Results 91 to 100 of about 2,353 (181)

Strategy to develop and validate digital droplet PCR methods for global antimicrobial resistance wastewater surveillance

Water Environment Research, Volume 96, Issue 11, November 2024.
Workflow for the development and validation of novel ddPCR methods for a harmonized and global AMR monitoring in wastewater. Abstract According to World Health Organization (WHO), antimicrobial resistance (AMR) is currently one of the world's top 10 health threats, causing infections to become difficult or impossible to treat, increasing the risk of ...
Andrea Gobbo, Marie‐Alice Fraiture, Laura Van Poelvoorde, Sigrid C. J. De Keersmaecker, Cristina Garcia‐Graells, Koenraad Van Hoorde, Bavo Verhaegen, Astrid Huwaert, Hadrien Maloux, Veronik Hutse, Pieter‐Jan Ceyssens, Nancy Roosens   +11 more
wiley   +1 more source

New insights into the mechanism of action of pyrazinamide, implications for susceptibility testing, and future regimens*

International Journal of Mycobacteriology, 2016
Pyrazinamide (PZA) is included in the 2016 World Health Organization multidrug-resistant tuberculosis treatment guidelines and is a key component of most ongoing clinical trials investigating novel antibiotic combinations.
Richard M Anthony, Alice den Hertog, Mikael Mansjö, Jim Werngren   +3 more
doaj   +1 more source

Insight into novel clinical mutants of RpsA-S324F, E325K, and G341R of Mycobacterium tuberculosis associated with pyrazinamide resistance

Computational and Structural Biotechnology Journal, 2018
Pyrazinamide (PZA) is an important component of first-line anti-tuberculosis drugs which is converted into active form, pyrazinoic acid (POA), by Mycobacterium tuberculosis (MTB) pncA gene encoded, pyrazinamidase (PZase).
Muhammad Tahir Khan, Ashfaq Ur Rehaman, Muhammad Junaid, Shaukat Iqbal Malik, Dong-Qing Wei   +4 more
doaj   +1 more source

The Study of PncA Gene Using PCR-RFLP and Allele-Specific PCR Methods in Distinguishing Mycobacterium Bovis from Mycobacterium Tuberculosis

مجله دانشکده پزشکی اصفهان, 2011
Background: Bovine tuberculosis is a chronic bacterial disease of cattle that occasionally affects human. A unique mutation at position 169 (C→G) in Mycobacterium bovis differentiates it from Mycobacterium tuberculosis. For the first time, PCR-RFLP method was used in this study to separate the two organisms.
Mohammad Torkaman, Fatemeh Maryam Sheikholslami, Parissa Farnia, Mohammad Hassan Shahhosseiny, Mohadeseh Mozafari, Mehdi Shams, Mohammad Reza Masjedi, Ali Akbar Velayati   +7 more
openalex   +2 more sources

[Investigation of pncA, rpsA and panD Gene Mutations Associated with Resistance in Pyrazinamide-Resistant Mycobacterium tuberculosis Isolates and Spoligotyping].

Mikrobiyoloji bulteni, 2022
Pyrazinamide (PZA) is one of the first-line anti-tuberculous drugs used in the treatment of tuberculosis (TB). Considering the ability of PZA to shorten the treatment period from 9-12 months to six months by eliminating persistent bacilli, it appears to be an important cornerstone of TB therapy.
Didem, Özgür, Seda, Tezcan Ülger, Mediha Begüm, Kayar, Can, Biçmen, Ahmet, Aslantürk, Mahmut, Ülger, Gönül, Aslan   +6 more
openaire   +1 more source

Estimation of pyrazinamidase activity using a cell-free In vitro synthesis of pnca and its association with pyrazinamide susceptibility in Mycobacterium tuberculosis

International Journal of Mycobacteriology, 2018
Background: The main mechanism of resistance to PZA in Mycobacterium tuberculosis relies on mutations on its pyrazinamidase/nicotinamidase. Recently, a rapid colorimetric test relying on the PCR-based in vitro-synthesized-PZase assay has been reported ...
Daniel Rueda, Christine Bernard, Lucas Gandy, Estelle Capton, Rachid Boudjelloul, Florence Brossier, Nicolas Veziris, Mirko Zimic, Wladimir Sougakoff   +8 more
doaj   +1 more source

Resistance To First-Line Antituberculosis Drugs And Prevalence Of pncA Mutations In Clinical Isolates Of Mycobacterium tuberculosis From Zunyi, Guizhou Province Of China

Infection and Drug Resistance, 2019
Zhimin Cao,1 Yuanbo Lan,1 Ling Chen,1 Min Xiang,1 Zhiyuan Peng,1 Jianyong Zhang,1 Hong Zhang1,2 1Tuberculosis Division, Department of Respiratory and Critical Care Medicine, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou 563003, People ...
Cao Z, Lan Y, Chen L, Xiang M, Peng Z, Zhang J, Zhang H   +6 more
doaj  

Molecular analysis of pyrazinamide resistance in Mycobacterium tuberculosis in Vietnam highlights the high rate of pyrazinamide resistance-associated mutations in clinical isolatesPncA mutations and PZA resistance in M. tuberculosis in Vietnam

Emerging Microbes and Infections, 2017
Pyrazinamide (PZA) is a key antibiotic in current anti-tuberculosis regimens. Although the WHO has stressed the urgent need to obtain data on PZA resistance, in high tuberculosis burden countries, little is known about the level of PZA resistance, the ...
Nguyen Quang Huy, Contamin Lucie, Tran Thi Thanh Hoa, Nguyen Van Hung, Nguyen Thi Ngoc Lan, Nguyen Thai Son, Nguyen Viet Nhung, Dang Duc Anh, Bañuls Anne-Laure, Nguyen Thi Van Anh   +9 more
doaj   +1 more source

Multidrug-resistant Myobacterium tuberculosis caused by the Beijing genotype and a specific T1 genotype clone (SIT No. 266) is widely transmitted in Minsk

International Journal of Mycobacteriology, 2013
Setting: This study was performed in the city of Minsk in Belarus, where a very severe problem with MDR-TB was demonstrated in a recent drug resistant survey.
Aksana Zalutskaya, Maria Wijkander, Pontus Jureen, Alena Skrahina, Sven Hoffner   +4 more
doaj   +1 more source

Reevaluation of the Critical Concentration for Drug Susceptibility Testing of Mycobacterium tuberculosis against Pyrazinamide Using Wild-Type MIC Distributions and pncA Gene Sequencing [PDF]

, 2011
Jim Werngren, Erik Sturegård, Pontus Juréen, Kristian Ängeby, Sven Hoffner, Thomas Schön   +5 more
openalex   +1 more source