Results 11 to 20 of about 2,353 (181)

Pyrazinamide resistance and pncA gene mutations in Mycobacterium tuberculosis. [PDF]

Antimicrob Agents Chemother, 2008
ABSTRACT Thirty-four pyrazinamide-resistant and 37 pyrazinamide-susceptible Mycobacterium tuberculosis complex strains were analyzed for pncA gene mutations. None of the sensitive strains had any mutations, apart from silent mutations, whereas all but one resistant strain showed
Juréen P, Werngren J, Toro JC, Hoffner S.   +3 more
europepmc   +5 more sources

Association between pncA gene mutations, pyrazinamidase activity, and pyrazinamide susceptibility testing in Mycobacterium tuberculosis. [PDF]

Antimicrob Agents Chemother, 2014
ABSTRACT We determined MICs for, confirmed the presence of pncA mutations in, and performed pyrazinamidase testing on colonies (subclones) obtained from seven isolates that exhibited differential pyrazinamide (PZA) susceptibility.
Aono A, Chikamatsu K, Yamada H, Kato T, Mitarai S.   +4 more
europepmc   +5 more sources

Non-pncA Gene-Mutated but Pyrazinamide-Resistant Mycobacterium tuberculosis: Why Is That? [PDF]

J Clin Microbiol, 2017
ABSTRACT Pyrazinamide (PZA) is a key component for the effective treatment of drug-susceptible and PZA-susceptible multidrug-resistant (MDR PZA-S ) tuberculosis (TB). pncA gene mutations are usually detected in a clear majority (>90%) of PZA-resistant strains but obviously not in
Werngren J, Alm E, Mansjö M.
europepmc   +6 more sources

Rapid sequencing of the Mycobacterium tuberculosis pncA gene for detection of pyrazinamide susceptibility. [PDF]

J Clin Microbiol, 2014
ABSTRACT We developed a pyrazinamidase gene DNA-sequencing method to rapidly identify pyrazinamide resistance-causing mutations in GenoLyse-treated, smear-positive sputum specimens. The sensitivity and specificity were 90.9 and 100%, respectively, compared to those of MGIT drug susceptibility testing, after the exclusion of synonymous ...
Streicher EM, Maharaj K, York T, Van Heerden C, Barnard M, Diacon A, Mendel CM, Bosman ME, Hepple JA, Pym AS, Warren RM, van Helden PD.   +11 more
europepmc   +6 more sources

Analysis of pncA gene expression in pyrazinamide-resistant and multidrug-resistant Mycobacterium tuberculosis [PDF]

Xin yixue, 2023
Objective To compare the expression levels of pncA gene in pyrazinamide （PZA）-resistant and multidrug-resistant Mycobacterium tuberculosis before and after PZA drug stimulation， and to explore the relationship between PZA-resistant Mycobacterium ...
Wang Nan，, Yang Yu, Deng Ling, Wu Hui, Liu Zhihui, Meng Fanrong
doaj   +2 more sources

The Riemerella anatipestifer AS87_01735 Gene Encodes Nicotinamidase PncA, an Important Virulence Factor [PDF]

Applied and Environmental Microbiology, 2016
ABSTRACT Riemerella anatipestifer is a major bacterial pathogen that causes septicemic and exudative diseases in domestic ducks. In our previous study, we found that deletion of the AS87_01735 gene significantly decreased the bacterial virulence of R.
Xiaolan Wang, Beibei Liu, Yafeng Dou, Hongjie Fan, Shaohui Wang, Tao Li, Chan Ding, Shengqing Yu   +7 more
openalex   +3 more sources

Phenotypic and genotypic characterization of Mycobacterium tuberculosis pyrazinamide resistance—India, 2018–2020 [PDF]

Frontiers in Microbiology
Pyrazinamide (PZA) is a key first-line antituberculosis drug that plays an important role in eradicating persister Mycobacterium tuberculosis (TB) bacilli and shortening the duration of tuberculosis treatment.
Sembulingam Tamilzhalagan, Evanslin Santus Justin, Ashok Selvaraj, Karthick Venkateswaran, Arun Kumar Sivakumar, Suganthi Chittibabu, Heather P. McLaughlin, Patrick K. Moonan, Jonathan P. Smith, Sakthi Suba, Mukesh Kumar Sathya Narayanan, Christine S. Ho, Nishant Kumar, Srikanth P. Tripathy, Srikanth P. Tripathy, Siva K. Shanmugam, Patricia J. Hall-Eidson, Uma Devi Ranganathan   +17 more
doaj   +2 more sources

Detection of Pyrazinamide-Resistant Mycobacteriumtuberculosis by PCR-SSCP of pncA Gene [PDF]

Tuberculosis and Respiratory Diseases, 1998
Background : Recently the incidence of tuberculosis is increasing in many countries and control of the disease is further threatened by the emergence of multi-drug resistant tuberculosis. So rapid detection of drug resistance is very important. Pyrazinamide (PZA) is a first-line chemotherapeutic agent for tuberculosis.
Tae Sun Shim, Young Whan Kim, Jae Yong Chin, Chae Man Lim, Sang‐Do Lee, Younsuck Koh, Woo Sung Kim, Dong Soon Kim, Won‐Young Kim   +8 more
openalex   +2 more sources

Pyrazinamide resistance associated with pncA gene mutation in Mycobacterium tuberculosis in Japan [PDF]

Epidemiology and Infection, 2002
Thirty Japanese clinical isolates of Mycobacterium tuberculosis were analysed by pyrazinamide susceptibility testing and pyrazinamidase assay, as well as polymerase chain reaction for single-strand conformational polymorphism and direct sequencing of the gene encoding pyrazinamidase (pncA).
Toshiaki Endoh, A Yagihashi, Nobuyuki Uehara, Daisuke Kobayashi, Naoki Tsuji, Michiko Nakamura, S Hayashi, Nobuhiro Fujii, Naoki Watanabe   +8 more
openalex   +4 more sources

Identification of mutations in rpoB, pncA, embB, and ubiA genes among drug-resistant Mycobacterium tuberculosis clinical isolates from Iran [PDF]

Acta Microbiologica et Immunologica Hungarica, 2022
Abstract Mycobacterium tuberculosis resistant to effective first-line drugs (FLDs) has challenged national and global tuberculosis control programs. This study aimed to identify mutations in 4 genes related to rifampin, pyrazinamide, and ethambutol resistance among clinical isolates of M. tuberculosis from southwestern Iran.
Pejman Bakhtiyariniya, Azar Dokht Khosravi, Mohammad Hashemzadeh, Mohammad Savari   +3 more
openalex   +4 more sources