Results 121 to 130 of about 173,543 (315)

Ultrasound‐Actuated Gene Editing in Human Kidney Organoids

Advanced Science, EarlyView.
Peptide‐stabilized nanoemulsions enable spatially controlled, ultrasound‐triggered delivery of gene‐editing proteins deep within human kidney organoid tissues. Focused acoustic pulses drive droplet vaporization, propelling cargo into target renal cells while preserving native tissue microarchitecture. This non‐viral platform improves both the depth and
Michael A. Miller, Nicole Vo, Utkarsh, Ivan Sokirniy, Justin Pritchard, Benjamin S. Freedman, Scott H. Medina   +6 more
wiley   +1 more source

Deriving bovine embryonic stem-like cells in defined conditions

, 2011
The first embryonic stem ES cell line was isolated from mouse in 1981 (Evans and Kaufman 1981; Martin1981). However, ESCs are only available in rodent species.
Muhammad Alahdal, Hadil, Hadil Muhammad Alahdal   +1 more
core  

Endoplasmic Reticulum Geometry Dictates Neuronal Bursting via Calcium Store Refill Rates and Exposes Selective Neuronal Vulnerability

Advanced Science, EarlyView.
The ER's continuous tubular network is maintained by ER‐shaping proteins whose mutation or dysregulation contributes to neurodegenerative diseases. Here, we show that ER morphology sets the speed of Ca2+ store replenishment between firing events. Disrupting ER continuity slows intra‐ER Ca2+ redistribution from extracellular refill (SOCE) sites, driving
Valentina Davi, Pierre Parutto, Yuyi Zhang, Tasuku Konno, Cecile Crapart, Raquel Pereira, John P. Franklin, Mosab Ali Awadelkareem, Daniel C Maddison, Michael J. Devine, Edgar R. Gomes, Joseph Chambers, Elena Koslover, Edward Avezov   +13 more
wiley   +1 more source

S251: DISRUPTION OF SUCCINATE RECEPTOR SIGNALING PROMOTES MYELOPROLIFERATION AND ACUTE MYELOID LEUKAEMIA

HemaSphere, 2022
V. Cuminetti, J. Konieczny, A. Bernal, A. Villatoro, H. Taman, M. Ristic, N. Villaplana-Lopera, R. H. Paulssen, G. Giovinazzo, A. Vik, P. Garcia, L. Arranz   +11 more
doaj   +1 more source

DNA Methylation Is Involved in the Expression of miR-142-3p in Fibroblasts and Induced Pluripotent Stem Cells

Stem Cells International, 2014
MicroRNAs are differentially expressed in cells and regulate multiple biological processes. We have been analyzing comprehensive expression patterns of microRNA in human and mouse embryonic stem and induced pluripotent stem cells. We determined microRNAs
Siti Razila Abdul Razak, Yukihiro Baba, Hiromitsu Nakauchi, Makoto Otsu, Sumiko Watanabe   +4 more
doaj   +1 more source

Cell-free Embryonic Stem Cell Extract-mediated Derivation of Multi-potent Stem Cells from NIH3T3 Fibroblasts for Functional and Anatomical Ischemic Tissue Repair

, 2007
The oocyte-independent generation of multipotent stem cells is one of the goals in regenerative medicine. We report that upon exposure to mouse ES cell (ESC) extracts, reversibly permeabilized NIH3T3 cells undergo de-differentiation followed by stimulus ...
Erin Lambers, Johnson Rajasingh, Hiromichi Hamada, Deepali Ahluwalia, Tina Thorne, Ilona Goukassian, Kenneth M. Rosen, Yan Zhu, Douglas W. Losordo, Evelyn Bord, Prasanna Krishnamurthy, Gangjian Qin, Karen Schlauch, Raj Kishore   +13 more
core  

A Circuit of Mechanically Regulated Transcription Factors Balances Regenerative and Fibrotic Memory of Mesenchymal Stromal Cells

Advanced Science, EarlyView.
Producing MSCs on rigid culture substrates induces a scar‐making phenotype, jeapordizing therapeutic success. ‘Tissue‐soft’ surfaces prevent MSC fibrogenesis and preserve regenerative traits. An epigenetic network, driven by HOXA11 and SALL1, maintains ‘soft memory’ by keeping chromatin open in relaxed MSCs, promoting anti‐fibrotic programs.
Fereshteh Sadat Younesi, Andrew E. Miller, Li Diao, Xinying Guo, Natalie Andonian, Elham Karimizadeh, Thomas H. Barker, Boris Hinz   +7 more
wiley   +1 more source

Stepwise Differentiation of Retinal Ganglion Cells from Human Pluripotent Stem Cells Enables Analysis of Glaucomatous Neurodegeneration

, 2016
Human pluripotent stem cells (hPSCs), including both embryonic and induced pluripotent stem cells, possess the unique ability to readily differentiate into any cell type of the body, including cells of the retina.
Sridhar, Akshayalakshmi, Cummins, Theodore R., Sarfarazi, Mansoor, Meyer, Jason S., Ohlemacher, Sarah K., Hochstetler, Alexandra E., Xiao, Yucheng   +6 more
core   +1 more source

Developmentally Inspired Bioprinting of Nascent Multicellular Human Heart Tissue Through in Situ Differentiation and Morphogenesis of iPSCs

Advanced Science, EarlyView.
A developmentally inspired bioprinting approach enables the fabrication of pluripotent tissues that undergo shape‐morphing and in situ cardiac lineage specification. This method employs embedded bioprinting to deposit iPSCs within soft granular hydrogels to create pluripotent tissue constructs that undergo cell‐mediated shape morphogenesis.
Ankita Pramanick, Juhi Chakraborty, Orlaith Kennedy, Hey Wei Wong, Sogol Kianersi, Daniel Kelly, Vasileios Sergis, Abhay Pandit, Andrew C. Daly   +8 more
wiley   +1 more source

Efficient Scaling up EV‐AAVs Production via Cellular Nanoporation for Familial Hypercholesterolaemia Therapy

Journal of Extracellular Vesicles
Adeno‐associated virus (AAV)–mediated gene therapies face critical clinical limitations, including immune‐mediated neutralization by pre‐existing antibodies and dose‐dependent hepatotoxicity.
Yuting Yan, Yi You, Shuhong Ma, Hui Yi, Guangduo Chen, Jie Ni, Changyan Chen, Wenyu Ke, Lingying Li, Rui Bai, Yuqing Ran, Wenjing Lu, Min Zhu, Yongshuai Zhang, Jing Dai, Man Qi, Feng Lan, Andrew S. Lee, Ran Zhang, Xujie Liu, Zhaoyang Chen   +20 more
doaj   +1 more source