Results 171 to 180 of about 2,826 (206)
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Analytical Biochemistry, 2004
ARChip Epoxy and ARChip UV are presented as novel chip platforms for oligonucleotide immobilization. ARChip Epoxy is made of reactive epoxy resin available commercially. ARChip UV consists of photoactivatable poly(styrene-co-4-vinylbenzylthiocyanate).
Claudia, Preininger +4 more
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ARChip Epoxy and ARChip UV are presented as novel chip platforms for oligonucleotide immobilization. ARChip Epoxy is made of reactive epoxy resin available commercially. ARChip UV consists of photoactivatable poly(styrene-co-4-vinylbenzylthiocyanate).
Claudia, Preininger +4 more
openaire +2 more sources
Current Microbiology, 2017
Nitrite-dependent anaerobic methane oxidation (n-damo) is catalyzed by the NC10 phylum bacterium "Candidatus Methylomirabilis oxyfera" (M. oxyfera). Generally, the pmoA gene is applied as a functional marker to test and identify NC10-like bacteria. However, it is difficult to detect the NC10 bacteria from sediments of freshwater lake (Dongchang Lake ...
Shenghui, Wang +4 more
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Nitrite-dependent anaerobic methane oxidation (n-damo) is catalyzed by the NC10 phylum bacterium "Candidatus Methylomirabilis oxyfera" (M. oxyfera). Generally, the pmoA gene is applied as a functional marker to test and identify NC10-like bacteria. However, it is difficult to detect the NC10 bacteria from sediments of freshwater lake (Dongchang Lake ...
Shenghui, Wang +4 more
openaire +2 more sources
The evolution of the AMOA, PMOA and BMOA genes measured by ka/ks ratios.
Proceedings of the West Virginia Academy of Science, 2016The copper-containing membrane-bound monooxygenase enzyme family consists of enzymes facilitating the oxidation of methane (catalyzed by particulate methane monooxygenase, pMMO) and ammonia (catalyzed by ammonia monooxygenase, AMO), in both aerobic and anaerobic methanotrophs and nitrifiers, respectively, and the oxidation of C2-10 alkanes (catalyzed ...
Evan Lau, Daniel Lukich, Tuan Le
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Journal of Biotechnology, 2018
A broad methanotrophic community consisting of 16 different operational taxonomic units (OTUs) was detected by particulate methane monooxygenase A (pmoA) gene analyses of reactor sludge samples obtained from an industrial biogas plant. Using a cloning-sequencing approach, 75% of the OTUs were affiliated to the group of type I methanotrophs (γ ...
Tobias, May +5 more
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A broad methanotrophic community consisting of 16 different operational taxonomic units (OTUs) was detected by particulate methane monooxygenase A (pmoA) gene analyses of reactor sludge samples obtained from an industrial biogas plant. Using a cloning-sequencing approach, 75% of the OTUs were affiliated to the group of type I methanotrophs (γ ...
Tobias, May +5 more
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Applied Microbiology and Biotechnology, 2013
A new pmoA gene-based PCR primer set was designed for detection of nitrite-dependent anaerobic oxidation of methane (n-damo) bacteria from four different ecosystems, namely rice paddy soil, freshwater reservoir, reed bed, and sludge from wastewater treatment plant.
Gu, J, Han, P
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A new pmoA gene-based PCR primer set was designed for detection of nitrite-dependent anaerobic oxidation of methane (n-damo) bacteria from four different ecosystems, namely rice paddy soil, freshwater reservoir, reed bed, and sludge from wastewater treatment plant.
Gu, J, Han, P
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Applied Soil Ecology, 2017
Abstract Methanotrophic bacteria in soil serve as the only known biological sink of atmospheric methane, and impact net methane emission from soils. Most members of methanotrophs use particulate methane monooxygenase enzyme, encoded by the pmoA gene, to oxidize methane.
Aditi Sengupta, Warren A. Dick
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Abstract Methanotrophic bacteria in soil serve as the only known biological sink of atmospheric methane, and impact net methane emission from soils. Most members of methanotrophs use particulate methane monooxygenase enzyme, encoded by the pmoA gene, to oxidize methane.
Aditi Sengupta, Warren A. Dick
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Biochemical Engineering Journal, 2019
Abstract A rapid recovery of methanotrophic activity from process fluctuations such as surges in CH4 inlet load is of utmost importance in bioreactors to guarantee a robust abatement of this potent greenhouse gas. However, the response of methanotrophs in CH4 abatement engineered ecosystems facing recurrent operational upsets has not been ...
Elisa Rodríguez +6 more
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Abstract A rapid recovery of methanotrophic activity from process fluctuations such as surges in CH4 inlet load is of utmost importance in bioreactors to guarantee a robust abatement of this potent greenhouse gas. However, the response of methanotrophs in CH4 abatement engineered ecosystems facing recurrent operational upsets has not been ...
Elisa Rodríguez +6 more
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Applied Microbiology and Biotechnology, 2014
In the present work, both 16S rRNA and pmoA gene-based PCR primers were employed successfully to study the diversity and distribution of n-damo bacteria in the surface and lower layer sediments at the coastal Mai Po wetland. The occurrence of n-damo bacteria in both the surface and subsurface sediments with high diversity was confirmed in this study ...
ZHOU, Z, Gu, J, CHEN, J
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In the present work, both 16S rRNA and pmoA gene-based PCR primers were employed successfully to study the diversity and distribution of n-damo bacteria in the surface and lower layer sediments at the coastal Mai Po wetland. The occurrence of n-damo bacteria in both the surface and subsurface sediments with high diversity was confirmed in this study ...
ZHOU, Z, Gu, J, CHEN, J
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Limnology, 2010
Spatiotemporal variations in microbial gene abundances were investigated to identify potential zones of methanotroph and methanogen biomass in a peat bog in Sarobetsu-genya wetland. The abundances of the bacterial and archaeal 16S rRNA genes, pmoA, and mcrA were 107–109, 107–108, 104–106, and 104–107 copies g−1 dry peat, respectively.
Masaru Akiyama +5 more
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Spatiotemporal variations in microbial gene abundances were investigated to identify potential zones of methanotroph and methanogen biomass in a peat bog in Sarobetsu-genya wetland. The abundances of the bacterial and archaeal 16S rRNA genes, pmoA, and mcrA were 107–109, 107–108, 104–106, and 104–107 copies g−1 dry peat, respectively.
Masaru Akiyama +5 more
openaire +1 more source
Applied Microbiology and Biotechnology, 2014
Nitrite-dependent anaerobic methane oxidation (n-damo) process is unique in linking the microbial carbon and nitrogen cycles, but the presence of n-damo bacteria in marine ecosystem and the associated environmental factors are still poorly understood. In the present study, detection of n-damo bacteria using 16S rRNA and pmoA gene-based PCR primers was ...
CHEN, J, Gu, J, ZHOU, Z
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Nitrite-dependent anaerobic methane oxidation (n-damo) process is unique in linking the microbial carbon and nitrogen cycles, but the presence of n-damo bacteria in marine ecosystem and the associated environmental factors are still poorly understood. In the present study, detection of n-damo bacteria using 16S rRNA and pmoA gene-based PCR primers was ...
CHEN, J, Gu, J, ZHOU, Z
openaire +4 more sources