FargG+ plasmids, derived from matings between Hfr AB312 and a recA recipient, have been shown to have deletions of at least 50% of the F genome, including the region in which the tra genes map.
Clark, Alvin J.+2 more
core +1 more source
Expression and regulatory effects on cancer cell behavior of NELL1 and NELL2 in human renal cell carcinoma [PDF]
We thank Professors Michael Rehli, Yoshiaki Ito, and Kristian Helin for gifting plasmids, Dr. Alasdair MacKenzie (University of Aberdeen) for helpful discussion, and Mr. Takashi Mizukami, Ms. Ryoko Tokuda, and Ms.
Mizokami, Atsushi+6 more
core +1 more source
We present the cellular transcription‐coupled Flp‐nick system allowing the introduction of a Top1‐mimicking cleavage complex (Flpcc) at a Flp recognition target site within a controllable LacZ gene. LacZ transcription leads to the collision of RNA polymerase II (RNAPII) with Flpcc, and this causes RNAPII stalling, ubiquitination, and degradation.
Petra Herring+6 more
wiley +1 more source
Antimicrobial resistance is a global public health concern, and resistance genes in Salmonella, especially those located on mobile genetic elements, are part of the problem.
Abdurrahman Hassan Jibril+4 more
doaj +1 more source
On the (Im)possibility to Reconstruct Plasmids from Whole Genome Short-Read Sequencing Data
Plasmids are autonomous extra-chromosomal elements in bacterial cells that can carry genes that are important for bacterial survival. To benchmark algorithms for automated plasmid sequence reconstruction from short read sequencing data, we selected 42 ...
S. Arredondo-Alonso+3 more
semanticscholar +1 more source
Finished Genome Sequences of Xanthomonas fragariae, the Cause of Bacterial Angular Leaf Spot of Strawberry. [PDF]
Xanthomonas fragariae is a foliar pathogen of strawberry that is of significant concern to nursery production of strawberry transplants and field production of strawberry fruit.
Henry, Peter M, Leveau, Johan HJ
core +2 more sources
ABSTRACTPlasmids are currently an indispensable molecular tool in life science research and a central asset for the modern biotechnology industry, supporting its mission to produce pharmaceutical proteins, antibodies, vaccines, industrial enzymes, and molecular diagnostics, to name a few key products.
Duarte Miguel F, Prazeres+1 more
openaire +2 more sources
Ion channel function of polycystin‐2/polycystin‐1 heteromer revealed by structure‐guided mutagenesis
Mutations in polycystin‐1 (PC1) or polycystin‐2 (PC2) cause autosomal‐dominant polycystic kidney disease (ADPKD). We generated a novel gain‐of‐function PC2/PC1 heteromeric ion channel by mutating pore‐blocking residues. Moreover, we demonstrated that PC2 will preferentially assemble with PC1 to form heteromeric complexes when PC1 is co‐expressed ...
Tobias Staudner+7 more
wiley +1 more source