Results 71 to 80 of about 1,080,559 (427)

Interhospital Spread of blaVIM-1- and blaCTX-M-15-Producing K. pneumoniae ST15 on an IncR Plasmid in Southern Spain

open access: yesAntibiotics, 2023
In 2014–2015, the main CTX-M-15- and OXA-48-producing clone in our region was ST15. Recently, K. pneumoniae ST15 isolates co-producing VIM-1 and CTX-M-15 were detected in several hospitals.
Patricia Pérez-Palacios   +6 more
doaj   +1 more source

A DNA transfection system for generation of influenza A virus from eight plasmids.

open access: yesProceedings of the National Academy of Sciences of the United States of America, 2000
We have developed an eight-plasmid DNA transfection system for the rescue of infectious influenza A virus from cloned cDNA. In this plasmid-based expression system, viral cDNA is inserted between the RNA polymerase I (pol I) promoter and terminator ...
E. Hoffmann   +4 more
semanticscholar   +1 more source

Heteroduplex analysis of tra delta f' plasmids and the mechanism of their formation [PDF]

open access: yes, 1977
Four tra delta FargG+ plasmids, derived from matings between Hfr AB312 and a recA recipient, have been shown to have deletions of at least 50% of the F genome, including the region in which the tra genes map.
Clark, Alvin J.   +2 more
core   +1 more source

Expression and regulatory effects on cancer cell behavior of NELL1 and NELL2 in human renal cell carcinoma [PDF]

open access: yes, 2015
We thank Professors Michael Rehli, Yoshiaki Ito, and Kristian Helin for gifting plasmids, Dr. Alasdair MacKenzie (University of Aberdeen) for helpful discussion, and Mr. Takashi Mizukami, Ms. Ryoko Tokuda, and Ms.
Mizokami, Atsushi   +6 more
core   +1 more source

A cellular system to study responses to a collision between the transcription complex and a protein‐bound nick in the DNA template

open access: yesFEBS Letters, EarlyView.
We present the cellular transcription‐coupled Flp‐nick system allowing the introduction of a Top1‐mimicking cleavage complex (Flpcc) at a Flp recognition target site within a controllable LacZ gene. LacZ transcription leads to the collision of RNA polymerase II (RNAPII) with Flpcc, and this causes RNAPII stalling, ubiquitination, and degradation.
Petra Herring   +6 more
wiley   +1 more source

Genomic Analysis of Antimicrobial Resistance and Resistance Plasmids in Salmonella Serovars from Poultry in Nigeria

open access: yesAntibiotics, 2021
Antimicrobial resistance is a global public health concern, and resistance genes in Salmonella, especially those located on mobile genetic elements, are part of the problem.
Abdurrahman Hassan Jibril   +4 more
doaj   +1 more source

On the (Im)possibility to Reconstruct Plasmids from Whole Genome Short-Read Sequencing Data

open access: yesbioRxiv, 2017
Plasmids are autonomous extra-chromosomal elements in bacterial cells that can carry genes that are important for bacterial survival. To benchmark algorithms for automated plasmid sequence reconstruction from short read sequencing data, we selected 42 ...
S. Arredondo-Alonso   +3 more
semanticscholar   +1 more source

Finished Genome Sequences of Xanthomonas fragariae, the Cause of Bacterial Angular Leaf Spot of Strawberry. [PDF]

open access: yes, 2016
Xanthomonas fragariae is a foliar pathogen of strawberry that is of significant concern to nursery production of strawberry transplants and field production of strawberry fruit.
Henry, Peter M, Leveau, Johan HJ
core   +2 more sources

Plasmid Biopharmaceuticals

open access: yesMicrobiology Spectrum, 2014
ABSTRACTPlasmids are currently an indispensable molecular tool in life science research and a central asset for the modern biotechnology industry, supporting its mission to produce pharmaceutical proteins, antibodies, vaccines, industrial enzymes, and molecular diagnostics, to name a few key products.
Duarte Miguel F, Prazeres   +1 more
openaire   +2 more sources

Ion channel function of polycystin‐2/polycystin‐1 heteromer revealed by structure‐guided mutagenesis

open access: yesFEBS Letters, EarlyView.
Mutations in polycystin‐1 (PC1) or polycystin‐2 (PC2) cause autosomal‐dominant polycystic kidney disease (ADPKD). We generated a novel gain‐of‐function PC2/PC1 heteromeric ion channel by mutating pore‐blocking residues. Moreover, we demonstrated that PC2 will preferentially assemble with PC1 to form heteromeric complexes when PC1 is co‐expressed ...
Tobias Staudner   +7 more
wiley   +1 more source

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