Results 181 to 190 of about 13,318 (225)

Apoptosis in the Absence of Poly- (ADP-ribose) Polymerase

Biochemical and Biophysical Research Communications, 1997
Cleavage of poly-(ADP-ribose) polymerase is a process occurring early during the execution phase of apoptosis. Although in many experimental systems PARP cleavage indicates a point of no return, the significance of this proteolytic step for apoptosis remains unclear.
M, Leist, B, Single, G, Künstle, C, Volbracht, H, Hentze, P, Nicotera   +5 more
openaire   +2 more sources

Structure and function of poly(ADP-ribose) polymerase

Molecular and Cellular Biochemistry, 1994
Poly(ADP-ribose) polymerase (PARP) participates in the intricate network of systems developed by the eukaryotic cell to cope with the numerous environmental and endogenous genetoxic agents. Cloning of the PARP gene has allowed the development of genetic and molecular approaches to elucidate the structure and the function of this abundant and highly ...
Murcia, G, Schreiber, V, Molinete, M, Saulier, B, Poch, O, Masson, M, Niedergang, C, Murcia, J   +7 more
openaire   +3 more sources

Inhibition of poly(ADP-ribose) polymerase by arsenite

Mutation Research/Reviews in Mutation Research, 1997
Inorganic arsenic is considered a human carcinogen based principally on epidemiological evidence. Unlike most initiating chemicals, arsenic is inactive or extremely weak in its ability to directly induce gene mutations. Arsenite has been shown, however, to enhance mutagenicity when present with other agents such as UV radiation.
J W, Yager, J K, Wiencke
openaire   +2 more sources

Cytological detection of poly (ADP-ribose) polymerase

Experimental Cell Research, 1976
Abstract An attempt was made to demonstrate poly (ADP-ribose) polymerase cytologically. In vitro incorporation from the nucleotide, [ 3 H]NAD was detected in frozen sections of onion embryo and meristematic tissue by autoradiography. In meristematic tissue, there was a correlation between the number of cells displaying intensein vitro incorporation ...
J F, Payne, A K, Bal
openaire   +2 more sources

Activity Assays for Poly-ADP Ribose Polymerase

2004
Poly(ADP-ribose) polymerase (PARP-1) is a nuclear enzyme that has traditionally been thought to require discontinuous or "damaged" DNA (dcDNA) as a coenzyme, a preconception that has limited research mainly to its role in cell pathology, i.e., DNA repair and apoptosis.
Eva, Kirsten, Ernest, Kun, Jerome, Mendeleyev, Charles P, Ordahl   +3 more
openaire   +2 more sources

Regulatory mechanisms of poly(ADP-ribose) polymerase

Molecular and Cellular Biochemistry, 1999
Here, we describe the latest developments on the mechanistic characterization of poly(ADP-ribose) polymerase (PARP) [EC 2.4.2.30], a DNA-dependent enzyme that catalyzes the synthesis of protein-bound ADP-ribose polymers in eucaryotic chromatin. A detailed kinetic analysis of the automodification reaction of PARP in the presence of nicked dsDNA ...
R, Alvarez-Gonzalez, T A, Watkins, P K, Gill, J L, Reed, H, Mendoza-Alvarez   +4 more
openaire   +2 more sources

Interaction of poly(ADP-ribose)polymerase with DNA polymerase ?

Molecular and Cellular Biochemistry, 1994
Homogeneously purified poly(ADP-ribose) polymerase (PARP) specifically stimulated the activity of immunoaffinity-purified calf or human DNA polymerase alpha by about 6 to 60-fold. Apparently, poly(ADP-ribosyl)ation of DNA polymerase alpha was not necessary for the stimulation.
S, Yoshida, C M, Simbulan
openaire   +2 more sources

Inhibition of poly(ADP-ribose) polymerase in cancer

Current Opinion in Pharmacology, 2006
Inhibition of the DNA repair enzyme poly(ADP-ribose) polymerase-1 (PARP-1) has been extensively investigated in the pre-clinical setting as a strategy for chemo- or radio-potentiation. Recent evidence has suggested that PARP inhibitors might be active as single agents in certain rare inherited cancers that carry DNA repair defects.
openaire   +2 more sources

Detection of poly(ADP-ribose) polymerase and its reaction product poly(ADP-ribose) by immunocytochemistry

The Histochemical Journal, 1996
Poly(ADP-ribose) polymerase catalyses the formation of ADP-ribose polymers covalently attached to various nuclear proteins, using NAD+ as substrate. The activity of this enzyme is strongly stimulated upon binding to DNA single or double strand breaks. Poly(ADP-ribosyl)ation is an immediate cellular response to DNA damage and is thought to be involved ...
J H, Küpper, L, van Gool, M, Müller, A, Bürkle   +3 more
openaire   +2 more sources

Poly (ADP-ribose) polymerase 1

2022
Poly (ADP-ribose) polymerase 1 is a key player in DNA repair and transcription regulation. The role of PARP1 in DNA damage recognition and protein signaling in response make it integral to genome stability. Beyond this, it regulates gene expression through chromatin remodeling and transcription factor binding.
Fletcher E Bain, Maria Spies, Marc Wold, Adrian Elcock, Anna Malkova, Miles Pufall, Lori Wallrath   +6 more
openaire   +1 more source