Results 1 to 10 of about 6,527,208 (145)

Pigs with an INS point mutation derived from zygotes electroporated with CRISPR/Cas9 and ssODN [PDF]

Frontiers in Cell and Developmental Biology, 2023
Just one amino acid at the carboxy-terminus of the B chain distinguishes human insulin from porcine insulin. By introducing a precise point mutation into the porcine insulin (INS) gene, we were able to generate genetically modified pigs that secreted ...
Fuminori Tanihara, Maki Hirata, Maki Hirata, Zhao Namula, Zhao Namula, Lanh Thi Kim Do, Lanh Thi Kim Do, Naoaki Yoshimura, Naoaki Yoshimura, Qingyi Lin, Qingyi Lin, Koki Takebayashi, Koki Takebayashi, Tetsushi Sakuma, Takashi Yamamoto, Takeshige Otoi, Takeshige Otoi   +16 more
doaj   +2 more sources

A case of adrenomyeloneuropathy caused by a novel point mutation in the ABCD1 gene and functional verification [PDF]

Frontiers in Genetics
Adrenoleukodystrophy is a rare neurogenetic disease, and adrenomyeloneuropathy is the most common phenotype in adults. The clinical data of a patient with adrenoleukodystrophy and spinal-peripheral neuropathy caused by a novel point mutation in exon 4 of
Xiaoxue Shi, Xiaoxue Shi, Xiaoxue Shi, Xuelin Qi, Xuelin Qi, Jinhua Zheng, Jinhua Zheng, Jinhua Zheng, Jianjun Ma, Jianjun Ma, Jianjun Ma, Dongsheng Li, Dongsheng Li, Dongsheng Li   +13 more
doaj   +2 more sources

Salmonella enteritidis acquires phage resistance through a point mutation in rfbD but loses some of its environmental adaptability [PDF]

Veterinary Research
Phage therapy holds promise as an alternative to antibiotics for combating multidrug-resistant bacteria. However, host bacteria can quickly produce progeny that are resistant to phage infection.
Yukun Zeng, Ping Li, Shenglong Liu, Mangmang Shen, Yuqing Liu, Xin Zhou   +5 more
doaj   +2 more sources

Biosensors for Point Mutation Detection [PDF]

Frontiers in Bioengineering and Biotechnology, 2021
Hanlin Jiang, Hui Xi, Mario Juhas, Yang Zhang   +3 more
doaj   +2 more sources

Base Editing in Peanut Using CRISPR/nCas9

Frontiers in Genome Editing, 2022
Peanut (Arachis hypogaea L.), an allotetraploid legume of the Fabaceae family, is able to thrive in tropical and subtropical regions and is considered as a promising oil seed crop worldwide.
Anjanasree K. Neelakandan, Binita Subedi, Sy M. Traore, Papias Binagwa, David A. Wright, Guohao He   +5 more
doaj   +1 more source

Variability of Iron Load in Patients of Sickle Cell Anaemia (HbSS): A study from Eastern India [PDF]

Journal of Clinical and Diagnostic Research, 2017
Introduction: Sickle Cell Anaemia (SCA) is one of the commonest haemoglobinopathies due to a point mutation (A→T) of the b-globin gene. Out of five haplotypes, the Arab-Indian haplotype present in India is one of the least severe phenotype and least ...
Pranati Mohanty, Rabindra Kumar Jena, Sudha Sethy   +2 more
doaj   +1 more source

Tryptophan residue of plasmid-encoded Pgp3 is important for Chlamydia muridarum to induce hydrosalpinx in mice

Frontiers in Microbiology, 2023
The crucial role of plasmid-encoded protein Pgp3 in Chlamydia pathogenesis has been demonstrated in various animal models. Previous studies have revealed that the Pgp3-deficient C.
Yumeng Huang, Haoqing Wu, Yina Sun, Yuanjun Liu   +3 more
doaj   +1 more source

Mutation-driven parallel evolution in emergence of ACE2-utilizing sarbecoviruses

Frontiers in Microbiology, 2023
Mutation and recombination are two major genetic mechanisms that drive the evolution of viruses. They both exert an interplay during virus evolution, in which mutations provide a first ancestral source of genetic diversity for subsequent recombination ...
Bin Gao, Shunyi Zhu
doaj   +1 more source

Surface charge changes in spike RBD mutations of SARS-CoV-2 and its variant strains alter the virus evasiveness via HSPGs: A review and mechanistic hypothesis

Frontiers in Public Health, 2022
With the COVID-19 pandemic continuing, more contagious SARS-CoV-2 variants, including Omicron, have been emerging. The mutations, especially those that occurred on the spike (S) protein receptor-binding domain (RBD), are of significant concern due to ...
Zhongyun Zhang, Zhongyun Zhang, Juan Zhang, Juan Zhang, Jiqiu Wang, Jiqiu Wang   +5 more
doaj   +1 more source

Specific correction of pyruvate kinase deficiency-causing point mutations by CRISPR/Cas9 and single-stranded oligodeoxynucleotides

Frontiers in Genome Editing, 2023
Pyruvate kinase deficiency (PKD) is an autosomal recessive disorder caused by mutations in the PKLR gene. PKD-erythroid cells suffer from an energy imbalance caused by a reduction of erythroid pyruvate kinase (RPK) enzyme activity. PKD is associated with
Sara Fañanas-Baquero, Sara Fañanas-Baquero, Matías Morín, Sergio Fernández, Isabel Ojeda-Perez, Isabel Ojeda-Perez, Mercedes Dessy-Rodriguez, Mercedes Dessy-Rodriguez, Miruna Giurgiu, Miruna Giurgiu, Juan A. Bueren, Juan A. Bueren, Miguel Angel Moreno-Pelayo, Jose Carlos Segovia, Jose Carlos Segovia, Oscar Quintana-Bustamante, Oscar Quintana-Bustamante   +16 more
doaj   +1 more source