Results 91 to 100 of about 48,469 (263)

Gene Editing of Nicotiana benthamiana Architecture for Space‐Efficient Production of Recombinant Proteins in Closed Environments

open access: yesPlant Biotechnology Journal, EarlyView.
ABSTRACT Indoor vertical farming (VF) offers practical advantages for the cultivation of plant protein bio‐factories including plant uniformity, product consistency, water/nutrient recycling and production cycles on a year‐round basis. Much progress has been achieved toward the development of innovative systems for artificial lighting, automated ...
Béatrice Giroux   +5 more
wiley   +1 more source

Design and Immunogenicity of a Nanoparticle Combination Vaccine for Salmonella in Poultry

open access: yesPlant Biotechnology Journal, EarlyView.
ABSTRACT Salmonella enterica serovars (Salmonella) are common causes of bacterial gastroenteritis (salmonellosis) often associated with the consumption of poultry products. The control of Salmonella in broiler chickens is difficult because colonized birds may carry this pathogen undetected into the slaughterhouse and cause meat contamination.
Shabnam Shamriz   +6 more
wiley   +1 more source

RNA‐Guided Engineering of the Chloroplast Genome Enabled by Plastid‐Expressed Guide RNAs

open access: yesPlant Biotechnology Journal, EarlyView.
ABSTRACT Our goal is to develop RNA‐guided engineering of the chloroplast genome using the CRISPR/Cas9 system. We designed chloroplast minigenes to obtain properly sized single guide RNAs (sgRNAs) in tobacco chloroplasts. The sgRNA 5′ end is defined by transcription from an rRNA operon promoter, and its 3′ end by processing a downstream tRNA (trnG) or ...
Malihe Mirzaee   +3 more
wiley   +1 more source

Evolution‐Inspired Engineering of Diterpene Biosynthesis via Chloroplast Genome Modification

open access: yesPlant Biotechnology Journal, EarlyView.
ABSTRACT Terpenes constitute the largest and most structurally diverse class of plant secondary metabolites, with critical roles in plant‐environment interactions and broad industrial applications. Although nuclear genome engineering of terpene pathways has been extensively explored, chloroplast genome engineering remains largely undeveloped, with most
Alessandro Occhialini   +6 more
wiley   +1 more source

Bacteriophage P22 Virus‐Like Particles as Nanoscale Protein Scaffolds for Plant Synthetic Biology

open access: yesPlant Biotechnology Journal, EarlyView.
ABSTRACT Advancing the utility of plant synthetic biology requires the continued development of protein engineering tools. Self‐assembling protein compartments, such as virus‐like particles (VLPs), provide versatile scaffolds for synthetic biology. However, few plant‐expressed VLPs have demonstrated broad amenability to protein engineering, restricting
Maxim D. Harding   +5 more
wiley   +1 more source

The OsWRKY15‐OsSUT3 Regulatory Module Enhances Rice Pollen Fertility by Mediating Sucrose Allocation to Anthers

open access: yesPlant Biotechnology Journal, EarlyView.
ABSTRACT Sucrose transport and starch accumulation are crucial carbohydrate metabolic processes in rice, playing essential roles during late pollen development to ensure pollen maturation, fertility and high grain production. However, the molecular mechanisms regulating sucrose transport and starch accumulation during rice pollen development remain ...
Qiuping Li   +10 more
wiley   +1 more source

Unexpected Suppression of Tomato Fruit Cuticle Formation by Overexpression of a Key Cutin Biosynthetic Enzyme

open access: yesPlant Biotechnology Journal, EarlyView.
ABSTRACT Fruit cuticles regulate the diffusion of water, gases and solutes, functioning as essential protective interfaces against environmental and biotic stresses. Engineering cuticle structure and mechanics therefore represents a promising strategy for improving fruit quality and resilience.
Gulab Chand Arya   +5 more
wiley   +1 more source

Metabolic Rewiring of the Shikimate Pathway Enables Rose‐Scented Rice

open access: yesPlant Biotechnology Journal, EarlyView.
ABSTRACT Rice fragrance is a key determinant of grain quality, yet the aroma profile of cultivated rice is highly uniform and largely dominated by the popcorn‐like compound 2‐acetyl‐1‐pyrroline. Here we expanded the aromatic repertoire of rice by engineering a rose‐like fragrance through reconstruction of the phenylalanine‐derived 2‐phenylethanol (2‐PE)
Xiaoqing Cui   +11 more
wiley   +1 more source

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