Results 11 to 20 of about 6,585,062 (339)
Point Mutation of Hoxd12 in Mice [PDF]
Yonsei Medical Journal, 2008 Genes of the HoxD cluster play a major role in vertebrate limb development, and changes that modify the Hoxd12 locus affect other genes also, suggesting that HoxD function is coordinated by a control mechanism involving multiple genes during limb morphogenesis. In this study, mutant phenotypes were produced by treatment of mice with a chemical mutagen,
Cho, JW +5 more
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Base Editing in Peanut Using CRISPR/nCas9
Frontiers in Genome Editing, 2022 Peanut (Arachis hypogaea L.), an allotetraploid legume of the Fabaceae family, is able to thrive in tropical and subtropical regions and is considered as a promising oil seed crop worldwide.
Anjanasree K. Neelakandan +5 more
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Pathogens, 2023 The CS21 pilus produced by enterotoxigenic Escherichia coli (ETEC) is involved in adherence to HT-29 intestinal cells. The CS21 pilus assembles proteins encoded by 14 genes clustered into the lng operon. Aim. This study aimed to determine whether E. coli
Karina Espinosa-Mazariego +9 more
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Frontiers in Microbiology, 2023 The crucial role of plasmid-encoded protein Pgp3 in Chlamydia pathogenesis has been demonstrated in various animal models. Previous studies have revealed that the Pgp3-deficient C.
Yumeng Huang +3 more
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Pyruvic Acid Production from Sucrose by Escherichia coli Pyruvate Dehydrogenase Variants
Fermentation, 2023 Sucrose is an abundant, cheap, and renewable carbohydrate which makes it an attractive feedstock for the biotechnological production of chemicals. Escherichia coli W, one of the few safe E.
W. Chris Moxley, Mark A. Eiteman
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Frontiers in Medicine, 2023 BackgroundTailored therapy has been widely used for patients with Helicobacter pylori (H. pylori) infection in South Korea. Herein, we evaluated the treatment outcomes of tailored clarithromycin-based triple therapy (TT) in patients infected with H ...
Seong Hyun Cho +5 more
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Mutation-driven parallel evolution in emergence of ACE2-utilizing sarbecoviruses
Frontiers in Microbiology, 2023 Mutation and recombination are two major genetic mechanisms that drive the evolution of viruses. They both exert an interplay during virus evolution, in which mutations provide a first ancestral source of genetic diversity for subsequent recombination ...
Bin Gao, Shunyi Zhu
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Pigs with an INS point mutation derived from zygotes electroporated with CRISPR/Cas9 and ssODN
Frontiers in Cell and Developmental Biology, 2023 Just one amino acid at the carboxy-terminus of the B chain distinguishes human insulin from porcine insulin. By introducing a precise point mutation into the porcine insulin (INS) gene, we were able to generate genetically modified pigs that secreted ...
Fuminori Tanihara +16 more
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Detection of point mutations in T lymphocytes [PDF]
Clinical Chemistry, 1995 Abstract We exposed experimental animals to a series of alkylating agents that induced mutations at the X-linked hprt gene of T lymphocytes. We then isolated the mutant cells and analyzed the molecular nature of the mutations by amplification of hprt cDNA sequences with the use of reverse transcriptase PCR followed by DNA sequence ...
Paul H.M. Lohman +5 more
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Frontiers in Genome Editing, 2023 Pyruvate kinase deficiency (PKD) is an autosomal recessive disorder caused by mutations in the PKLR gene. PKD-erythroid cells suffer from an energy imbalance caused by a reduction of erythroid pyruvate kinase (RPK) enzyme activity. PKD is associated with
Sara Fañanas-Baquero +16 more
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