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Structure and function of poly(ADP-ribose) polymerase
Molecular and Cellular Biochemistry, 1994Poly(ADP-ribose) polymerase (PARP) participates in the intricate network of systems developed by the eukaryotic cell to cope with the numerous environmental and endogenous genetoxic agents. Cloning of the PARP gene has allowed the development of genetic and molecular approaches to elucidate the structure and the function of this abundant and highly ...
Murcia, G +7 more
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Purification of Recombinant Poly(ADP-Ribose) Polymerases
2011The purification of Poly(ADP-ribose) polymerases from overexpressing cells (Sf9 insect cells, Escherichia coli) has been updated to a fast and reproducible three chromatographic steps protocol. After cell lysis, proteins from the crude extract are separated on a Heparine Sepharose™ column. The PARP-containing fractions are then affinity purified on a 3-
Amé, Jean-Christophe +3 more
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Inhibition of poly(ADP-ribose) polymerase by arsenite
Mutation Research/Reviews in Mutation Research, 1997Inorganic arsenic is considered a human carcinogen based principally on epidemiological evidence. Unlike most initiating chemicals, arsenic is inactive or extremely weak in its ability to directly induce gene mutations. Arsenite has been shown, however, to enhance mutagenicity when present with other agents such as UV radiation.
J W, Yager, J K, Wiencke
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Cytological detection of poly (ADP-ribose) polymerase
Experimental Cell Research, 1976Abstract An attempt was made to demonstrate poly (ADP-ribose) polymerase cytologically. In vitro incorporation from the nucleotide, [ 3 H]NAD was detected in frozen sections of onion embryo and meristematic tissue by autoradiography. In meristematic tissue, there was a correlation between the number of cells displaying intensein vitro incorporation ...
J F, Payne, A K, Bal
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Activity Assays for Poly-ADP Ribose Polymerase
2004Poly(ADP-ribose) polymerase (PARP-1) is a nuclear enzyme that has traditionally been thought to require discontinuous or "damaged" DNA (dcDNA) as a coenzyme, a preconception that has limited research mainly to its role in cell pathology, i.e., DNA repair and apoptosis.
Eva, Kirsten +3 more
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Regulatory mechanisms of poly(ADP-ribose) polymerase
Molecular and Cellular Biochemistry, 1999Here, we describe the latest developments on the mechanistic characterization of poly(ADP-ribose) polymerase (PARP) [EC 2.4.2.30], a DNA-dependent enzyme that catalyzes the synthesis of protein-bound ADP-ribose polymers in eucaryotic chromatin. A detailed kinetic analysis of the automodification reaction of PARP in the presence of nicked dsDNA ...
R, Alvarez-Gonzalez +4 more
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Interaction of poly(ADP-ribose)polymerase with DNA polymerase ?
Molecular and Cellular Biochemistry, 1994Homogeneously purified poly(ADP-ribose) polymerase (PARP) specifically stimulated the activity of immunoaffinity-purified calf or human DNA polymerase alpha by about 6 to 60-fold. Apparently, poly(ADP-ribosyl)ation of DNA polymerase alpha was not necessary for the stimulation.
S, Yoshida, C M, Simbulan
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Inhibition of poly(ADP-ribose) polymerase in cancer
Current Opinion in Pharmacology, 2006Inhibition of the DNA repair enzyme poly(ADP-ribose) polymerase-1 (PARP-1) has been extensively investigated in the pre-clinical setting as a strategy for chemo- or radio-potentiation. Recent evidence has suggested that PARP inhibitors might be active as single agents in certain rare inherited cancers that carry DNA repair defects.
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The Histochemical Journal, 1996
Poly(ADP-ribose) polymerase catalyses the formation of ADP-ribose polymers covalently attached to various nuclear proteins, using NAD+ as substrate. The activity of this enzyme is strongly stimulated upon binding to DNA single or double strand breaks. Poly(ADP-ribosyl)ation is an immediate cellular response to DNA damage and is thought to be involved ...
J H, Küpper +3 more
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Poly(ADP-ribose) polymerase catalyses the formation of ADP-ribose polymers covalently attached to various nuclear proteins, using NAD+ as substrate. The activity of this enzyme is strongly stimulated upon binding to DNA single or double strand breaks. Poly(ADP-ribosyl)ation is an immediate cellular response to DNA damage and is thought to be involved ...
J H, Küpper +3 more
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Poly (ADP-ribose) polymerase 1
2022Poly (ADP-ribose) polymerase 1 is a key player in DNA repair and transcription regulation. The role of PARP1 in DNA damage recognition and protein signaling in response make it integral to genome stability. Beyond this, it regulates gene expression through chromatin remodeling and transcription factor binding.
Fletcher E Bain +6 more
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