Results 21 to 30 of about 2,353 (181)

Gene Sequencing for Routine Verification of Pyrazinamide Resistance in Mycobacterium tuberculosis: a Role for pncA but Not rpsA [PDF]

Journal of Clinical Microbiology, 2012
ABSTRACT Pyrazinamide (PZA) is an important component of first-line therapy for the treatment of tuberculosis. Here, we evaluate targeted gene sequencing as a supplement to phenotypic PZA susceptibility testing of Mycobacterium tuberculosis . Routine sequencing of pncA , but not
David C. Alexander, H. Jennifer, Jennifer L. Guthrie, Joanne Blair, Pam Chedore, Frances Jamieson   +5 more
openalex   +3 more sources

pncA gene expression and prediction factors on pyrazinamide resistance in Mycobacterium tuberculosis. [PDF]

Tuberculosis (Edinb), 2013
Mutations in the pyrazinamidase (PZAse) coding gene, pncA, have been considered as the main cause of pyrazinamide (PZA) resistance in Mycobacterium tuberculosis. However, recent studies suggest there is no single mechanism of resistance to PZA. The pyrazinoic acid (POA) efflux rate is the basis of the PZA susceptibility Wayne test, and its quantitative
Sheen P, Lozano K, Gilman RH, Valencia HJ, Loli S, Fuentes P, Grandjean L, Zimic M.   +7 more
europepmc   +5 more sources

Molecular characterization of pncA gene mutations in Mycobacterium tuberculosis clinical isolates from China [PDF]

Epidemiology and Infection, 2000
A sample of 35 pyrazinamide (PZA)-resistant and 30 PZA-susceptible clinical isolates recovered from Beijing and Taiyuan City, China were characterized by SSCP and sequence analysis for mutations in the pncA gene that encodes the Mycobacterium tuberculosis PZase.
Lifang Hou, Douglas Osei‐Hyiaman, Z. Zhang, B. Wang, Adeline Yang, Keiko Kano   +5 more
openalex   +4 more sources

Intestinal Fut2 deficiency exacerbated alcohol-related liver disease by disrupting nicotinamide metabolism. [PDF]

Clin Transl Med
Loss of intestinal epithelial fucosylation exacerbates alcohol‐related liver injuries. Intestinal epithelial deletion of Fut2 disrupts nicotinamide (NAM) metabolism due to the decline in bacterial nicotinamidase (PncA). Supplemented with pncA overexpressed E.coli to restore gut PncA levels can improve liver damage in alcohol‐induced intestinal ...
Chen L, Yin Z, Shang L, Yuan H, Gao W, Wang S, Wang S, Hou X, Yang S, Chu H.   +9 more
europepmc   +2 more sources

Detection of Pyrazinamide Resisitance in Mycobacterium Tuberculosis by Sequencing of pncA Gene [PDF]

Tuberculosis and Respiratory Diseases, 2001
Background : Examining the biological susceptibility of Mycobacterium tuberculosis to pyrazinamide (PZA) in vitro is very difficult as PZA is inactive under normal culture conditions. The biological susceptibility test, an enzyme assay for Pzase activity, and a genetic test for pncA gene mutations, were performed in order to predict PZA resistance ...
Jee Yoon Hwang, Kyung Rok Kwak, Hye‐Kyung Park, Jiseok Lee, Sam Seok Park, Yun Seong Kim, Jung Yoo Lee, Chul Hun Chang, Min Ki Lee, Soon Kew Park   +9 more
openalex   +2 more sources

Identification, cloning, and expression of the Escherichia coli pyrazinamidase and nicotinamidase gene, pncA [PDF]

Antimicrobial Agents and Chemotherapy, 1996
Pyrazinamide (PZA) is one of the three most important drugs for treatment of Mycobacterium tuberculosis infections. The antibacterial activity of PZA requires a bacterial enzyme, pyrazinamidase (PZAase), which hydrolyzes PZA to form pyrazinoic acid and ammonia. Most PZA-resistant clinical M. tuberculosis isolates lack PZAase activity.
Richard Frothingham, Winifred A. Meeker-O’Connell, Elizabeth A. Talbot, John W. George, Kenneth N. Kreuzer   +4 more
openalex   +3 more sources

Molecular investigation of mutation in PncA gene for pyrazinamide resistance in Mycobacterium tuberculosis: an in silico approach

Первое Российско-Китайское рабочее совещание по интегративной биоинформатике и компьютерной системной биологии, 2018
D. Ruban Durairaj, Shanmughavel Piramanayagam, M Whitfield, H Soeters, R Warren, T York, S Sampson, E Streicher, P Van Helden, A Van Rie, P Juren, J Werngren, J.-C Toro, S Hoffner   +13 more
openalex   +2 more sources

Mechanisms of Antibiotic Resistance and Novel Therapeutic Approaches for <i>Mycobacterium tuberculosis</i>: A Narrative Review With a Focus on Tuberculosis Mutations in Iran. [PDF]

Health Sci Rep
ABSTRACT Background and Aims Tuberculosis, caused by Mycobacterium tuberculosis (M. tuberculosis), is a highly contagious disease and one of the leading causes of mortality globally. The emergence and dissemination of multidrug‐resistant (MDR) strains of M. tuberculosis have become a major concern, especially in developing countries.
Bodaghi AB, Shariati A, Shamseddin J, Bodaghi AB, Rezaei M, Farahani A.   +5 more
europepmc   +2 more sources

In silico Analysis of Novel Mutation ala102pro Targeting pncA Gene of M. Tuberculosis [PDF]

Journal of Computer Science & Systems Biology, 2013
This study reports on the structural and functional basis of pyrazinamide (PZA) resistance conferred by a novel mutation Ala102Pro in pncA gene as sequenced from a PZA resistant Mycobacterium tuberculosis strain. Molecular modeling studies of Wild Type (WT) and Mutant Type (MT) of Pyrazinamidase (PZase) showed the mutation at Ala102Pro does not impact ...
Dhanurekha Lakshmipathy
openalex   +2 more sources

1474Genotypic Characterization of pncA Gene in Patients with Multidrug-resistant Tuberculosis from South Africa

Open Forum Infectious Diseases, 2014
Rationale: Pyrazinamide (PZA) is an essential component of empiric firstand second-line tuberculosis (TB) treatment regimens. However, PZA drug susceptibility testing (DST) is difficult to perform and not routinely available. Genetic mutations in the pncA gene of Mycobacterium tuberculosis (Mtb) commonly confer PZA resistance, but limited data on the ...
Salim Allana, Barun Mathema, James C.M. Brust, Thuli Mthiyane, Koleka Mlisana, Pravi Moodley, Sarita Shah, Neel R. Gandhi   +7 more
openalex   +2 more sources