Results 151 to 160 of about 283,934 (290)

Nuclear RNA Surveillance in \u3cem\u3eSaccharomyces cerevisiae\u3c/em\u3e: Trf4p-dependent Polyadenylation of Nascent Hypomethylated tRNA and an Aberrant Form of 5S rRNA [PDF]

, 2006
1-Methyladenosine modification at position 58 of tRNA is catalyzed by a two-subunit methyltransferase composed of Trm6p and Trm61p in Saccharomyces cerevisiae.
Anderson, James T., Kadaba, Sujatha A., Wang, Xuying   +2 more
core   +1 more source

Ascorbic Acid Modulates Collagen Properties in Glucocorticoid‐Induced Osteoporotic Bone: Insights into Chemical, Mechanical, and Biological Regulation

Advanced Healthcare Materials, EarlyView.
Osteoporosis from long‐term glucocorticoid (GIOP) use elevates susceptibility to fracture. This study shows GCs impair ascorbic acid (AA) metabolism in osteoblasts, collagen synthesis and extracellular matrix integrity. AA enhanced collagen biochemical and mechanical properties and restored osteoblast and endothelial function. These findings underscore
Micaila DE Curtis, Yoontae Kim, Rahul Patil, Xinran Zhang, Meth Jayatilake, Orelle Bulgin, Peter Lialios, Sana Surrency, Soraya Tarrah, Argyris K. Lazaris, Alison Grafton, James Williams IV, Seyedeh Boshra Nouraie, Maria Bizaki, Penelope Xeron, Edward Van Keuren, Jonathan E. Seppala, Evagelia C. Laiakis, Joanna B. Kitlinska, Stella Alimperti   +19 more
wiley   +1 more source

RNA degradation triggered by decapping is largely independent of initial deadenylation

The EMBO Journal
RNA stability, important for eukaryotic gene expression, is thought to depend on deadenylation rates, with shortened poly(A) tails triggering decapping and 5′ to 3′ degradation.
Léna Audebert, Frank Feuerbach, Mostafa Zedan, Alexandra P Schürch, Laurence Decourty, Abdelkader Namane, Emmanuelle Permal, Karsten Weis, Gwenaël Badis, Cosmin Saveanu   +9 more
doaj   +1 more source

Prognostic insights, immune infiltration, and therapeutic response: Cytoplasmic poly(A) tail regulators in hepatocellular carcinoma

Molecular Therapy: Oncology
The presence of a poly(A) tail is indispensable for the post-transcriptional regulation of gene expression in cancer. This dynamic and modifiable feature of transcripts is under the control of various nuclear and cytoplasmic proteins. This study aimed to
Yi Liu, Yan Huang, Yunting Le, Yating Gao, Hui Wang, Jing Yang, Jialin Wang, Chaoxia Zou, Qiang Li   +8 more
doaj   +1 more source

Liquid Chromatography Methods for Analysis of mRNA Poly(A) Tail Length and Heterogeneity. [PDF]

Anal Chem, 2023
Gilar M, Doneanu C, Gaye MM.
europepmc   +1 more source

Spherical Skin Model: Stratified Co‐Culture of Fibroblasts and Keratinocytes on Spherical Beads Toward Compound Screening

Advanced Healthcare Materials, EarlyView.
Models of the human skin must combine the relevant biological contents and suitable biomaterials with the correct spatial organization. Performing compound screening on such in vitro models also requires fast and reproducible production methods of the models.
Elisa Lenzi, Nadia Vertti‐Quintero, Julien Husson, Anne‐Laure Bulteau, Carine Nizard, Karl Pays, Sébastien Sart, Charles N. Baroud   +7 more
wiley   +1 more source

CCR4-NOT differentially controls host versus virus poly(a)-tail length and regulates HCMV infection. [PDF]

EMBO Rep, 2023
Burgess HM, Grande R, Riccio S, Dinesh I, Winkler GS, Depledge DP, Mohr I.   +6 more
europepmc   +1 more source

Bioengineered Lymphatic Vessels in Synthetic Matrices to Study Breast Cancer Cell Functions

Advanced Healthcare Materials, EarlyView.
Lymphatic vessels are involved in cancer metastasis. To study the interplay between metastasizing cancer cells and lymphatic vessels under highly reproducible conditions, advanced in vitro models are required. In this work, 3D lymphatic networks are formed in biomimetic hydrogels and their interactions with invasive and non‐invasive cancer cell‐lines ...
Rodi Odabasi, Lisa A. Krattiger, Magdalini Kanari, Mira A. Jacobs, Lukas O. Moser, Mark W. Tibbitt, Cornelia Halin, Martin Ehrbar   +7 more
wiley   +1 more source

The Floor‐Ceiling‐Chip, or 2 × 2D = Pseudo‐3D—Approaching 3D Cell Morphology and Organization between Two Opposing 2D Substrates with Cell‐Adhesive Protein Micropatterns

Advanced Healthcare Materials, EarlyView.
Here, we present a novel 3D cell patterning and culture platform. The “Floor‐Ceiling‐Chip” (FC‐Chip) consists of two opposing track‐etched membranes, creating a pseudo‐3D microenvironment for the cells in between. By providing the membranes with micropatterned cell‐adhesive islands of varying geometries and sizes, the FC‐Chip enables control over cell ...
Urandelger Tuvshindorj, Francesca Giacomini, Esra Güben Kaçmaz, Tim ten Brink, Lorenzo Moroni, Zeinab Tahmasebi Birgani, Clemens van Blitterswijk, Pamela Habibović, Stefan Giselbrecht, Jan de Boer, Roman Truckenmüller   +10 more
wiley   +1 more source

Rational design of an artificial tethered enzyme for non-templated post-transcriptional mRNA polyadenylation by the second generation of the C3P3 system

Scientific Reports
We have previously introduced the first generation of C3P3, an artificial system that allows the autonomous in-vivo production of mRNA with m7GpppN-cap. While C3P3-G1 synthesized much larger amounts of capped mRNA in human cells than conventional nuclear
Marine Le Boulch, Eric Jacquet, Naïma Nhiri, Maya Shmulevitz, Philippe H. Jaïs   +4 more
doaj   +1 more source